LOCUS       sequence-247991-        3125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-nptII
FEATURES             Location/Qualifiers
     source          1..3125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             394..1188
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(448..467)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(1331..1348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1441..2029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1521..1540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2203..2997)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2998..3102)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct caaatggttg aacaagatgg attgcacgca
      421 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
      481 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
      541 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
      601 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
      661 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
      721 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
      781 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
      841 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
      901 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gactcatggc
      961 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     1021 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     1081 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     1141 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc tttgagaccg
     1201 gtacctggag cacaagactg gcctcatggg ccttcctttc actgcccgct ttccagtcgg
     1261 gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc
     1321 tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc
     1381 ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1861 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2101 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2161 atcaatctaa agtatatatg agtaaacttg gtctgacagt tattagaaaa attcatccag
     2221 cagacgataa aacgcaatac gctggctatc cggtgccgca atgccataca gcaccagaaa
     2281 acgatccgcc cattcgccgc ccagttcttc cgcaatatca cgggtggcca gcgcaatatc
     2341 ctgataacga tccgccacgc ccagacggcc gcaatcaata aagccgctaa aacggccatt
     2401 ttccaccata atgttcggca ggcacgcatc accatgggtc accaccagat cttcgccatc
     2461 cggcatgctc gctttcagac gcgcaaacag ctctgccggt gccaggccct gatgttcttc
     2521 atccagatca tcctgatcca ccaggcccgc ttccatacgg gtacgcgcac gttcaatacg
     2581 atgtttcgcc tgatgatcaa acggacaggt cgccgggtcc agggtatgca gacgacgcat
     2641 ggcatccgcc ataatgctca ctttttctgc cggcgccaga tggctagaca gcagatcctg
     2701 acccggcact tcgcccagca gcagccaatc acggcccgct tcggtcacca catccagcac
     2761 cgccgcacac ggaacaccgg tggtggccag ccagctcaga cgcgccgctt catcctgcag
     2821 ctcgttcagc gcaccgctca gatcggtttt cacaaacagc accggacgac cctgcgcgct
     2881 cagacgaaac accgccgcat cagagcagcc aatggtctgc tgcgcccaat catagccaaa
     2941 cagacgttcc acccacgctg ccgggctacc cgcatgcagg ccatcctgtt caatcatact
     3001 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3061 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3121 gccac
//
