LOCUS       sequence-247999-        9195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows for constitutive BMP7 expression from the murine ROSA26 safe 
            harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Routh ED, Pullikuth AK, Jin G, Chifman J, Chou JW, D'Agostino RB Jr,
            Seino KI, Wada H, Print CG, Zhang W, Lu Y, Miller LD
  TITLE     Transcriptomic Features of T Cell-Barren Tumors Are Conserved Across
            Diverse Tumor Types.
  JOURNAL   Front Immunol. 2020 Feb 13;11:57. doi: 10.3389/fimmu.2020.00057. 
            eCollection 2020.
  PUBMED    32117236
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2020-02-13"; volume: "11:57. doi"; pages: " 
            10.3389/fimmu.2020.00057. eCollection 2020"
COMMENT     Sequence Label: pR26-CMVconst-mBMP7
FEATURES             Location/Qualifiers
     source          1..9195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..794
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(813..878,879..916)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     polyA_signal    931..979
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1004..1305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1306..1508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1459..1479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2864..2912
                     /note="synthetic polyadenylation signal"
     primer_bind     3957..3976
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4135..4734
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVESAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4135..4154)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4631..4651
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4735..4856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4772..4791)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4826..4845
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    4913..5723
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(5797..5816)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5798..5816)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5823..5840)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5823..5839)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5832..5854)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5977..6279
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6158..6177
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6628..7422
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6628..7422
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6682..6701)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7292..7311
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      7622..7631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7628..8002
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      8140..8728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8629..8648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8882..8899
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9016..9037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9052..9057,9058..9075,9076..9082)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9090..9106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9095..9117
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9114..9130
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9114..9130
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9148..9166
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9148..9165
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cagttaacgg cagccggagt gcgcagccgc cggcagcctc gctctgccca ctgggtgggg
       61 cgggaggtag gtggggtgag gcgagctgga cgtgcgggcg cggtcggcct ctggcggggc
      121 gggggggggg agggagggtc agcgaaagta gctcgcgcgc gagcggccgc ccaccctccc
      181 cttcctctgg gggagtcgtt ttacccgccg ccggccgggc ctcgtcgtct gattggctct
      241 cggggcccag aaaactggcc cttgccattg gctcgtgttc gtgcaagttg agtccatccg
      301 ccggccagcg ggggcggcga ggaggcgctc ccaggttccg gccctcccct cggccccgcg
      361 ccgcagagtc tggccgcgcg cccctgcgca acgtggcagg aagcgcgcgc tgggggcggg
      421 gacgggcagt agggctgagc ggctgcgggg cgggtgcaag cacgtttccg acttgagttg
      481 cctcaagagg ggcgtgctga gccagacctc catcgcgcac tccggggagt ggagggaagg
      541 agcgagggct cagttgggct gttttggagg caggaagcac ttgctctccc aaagtcgctc
      601 tgagttgtta tcagtaaggg agctgcagtg gagtaggcgg ggagaaggcc gcacccttct
      661 ccggaggggg gaggggagtg ttgcaatacc tttctgggag ttctctgctg cctcctggct
      721 tctgaggacc gccctgggcc tgggagaatc ccttccccct cttccctcgt gatctgcaac
      781 tccagtcttt ctagaatcga tctcgagaac tgtagggcgc agtagtccag ggtttccttg
      841 atgatgtcat acttatcctg tccctttttt ttccacagct cgcggttgag gacaaactct
      901 tcgcggtctt tccagtggtt aattagggat cggcaataaa aagacagaat aaaacgcacg
      961 ggtgttgggt cgtttgttca tcgatgccct ttcgtcttca ttccgttaca taacttacgg
     1021 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1081 atgttcccat agtaacgcca atagggactt tccattgacg tcaatggtgg agtatttacg
     1141 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1201 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1261 tcctacttgg cagtacatct actattagtc atcgctatta ccatggtgat gcggttttgg
     1321 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccaccca
     1381 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1441 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1501 gcagagcttg gagaattcga gctcggtacc cggggatgct agcgaggaga tctgccacca
     1561 tgcacgtgcg ctcgctgcgc gctgcggcgc cacacagctt cgtggcgctc tgggcgcctc
     1621 tgttcttgct gcgctccgcc ctggccgatt tcagcctgga caacgaggtg cactccagct
     1681 tcatccaccg gcgcctccgc agccaggagc ggcgggagat gcagcgggag atcctgtcca
     1741 tcttagggtt gccccatcgc ccgcgcccgc acctccaggg aaagcataat tcggcgccca
     1801 tgttcatgtt ggacctgtac aacgccatgg cggtggagga gagcgggccg gacggacagg
     1861 gcttctccta cccctacaag gccgtcttca gtacccaggg ccccccttta gccagcctgc
     1921 aggacagcca cttcctcact gacgccgaca tggtcatgag cttcgtcaac ctagtggaac
     1981 atgacaaaga attcttccac cctcgatacc accatcggga gttccggttt gatctttcca
     2041 agatccccga gggcgaagcg gtgaccgcag ccgaattcag gatctataag gactacatcc
     2101 gggagcgatt tgacaacgag accttccaga tcacagtcta tcaggtgctc caggagcact
     2161 caggcaggga gtcggacctc ttcttgctgg acagccgcac catctgggct tctgaggagg
     2221 gctggttggt gtttgatatc acagccacca gcaaccactg ggtggtcaac cctcggcaca
     2281 acctgggctt acagctctct gtggagaccc tggatgggca gagcatcaac cccaagttgg
     2341 caggcctgat tggacggcat ggaccccaga acaagcaacc cttcatggtg gccttcttca
     2401 aggccacgga agtccatctc cgtagtatcc ggtccacggg gggcaagcag cgcagccaga
     2461 atcgctccaa gacgccaaag aaccaagagg ccctgaggat ggccagtgtg gcagaaaaca
     2521 gcagcagtga ccagaggcag gcctgcaaga aacatgagct gtacgtcagc ttccgagacc
     2581 ttggctggca ggactggatc attgcacctg aaggctatgc tgcctactac tgtgagggag
     2641 agtgcgcctt ccctctgaac tcctacatga acgccaccaa ccacgccatc gtccagacac
     2701 tggttcactt catcaaccca gacacagtac ccaagccctg ctgtgcgccc acccagctca
     2761 acgccatctc tgtcctctac ttcgacgaca gctctaatgt catcctgaag aagtacagaa
     2821 acatggtggt ccgggcctgt ggctgccact agacgcgtta ggaaataaaa gatctttatt
     2881 ttcattagat ctgtgtgttg gttttttgtg tgggcgcgcc gggttttggc gcctcccgcg
     2941 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
     3001 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
     3061 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
     3121 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag
     3181 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag
     3241 ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt
     3301 cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg
     3361 gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt
     3421 tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa
     3481 tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg
     3541 cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag
     3601 atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg
     3661 gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac
     3721 ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc
     3781 agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg
     3841 ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg
     3901 ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa
     3961 gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc
     4021 accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt
     4081 gtccgctaaa ttctggccgt ttttggcttt tttgttagac caagtttggc caccatgacc
     4141 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     4201 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     4261 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     4321 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     4381 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     4441 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     4501 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     4561 gtcgtgctcc ccggagtgga gtcggccgag cgcgccgggg tgcccgcctt cctggagacc
     4621 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     4681 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaacttg
     4741 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4801 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttagggt
     4861 accgagctcg gatccactag taacggccgc cagtgtgctg gaattcgccc ttagatgggc
     4921 gggagtcttc tgggcaggct taaaggctaa cctggtgtgt gggcgttgtc ctgcagggga
     4981 attgaacagg tgtaaaattg gagggacaag acttcccaca gattttcggt tttgtcggga
     5041 agttttttaa taggggcaaa taaggaaaat gggaggatag gtagtcatct ggggttttat
     5101 gcagcaaaac tacaggttat tattgcttgt gatccgcctc ggagtatttt ccatcgaggt
     5161 agattaaaga catgctcacc cgagttttat actctcctgc ttgagatcct tactacagta
     5221 tgaaattaca gtgtcgcgag ttagactatg taagcagaat tttaatcatt tttaaagagc
     5281 ccagtacttc atatccattt ctcccgctcc ttctgcagcc ttatcaaaag gtattttaga
     5341 acactcattt tagccccatt ttcatttatt atactggctt atccaacccc tagacagagc
     5401 attggcattt tccctttcct gatcttagaa gtctgatgac tcatgaaacc agacagatta
     5461 gttacataca ccacaaatcg aggctgtagc tggggcctca acactgcagt tcttttataa
     5521 ctccttagta cactttttgt tgatcctttg ccttgatcct taattttcag tgtctatcac
     5581 ctctcccgtc aggtggtgtt ccacatttgg gcctattctc agtccaggga gttttacaac
     5641 aatagatgta ttgagaatcc aacctaaagc ttaactttcc actcccatga atgcctctct
     5701 cctttttctc catttataaa ctgaagggcg aattctgcag atatccatca cactggcggc
     5761 cgctcgatcg agcatgcatc tagagggccc aattcgccct atagtgagtc gtattacaat
     5821 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5881 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5941 cgcccttccc aacagttgcg cagcctatac gtacggcagt ttaaggttta cacctataaa
     6001 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgccgggg
     6061 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     6121 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     6181 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     6241 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggcat gagattatca
     6301 aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa acggtgctga
     6361 ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga
     6421 aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca
     6481 gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa
     6541 gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc aagctctgat
     6601 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     6661 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     6721 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     6781 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     6841 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     6901 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     6961 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     7021 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     7081 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     7141 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     7201 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     7261 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     7321 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     7381 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattattaa cgcttacaat
     7441 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catcaggtgg
     7501 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7561 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatagc acgtgaggag
     7621 ggccaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     7681 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     7741 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     7801 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     7861 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     7921 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     7981 cgtggccgag gagcaggact gacacgtgct aaaacttcat ttttaattta aaaggatcta
     8041 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8281 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8761 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8821 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8881 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8941 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     9001 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     9061 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     9121 cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac tcaagctatg
     9181 catcaagctt ggtac
//
