LOCUS       sequence-247981-        2509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-accD 5'UTR
FEATURES             Location/Qualifiers
     source          1..2509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(715..732)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(825..1413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(905..924)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1587..2381)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2382..2486)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggcctaggc gcgccatgag ctcggtctca tactaagtgt tcccccagat tcagaacttt
      421 ttttcaatac tcacaatcct tattagttaa taatcctagt gattggattt ctatgcttag
      481 tctgatagga aataagatat tcaaataaat aattttatag cgaatgacta ttcatctatt
      541 gtattttcat gcaaataggg ggcaagaaaa ctctaatgtg agaccggtac ctcttaatta
      601 actggcctca tgggccttcc tttcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      661 gctgcattaa catggtcata gctgtttcct tgcgtattgg gcgctctccg cttcctcgct
      721 cactgactcg ctgcgctcgg tcgttcgggt aaagcctggg gtgcctaatg agcaaaaggc
      781 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      841 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      901 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      961 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1021 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1081 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1141 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1201 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1261 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1321 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1381 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1441 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1501 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1561 tatgagtaaa cttggtctga cagttattag aaaaattcat ccagcagacg ataaaacgca
     1621 atacgctggc tatccggtgc cgcaatgcca tacagcacca gaaaacgatc cgcccattcg
     1681 ccgcccagtt cttccgcaat atcacgggtg gccagcgcaa tatcctgata acgatccgcc
     1741 acgcccagac ggccgcaatc aataaagccg ctaaaacggc cattttccac cataatgttc
     1801 ggcaggcacg catcaccatg ggtcaccacc agatcttcgc catccggcat gctcgctttc
     1861 agacgcgcaa acagctctgc cggtgccagg ccctgatgtt cttcatccag atcatcctga
     1921 tccaccaggc ccgcttccat acgggtacgc gcacgttcaa tacgatgttt cgcctgatga
     1981 tcaaacggac aggtcgccgg gtccagggta tgcagacgac gcatggcatc cgccataatg
     2041 ctcacttttt ctgccggcgc cagatggcta gacagcagat cctgacccgg cacttcgccc
     2101 agcagcagcc aatcacggcc cgcttcggtc accacatcca gcaccgccgc acacggaaca
     2161 ccggtggtgg ccagccagct cagacgcgcc gcttcatcct gcagctcgtt cagcgcaccg
     2221 ctcagatcgg ttttcacaaa cagcaccgga cgaccctgcg cgctcagacg aaacaccgcc
     2281 gcatcagagc agccaatggt ctgctgcgcc caatcatagc caaacagacg ttccacccac
     2341 gctgccgggc tacccgcatg caggccatcc tgttcaatca tactcttcct ttttcaatat
     2401 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2461 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
