LOCUS       sequence-247997-        3245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-Lux-D codon opt. Nt chloroplasts
FEATURES             Location/Qualifiers
     source          1..3245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1451..1468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1561..2149)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1641..1660)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2323..3117)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(3118..3222)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatg gtctcaaatg gaaaatactc aacattcttt acctattgat catgtaattg
      421 atattggaga taatagatat attagagtat gggaaactaa acctaaaaat aaagaaacta
      481 aaagaaataa tactattgta attgcttctg gatttgctag aagaatggat cattttgctg
      541 gattagctga atatttagct aataatggat ttagagtaat tagatatgat tctttaaatc
      601 atgtaggatt atcttctgga gaaattaaac aattttctat gtctgtagga aaacattctt
      661 tattaactgt aattgattgg ttaaaagaaa gaaatattaa taatattgga ttaattgctt
      721 cttctttatc tgctagaatt gcttatgaag tagctgctga aattgattta tcttttttaa
      781 ttactgctgt aggagtagta aatttaagat ctactttaga aaaagcttta aaatatgatt
      841 atttacaaat ggaagtaaat actattcctg aagatttaat ttttgaagga cataatttag
      901 gatctaaagt atttgtaact gattgttttg aaaataattg ggattcttta gattctacta
      961 ttaataaaat ttgtgaatta gatattcctt ttattgcttt tacttctgat ggagatgatt
     1021 gggtatgtca acatgaagta aaacatttag tatctaatgt aaaatctgat aaaaaaaaaa
     1081 tttattcttt agtaggatct tctcatgatt taggagaaaa tttagtagta ttaagaaatt
     1141 tttatcaatc tatgactaaa gctgctgtat ctttagatag acaattagta gaattagtag
     1201 atgaaattat tgaacctaat tttgaagatt taactgtaat tactgtaaat gaaagaagat
     1261 taaaaaataa aattgaaaat gaaattatta atagattagc tgatagagta ttagcttctg
     1321 tataagcttt gagaccctgg gcctcatggg ccttcctttc actgcccgct ttccagtcgg
     1381 gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc
     1441 tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc
     1501 ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1981 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2101 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2161 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2221 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2281 atcaatctaa agtatatatg agtaaacttg gtctgacagt tattagaaaa attcatccag
     2341 cagacgataa aacgcaatac gctggctatc cggtgccgca atgccataca gcaccagaaa
     2401 acgatccgcc cattcgccgc ccagttcttc cgcaatatca cgggtggcca gcgcaatatc
     2461 ctgataacga tccgccacgc ccagacggcc gcaatcaata aagccgctaa aacggccatt
     2521 ttccaccata atgttcggca ggcacgcatc accatgggtc accaccagat cttcgccatc
     2581 cggcatgctc gctttcagac gcgcaaacag ctctgccggt gccaggccct gatgttcttc
     2641 atccagatca tcctgatcca ccaggcccgc ttccatacgg gtacgcgcac gttcaatacg
     2701 atgtttcgcc tgatgatcaa acggacaggt cgccgggtcc agggtatgca gacgacgcat
     2761 ggcatccgcc ataatgctca ctttttctgc cggcgccaga tggctagaca gcagatcctg
     2821 acccggcact tcgcccagca gcagccaatc acggcccgct tcggtcacca catccagcac
     2881 cgccgcacac ggaacaccgg tggtggccag ccagctcaga cgcgccgctt catcctgcag
     2941 ctcgttcagc gcaccgctca gatcggtttt cacaaacagc accggacgac cctgcgcgct
     3001 cagacgaaac accgccgcat cagagcagcc aatggtctgc tgcgcccaat catagccaaa
     3061 cagacgttcc acccacgctg ccgggctacc cgcatgcagg ccatcctgtt caatcatact
     3121 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3181 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3241 gccac
//
