LOCUS       sequence-24800-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Groth AC, Olivares EC, Thyagarajan B, Calos MP
  TITLE     A phage integrase directs efficient site-specific integration in 
            human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 May 23. 97(11):5995-6000.
  PUBMED    10801973
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 May 23. 97(11):5995-6000."
COMMENT     Sequence Label: pTA-attB
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    250..319
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(417..436)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(418..436)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(443..460)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(443..459)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(452..474)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(687..706)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gactggncga gctggatcca ctagtaacgg ccgccagtgt gctggaattc ggcttgtcga
       61 catgcccgcc gtgaccgtcg agaacccgct gacgctgccc cgcgtatccg cacccgccga
      121 cgccgtcgca cgtcccgtgc tcaccgtgac caccgcgccc agcggtttcg agggcgaggg
      181 cttcccggtg cgccgcgcgt tcgccgggat caactaccgc cacctcgacc cgttcatcat
      241 gatggaccag atgggtgagg tggagtacgc gcccggggag cccaagggca cgccctggca
      301 cccgcaccgc ggcttcgaga ccgtgaccta catcgtcgac ggtacctgaa gccgaattct
      361 gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc aattcgccct
      421 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      481 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      541 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
      601 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      661 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      721 gttcgccggc tttcccgtca agctctaatc gtgggctcct tnaggntncg attagtgctt
      781 acgcactcga cccaaaantt gataggtgat gttcagtatg ggcncgcntg aaangttttc
      841 nccttga
//
