LOCUS       sequence-248000-        3395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-Lux-A codon opt. Nt chloroplasts
FEATURES             Location/Qualifiers
     source          1..3395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1601..1618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1711..2299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1791..1810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2473..3267)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(3268..3372)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccgc ggtaccggtc tcaaatgaaa atttctaata tttgtttttc
      421 ttatcaacct cctggagaat ctcatcaaga agtaatggaa agatttatta gattaggagt
      481 agcttctgaa gaattaaatt ttgatggatt ttatacttta gaacatcatt ttactgaatt
      541 tggaattact ggaaatttat atattgcttg tgctaatatt ttaggaagaa ctaaaagaat
      601 tcaagtagga actatgggaa ttgtattacc tactgaacat cctgctagac atgtagaatc
      661 tttattagta ttagatcaat tatctaaagg aagatttaat tatggaactg taagaggatt
      721 atatcataaa gattttagag tatttggaac ttctcaagaa gattctagaa aaactgctga
      781 aaatttttat tctatgattt tagatgcttc taaaactgga gtattacata ctgatggaga
      841 agtagtagaa tttcctgatg taaatgtata tcctgaagct tattctaaaa aacaacctac
      901 ttgtatgact gctgaatctt ctgaaactat tacttattta gctgaaagag gattacctat
      961 ggtattatct tggattattc ctgtatctga aaaagtatct caaatggaat tatataatga
     1021 agtagctgct gaacatggac atgatattaa taatattgaa catattttaa cttttatttg
     1081 ttctgtaaat gaagatggag aaaaagctga ttctgtatgt agaaattttt tagaaaattg
     1141 gtatgattct tataaaaatg ctactaatat ttttaatgat tctaatcaaa ctagaggata
     1201 tgattattta aaagctcaat ggagagaatg ggtaatgaaa ggattagctg atcctagaag
     1261 aagattagat tattctaatg aattaaatcc tgtaggaact cctgaaagat gtattgaaat
     1321 tattcaatct aatattgatg ctactggaat taaacatatt actgtaggat ttgaagctaa
     1381 tggatctgaa caagaaatta gagaatctat ggaattattt atggaaaaag tagctcctca
     1441 tttaaaagat cctcaataag ctttgagacc gagctcggag cacaagactg gcctcatggg
     1501 ccttcctttc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaacatg
     1561 gtcatagctg tttccttgcg tattgggcgc tctccgcttc ctcgctcact gactcgctgc
     1621 gctcggtcgt tcgggtaaag cctggggtgc ctaatgagca aaaggccagc aaaaggccag
     1681 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1741 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1801 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1861 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1921 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1981 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2041 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2101 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2161 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2221 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2281 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2341 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2401 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2461 gtctgacagt tattagaaaa attcatccag cagacgataa aacgcaatac gctggctatc
     2521 cggtgccgca atgccataca gcaccagaaa acgatccgcc cattcgccgc ccagttcttc
     2581 cgcaatatca cgggtggcca gcgcaatatc ctgataacga tccgccacgc ccagacggcc
     2641 gcaatcaata aagccgctaa aacggccatt ttccaccata atgttcggca ggcacgcatc
     2701 accatgggtc accaccagat cttcgccatc cggcatgctc gctttcagac gcgcaaacag
     2761 ctctgccggt gccaggccct gatgttcttc atccagatca tcctgatcca ccaggcccgc
     2821 ttccatacgg gtacgcgcac gttcaatacg atgtttcgcc tgatgatcaa acggacaggt
     2881 cgccgggtcc agggtatgca gacgacgcat ggcatccgcc ataatgctca ctttttctgc
     2941 cggcgccaga tggctagaca gcagatcctg acccggcact tcgcccagca gcagccaatc
     3001 acggcccgct tcggtcacca catccagcac cgccgcacac ggaacaccgg tggtggccag
     3061 ccagctcaga cgcgccgctt catcctgcag ctcgttcagc gcaccgctca gatcggtttt
     3121 cacaaacagc accggacgac cctgcgcgct cagacgaaac accgccgcat cagagcagcc
     3181 aatggtctgc tgcgcccaat catagccaaa cagacgttcc acccacgctg ccgggctacc
     3241 cgcatgcagg ccatcctgtt caatcatact cttccttttt caatattatt gaagcattta
     3301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3361 aggggttccg cgcacatttc cccgaaaagt gccac
//
