LOCUS       sequence-247957-        8300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM 
            N-terminus, GSAx9 linker).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Lrig1 expression prospectively identifies stem cells in the 
            ventricular-subventricular zone that are neurogenic throughout adult
            life.
  JOURNAL   Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5.
  PUBMED    32183906
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neural 
            Dev."; date: "2020-03-17"; volume: "15(1):3. doi"; pages: " 
            10.1186/s13064-020-00139-5"
COMMENT     Sequence Label: pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
FEATURES             Location/Qualifiers
     source          1..8300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1734..2444
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             2466..2519
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2535..2537,2538..3248)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2535..3248
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2565..2593)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3188..3208
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4365..5300
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4413..5306
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(5318..5337)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5383..5438
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5437..5456)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5799..5815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5799..5815)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5812..5834)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5823..5839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5847..5853,5854..5871,5872..5877))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5892..5913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5972..6167
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6018..6153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6173..6307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6210..6229)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6264..6283
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6375..6392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6546..7134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6626..6645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7305..8096,8097..8165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7928..7947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8166..8270)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc ggggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gccatggtgt
     1741 ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     1801 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     1861 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     1921 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     1981 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     2041 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     2101 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     2161 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     2221 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata
     2281 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     2341 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     2401 ccagatactg cgacctccct agcaaactgg ggcacaagct taatggcggc tccggcggcc
     2461 gctccgaggg ccgcggcagc ctgctgacat gcggcgacgt ggaagagaac cctggccctg
     2521 ctcctggcag cacaatgagc aaaggagaag aacttttcac tggagttgtc ccaattcttg
     2581 ttgaattaga tggtgatgtt aatgggcaca aattttctgt ccgtggagag ggtgaaggtg
     2641 atgctacaaa cggaaaactc acccttaaat ttatttgcac tactggaaaa ctacctgttc
     2701 cgtggccaac acttgtcact actctgacct atggtgttca atgcttttcc cgttatccgg
     2761 atcacatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac
     2821 gcactatatc tttcaaagat gacgggacct acaagacgcg tgctgaagtc aagtttgaag
     2881 gtgataccct tgttaatcgt atcgagttaa agggtattga ttttaaagaa gatggaaaca
     2941 ttcttggaca caaactcgag tacaacttta actcacacaa tgtatacatc acggcagaca
     3001 aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacgttgaa gatggttccg
     3061 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     3121 cagacaacca ttacctgtcg acacaatctg tcctttcgaa agatcccaac gaaaagcgtg
     3181 accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc
     3241 tctacaaagg aagcgctggc tccgccgcag ggtctgggag tgccggctcc gctgcaggaa
     3301 gcggatctgc cggcagcgca gctgggtcca acctgctgac tgtgcaccaa aacctgcctg
     3361 ccctccctgt ggatgccacc tctgatgaag tcaggaagaa cctgatggac atgttcaggg
     3421 acaggcaggc cttctctgaa cacacctgga agatgctcct gtctgtgtgc agatcctggg
     3481 ctgcctggtg caagctgaac aacaggaaat ggttccctgc tgaacctgag gatgtgaggg
     3541 actacctcct gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa cagcacctgg
     3601 gccagctcaa catgctgcac aggagatctg gcctgcctcg cccttctgac tccaatgctg
     3661 tgtccctggt gatgaggaga atcagaaagg agaatgtgga tgctggggag agagccaagc
     3721 aggccctggc ctttgaacgc actgactttg accaagtcag atccctgatg gagaactctg
     3781 acagatgcca ggacatcagg aacctggcct tcctgggcat tgcctacaac accctgctgc
     3841 gcattgccga aattgccaga atcagagtga aggacatctc ccgcaccgat ggtgggagaa
     3901 tgctgatcca cattggcagg accaagaccc tggtgtccac agctggtgtg gagaaggccc
     3961 tgtccctggg ggttaccaag ctggtggaga gatggatctc tgtgtctggt gtggctgatg
     4021 accccaacaa ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc ccttctgcca
     4081 cctcccaact gtccacccgg gccctggaag ggatctttga ggccacccac cgcctgatct
     4141 atggtgccaa ggatgactct gggcagagat acctggcctg gtctggccac tctgccagag
     4201 tgggtgctgc cagggacatg gccagggctg gtgtgtccat ccctgaaatc atgcaggctg
     4261 gtggctggac caatgtgaac attgtgatga actacatcag aaacctggac tctgagactg
     4321 gggccatggt gaggctgcta gaggatgggg acctcgagcc atctgctgga gacatgagag
     4381 ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac agcctggcct
     4441 tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc cccatactct
     4501 attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc ttactgacca
     4561 acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg ccaggctttg
     4621 tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta gagatcctga
     4681 tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt gctcctaact
     4741 tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc
     4801 tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag tttgtgtgcc
     4861 tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc accctgaagt
     4921 ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac actttgatcc
     4981 acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg gcccagctcc
     5041 tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat ctgtacagca
     5101 tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg gacgcccacc
     5161 gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac caaagccact
     5221 tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc acgggggagg
     5281 cagagggttt ccctgccaca gcttgaggcg cgccggatct ttttccctct gccaaaaatt
     5341 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     5401 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     5461 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     5521 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     5581 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     5641 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     5701 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     5761 gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     5821 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5881 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5941 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc
     6001 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6061 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6121 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt
     6181 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6241 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6301 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6961 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7321 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7381 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7441 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7501 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7561 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7621 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7681 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7741 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7801 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7861 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7921 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7981 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8041 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8101 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8161 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8281 cgaaaagtgc cacctgggtc
//
