LOCUS       sequence-247993-        8862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  wild-type FOS with C-terminal V5 tag expressed from pLX vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Shao DD, Xue W, Krall EB, Bhutkar A, Piccioni F, Wang X, Schinzel 
            AC, Sood S, Rosenbluh J, Kim JW, Zwang Y, Roberts TM, Root DE, Jacks
            T, Hahn WC
  TITLE     KRAS and YAP1 Converge to Regulate EMT and Tumor Survival.
  JOURNAL   Cell. 2014 Jul 3;158(1):171-84. doi: 10.1016/j.cell.2014.06.004. 
            Epub 2014 Jun 19.
  PUBMED    24954536
REFERENCE   2  (bases 1 to 8862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.06.004"; journalName: "Cell"; date: "2014-07-3-
            3"; volume: "158"; issue: "1"; pages: "171-84"
COMMENT     Sequence Label: pLX304-FOS-V5
FEATURES             Location/Qualifiers
     source          1..8862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    607..631
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1772..1796)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1798..1839
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1881..2469
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1934..1954)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2352..2363)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2541..2774
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2852..2973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2889..2908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2943..2962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3013..3148
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3168..3187)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3169..3187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3197..3214)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3197..3213)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3206..3228)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3355..3810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3442..3461)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3652..3673
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3836..3940
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3941..4009,4010..4801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4159..4178)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4972..5560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5461..5480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5714..5731
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5848..5869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5884..5889,5890..5907,5908..5914)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5922..5938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5927..5949
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5946..5962
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5946..5962
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5981..6001
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5983..6001
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6029..6255
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6256..6436
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6483..6608
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7101..7334
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7519..7563
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7738..8238
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8157..8175
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8250..8645
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8705..8822
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggg ggcccggggt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactgtcg
      601 ggatcaacaa gtttgtacaa aaaagttggc atgatgttct cgggcttcaa cgcagactac
      661 gaggcgtcat cctcccgctg cagcagcgcg tccccggccg gggatagcct ctcttactac
      721 cactcacccg cagactcctt ctccagcatg ggctcgcctg tcaacgcgca ggacttctgc
      781 acggacctgg ccgtctccag tgccaacttc attcccacgg tcactgccat ctcgaccagt
      841 ccggacctgc agtggctggt gcagcccgcc ctcgtctcct ctgtggcccc atcgcagacc
      901 agagcccctc accctttcgg agtccccgcc ccctccgctg gggcttactc cagggctggc
      961 gttgtgaaga ccatgacagg aggccgagcg cagagcattg gcaggagggg caaggtggaa
     1021 cagttatctc cagaagaaga agagaaaagg agaatccgaa gggaaaggaa taagatggct
     1081 gcagccaaat gccgcaaccg gaggagggag ctgactgata cactccaagc ggagacagac
     1141 caactagaag atgagaagtc tgctttgcag accgagattg ccaacctgct gaaggagaag
     1201 gaaaaactag agttcatcct ggcagctcac cgacctgcct gcaagatccc tgatgacctg
     1261 ggcttcccag aagagatgtc tgtggcttcc cttgatctga ctgggggcct gccagaggtt
     1321 gccaccccgg agtctgagga ggccttcacc ctgcctctcc tcaatgaccc tgagcccaag
     1381 ccctcagtgg aacctgtcaa gagcatcagc agcatggagc tgaagaccga gccctttgat
     1441 gacttcctgt tcccagcatc atccaggccc agtggctctg agacagcccg ctccgtgcca
     1501 gacatggacc tatctgggtc cttctatgca gcagactggg agcctctgca cagtggctcc
     1561 ctggggatgg ggcccatggc cacagagctg gagcccctgt gcactccggt ggtcacctgt
     1621 actcccagct gcactgctta cacgtcttcc ttcgtcttca cctaccccga ggctgactcc
     1681 ttccccagct gtgcagctgc ccaccgcaag ggcagcagca gcaatgagcc ttcctctgac
     1741 tcgctcagct cacccacgct gctggccctg tgcccaactt tcttgtacaa agtggttggt
     1801 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac
     1861 cggtggcgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     1921 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     1981 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2041 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2101 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2161 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2221 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     2281 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     2341 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     2401 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     2461 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     2521 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     2581 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2641 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2701 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2761 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     2821 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     2881 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2941 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     3001 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3061 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3121 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     3181 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     3241 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     3301 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     3361 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3421 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3481 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3541 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3601 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3661 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3721 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3781 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     3841 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3901 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3961 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4021 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4081 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4141 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4201 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4261 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4321 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4381 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4441 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4501 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4561 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4621 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4681 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4741 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4801 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4861 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4921 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4981 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5041 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5101 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5161 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5221 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5281 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5341 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5401 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5461 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5521 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5581 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5641 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5701 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5761 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5821 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5881 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5941 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     6001 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     6061 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     6121 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     6181 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     6241 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6301 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6361 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6421 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     6481 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     6541 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     6601 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     6661 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     6721 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     6781 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     6841 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     6901 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     6961 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     7021 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     7081 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     7141 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     7201 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     7261 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     7321 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     7381 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     7441 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     7501 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     7561 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     7621 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     7681 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccttg
     7741 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
     7801 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
     7861 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
     7921 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt
     7981 ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga
     8041 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg
     8101 gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt
     8161 tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
     8221 accgacctct ctccccaggg ggtaccacca tggccaagcc tttgtctcaa gaagaatcca
     8281 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     8341 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     8401 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     8461 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     8521 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     8581 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     8641 agggcctgca gctgcagtag taagaattct agatcttgag acaaatggca gtattcatcc
     8701 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     8761 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     8821 ttcgggttta ttacagggac agcagagatc cactttggcg cc
//
