LOCUS       sequence-247977-        2421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-psbA 5'UTR
FEATURES             Location/Qualifiers
     source          1..2421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(627..644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(737..1325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(817..836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1499..2293)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2294..2398)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct catactaata aaaagccttc cattttctat
      421 tttgatttgt agaaaactag tgtgcttggg agtccctgat gattaaataa accaagattt
      481 taccaatgtg agaccggtac ctggagcaca agactggcct catgggcctt cctttcactg
      541 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aacatggtca tagctgtttc
      601 cttgcgtatt gggcgctctc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      661 gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1141 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1381 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1441 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttatt
     1501 agaaaaattc atccagcaga cgataaaacg caatacgctg gctatccggt gccgcaatgc
     1561 catacagcac cagaaaacga tccgcccatt cgccgcccag ttcttccgca atatcacggg
     1621 tggccagcgc aatatcctga taacgatccg ccacgcccag acggccgcaa tcaataaagc
     1681 cgctaaaacg gccattttcc accataatgt tcggcaggca cgcatcacca tgggtcacca
     1741 ccagatcttc gccatccggc atgctcgctt tcagacgcgc aaacagctct gccggtgcca
     1801 ggccctgatg ttcttcatcc agatcatcct gatccaccag gcccgcttcc atacgggtac
     1861 gcgcacgttc aatacgatgt ttcgcctgat gatcaaacgg acaggtcgcc gggtccaggg
     1921 tatgcagacg acgcatggca tccgccataa tgctcacttt ttctgccggc gccagatggc
     1981 tagacagcag atcctgaccc ggcacttcgc ccagcagcag ccaatcacgg cccgcttcgg
     2041 tcaccacatc cagcaccgcc gcacacggaa caccggtggt ggccagccag ctcagacgcg
     2101 ccgcttcatc ctgcagctcg ttcagcgcac cgctcagatc ggttttcaca aacagcaccg
     2161 gacgaccctg cgcgctcaga cgaaacaccg ccgcatcaga gcagccaatg gtctgctgcg
     2221 cccaatcata gccaaacaga cgttccaccc acgctgccgg gctacccgca tgcaggccat
     2281 cctgttcaat catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2341 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2401 catttccccg aaaagtgcca c
//
