LOCUS       sequence-247979-        2382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-Synthetic RBS
FEATURES             Location/Qualifiers
     source          1..2382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(588..605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(698..1286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(778..797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1460..2254)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2255..2359)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatg aagggcgcgc caggtctcat actaaattcg atagagatga aattggagct
      421 ctagagaatt cagttgtagg gagggatcca atgtgagacc attaattaac aacctgggcc
      481 tcatgggcct tcctttcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      541 taacatggtc atagctgttt ccttgcgtat tgggcgctct ccgcttcctc gctcactgac
      601 tcgctgcgct cggtcgttcg ggtaaagcct ggggtgccta atgagcaaaa ggccagcaaa
      661 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      721 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      781 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      841 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      901 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      961 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1021 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1081 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1141 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1201 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1261 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1321 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1381 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1441 aaacttggtc tgacagttat tagaaaaatt catccagcag acgataaaac gcaatacgct
     1501 ggctatccgg tgccgcaatg ccatacagca ccagaaaacg atccgcccat tcgccgccca
     1561 gttcttccgc aatatcacgg gtggccagcg caatatcctg ataacgatcc gccacgccca
     1621 gacggccgca atcaataaag ccgctaaaac ggccattttc caccataatg ttcggcaggc
     1681 acgcatcacc atgggtcacc accagatctt cgccatccgg catgctcgct ttcagacgcg
     1741 caaacagctc tgccggtgcc aggccctgat gttcttcatc cagatcatcc tgatccacca
     1801 ggcccgcttc catacgggta cgcgcacgtt caatacgatg tttcgcctga tgatcaaacg
     1861 gacaggtcgc cgggtccagg gtatgcagac gacgcatggc atccgccata atgctcactt
     1921 tttctgccgg cgccagatgg ctagacagca gatcctgacc cggcacttcg cccagcagca
     1981 gccaatcacg gcccgcttcg gtcaccacat ccagcaccgc cgcacacgga acaccggtgg
     2041 tggccagcca gctcagacgc gccgcttcat cctgcagctc gttcagcgca ccgctcagat
     2101 cggttttcac aaacagcacc ggacgaccct gcgcgctcag acgaaacacc gccgcatcag
     2161 agcagccaat ggtctgctgc gcccaatcat agccaaacag acgttccacc cacgctgccg
     2221 ggctacccgc atgcaggcca tcctgttcaa tcatactctt cctttttcaa tattattgaa
     2281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
