LOCUS       sequence-247986-        3026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-pporRFP
FEATURES             Location/Qualifiers
     source          1..3026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1232..1249)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1342..1930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1422..1441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2104..2898)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2899..3003)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct caaatggctc tttcaaagca aagtggggtc
      421 aaagatgtaa tgaacaccga gcttcatatg gacgggatcg tcaatggaca cccctttgag
      481 ataaaaggaa aaggaaaggg aaacccgtac aagggtgtgc agaccatgaa gcttacagtc
      541 attaagggtg cgcctttgcc attttctatt gacattttgc tgcctcaaca catgtatgga
      601 agcaagccat ttattaagta tcctgagagt atcccagact acatcaagtt gtcatttccc
      661 gagggaatca catgggaaag gtccatgacc tttgaagatg gtgcagtgtg cactgtctct
      721 aacgactcca ggctcgatgg cgactctttc atctacgaag tcaggtttct tggcgtgaac
      781 tttccccgag atggacctgt tatgcagaag aaaacgcgag gctgggaccc gtccacagag
      841 agactgtatg agtgtggtgg gtggcagaga ggagatgtcc acatggcctt gaagttggag
      901 aacggtggcc attatacgtg cgacttcaaa actacttaca aatcaaagaa gggcttgaag
      961 gtgccaccgt atcacttcgt tgaccacaaa ctagatttac tgagccacaa caccgatggt
     1021 gctacctttg aagagtttga acaacgagaa attgcacatg cacatctttc taacttaccg
     1081 gtagcctaag ctttgagacc ggtacctgga gcacaagact ggcctcatgg gccttccttt
     1141 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaacat ggtcatagct
     1201 gtttccttgc gtattgggcg ctctccgctt cctcgctcac tgactcgctg cgctcggtcg
     1261 ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag caaaaggcca ggaaccgtaa
     1321 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1381 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1441 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1501 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1561 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1621 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1681 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1741 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1801 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1861 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1921 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1981 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2041 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2101 ttattagaaa aattcatcca gcagacgata aaacgcaata cgctggctat ccggtgccgc
     2161 aatgccatac agcaccagaa aacgatccgc ccattcgccg cccagttctt ccgcaatatc
     2221 acgggtggcc agcgcaatat cctgataacg atccgccacg cccagacggc cgcaatcaat
     2281 aaagccgcta aaacggccat tttccaccat aatgttcggc aggcacgcat caccatgggt
     2341 caccaccaga tcttcgccat ccggcatgct cgctttcaga cgcgcaaaca gctctgccgg
     2401 tgccaggccc tgatgttctt catccagatc atcctgatcc accaggcccg cttccatacg
     2461 ggtacgcgca cgttcaatac gatgtttcgc ctgatgatca aacggacagg tcgccgggtc
     2521 cagggtatgc agacgacgca tggcatccgc cataatgctc actttttctg ccggcgccag
     2581 atggctagac agcagatcct gacccggcac ttcgcccagc agcagccaat cacggcccgc
     2641 ttcggtcacc acatccagca ccgccgcaca cggaacaccg gtggtggcca gccagctcag
     2701 acgcgccgct tcatcctgca gctcgttcag cgcaccgctc agatcggttt tcacaaacag
     2761 caccggacga ccctgcgcgc tcagacgaaa caccgccgca tcagagcagc caatggtctg
     2821 ctgcgcccaa tcatagccaa acagacgttc cacccacgct gccgggctac ccgcatgcag
     2881 gccatcctgt tcaatcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     2941 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3001 gcgcacattt ccccgaaaag tgccac
//
