LOCUS       sequence-247989-        3077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-aphA6
FEATURES             Location/Qualifiers
     source          1..3077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1283..1300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1393..1981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1473..1492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2155..2949)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2950..3054)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatg gtctcaaatg gaattgccca atattattca acaatttatc ggaaacagcg
      421 ttttagagcc aaataaaatt ggtcagtcgc catcggatgt ttattctttt aatcgaaata
      481 atgaaacttt ttttcttaag cgatctagca ctttatatac agagacaaca tacagtgtct
      541 ctcgtgaagc gaaaatgttg agttggctct ctgagaaatt aaaggtgcct gaactcatca
      601 tgacttttca ggatgagcag tttgaattca tgatcactaa agcgatcaat gcaaaaccaa
      661 tttcagcgct ttttttaaca gaccaagaat tgcttgctat ctataaggag gcactcaatc
      721 tgttaaattc aattgctatt attgattgtc catttatttc aaacattgat catcggttaa
      781 aagagtcaaa attttttatt gataaccaac tccttgacga tatagatcaa gatgattttg
      841 acactgaatt atggggagat cataaaactt acctaagtct atggaatgag ttaaccgaga
      901 ctcgtgttga agaaagattg gttttttctc atggcgatat cacggatagt aatattttta
      961 tagataaatt caatgaaatt tattttttag atcttggtcg tgctgggtta gcagatgaat
     1021 ttgtagatat atcctttgtt gaacgttgcc taagagagga tgcatcggag gaaactgcga
     1081 aaatattttt aaagcattta aaaaatgata gacctgacaa aaggaattat tttttaaaac
     1141 ttgatgaatt gaattgagct ttgagaccct gggcctcatg ggccttcctt tcactgcccg
     1201 ctttccagtc gggaaacctg tcgtgccagc tgcattaaca tggtcatagc tgtttccttg
     1261 cgtattgggc gctctccgct tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa
     1321 agcctggggt gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1381 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1441 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1501 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1561 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1621 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1681 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1741 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1801 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1861 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1921 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1981 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2041 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2101 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttattagaa
     2161 aaattcatcc agcagacgat aaaacgcaat acgctggcta tccggtgccg caatgccata
     2221 cagcaccaga aaacgatccg cccattcgcc gcccagttct tccgcaatat cacgggtggc
     2281 cagcgcaata tcctgataac gatccgccac gcccagacgg ccgcaatcaa taaagccgct
     2341 aaaacggcca ttttccacca taatgttcgg caggcacgca tcaccatggg tcaccaccag
     2401 atcttcgcca tccggcatgc tcgctttcag acgcgcaaac agctctgccg gtgccaggcc
     2461 ctgatgttct tcatccagat catcctgatc caccaggccc gcttccatac gggtacgcgc
     2521 acgttcaata cgatgtttcg cctgatgatc aaacggacag gtcgccgggt ccagggtatg
     2581 cagacgacgc atggcatccg ccataatgct cactttttct gccggcgcca gatggctaga
     2641 cagcagatcc tgacccggca cttcgcccag cagcagccaa tcacggcccg cttcggtcac
     2701 cacatccagc accgccgcac acggaacacc ggtggtggcc agccagctca gacgcgccgc
     2761 ttcatcctgc agctcgttca gcgcaccgct cagatcggtt ttcacaaaca gcaccggacg
     2821 accctgcgcg ctcagacgaa acaccgccgc atcagagcag ccaatggtct gctgcgccca
     2881 atcatagcca aacagacgtt ccacccacgc tgccgggcta cccgcatgca ggccatcctg
     2941 ttcaatcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3001 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3061 tccccgaaaa gtgccac
//
