LOCUS       sequence-248001-        3278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-Lux-B codon opt. Nt chloroplasts
FEATURES             Location/Qualifiers
     source          1..3278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1484..1501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1594..2182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1674..1693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2356..3150)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(3151..3255)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatg gtctcaaatg aattttggat tatttttttt aaattttcaa cctgaaggaa
      421 tgacttctga aatggtatta gataatatgg tagatactgt agctttagta gataaagatg
      481 attatcattt taaaagagta ttagtatctg aacatcattt ttctaaaaat ggaattattg
      541 gagaaccttt aactgctatt tcttttttat taggattaac taaaagaatt gaaattggat
      601 ctttaaatca agtaattact actcatcatc ctgtaagaat tggagaacaa actggattat
      661 tagatcaaat gtcttatgga agatttgtat taggattatc tgattgtgta aatgattttg
      721 aaatggattt ttttaaaaga aaaagatctt ctcaacaaca acaatttgaa gcttgttatg
      781 aaattttaaa tgaagcttta actactaatt attgtcaagc tgatgatgat ttttttaatt
      841 ttcctagaat ttctgtaaat cctcattgta tttctgaagt aaaacaatat attttagctt
      901 cttctatggg agtagtagaa tgggctgcta gaaaaggatt acctttaact tatagatggt
      961 ctgattcttt agctgaaaaa gaaaaatatt atcaaagata tttagctgta gctaaagaaa
     1021 ataatattga tgtatctaat attgatcatc aatttccttt attagtaaat attaatgaaa
     1081 atagaagaat tgctagagat gaagtaagag aatatattca atcttatgta tctgaagctt
     1141 atcctactga tcctaatatt gaattaagag tagaagaatt aattgaacaa catgctgtag
     1201 gaaaagtaga tgaatattat gattctacta tgcatgctgt aaaagtaact ggatctaaaa
     1261 atttattatt atcttttgaa tctatgaaaa ataaagatga tgtaactaaa ttaattaata
     1321 tgtttaatca aaaaattaaa gataatttaa ttaaataagc tttgagaccc tgggcctcat
     1381 gggccttcct ttcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaac
     1441 atggtcatag ctgtttcctt gcgtattggg cgctctccgc ttcctcgctc actgactcgc
     1501 tgcgctcggt cgttcgggta aagcctgggg tgcctaatga gcaaaaggcc agcaaaaggc
     1561 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1621 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1681 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1741 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1801 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1861 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1921 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1981 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2041 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2101 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2161 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2221 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2281 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2341 ttggtctgac agttattaga aaaattcatc cagcagacga taaaacgcaa tacgctggct
     2401 atccggtgcc gcaatgccat acagcaccag aaaacgatcc gcccattcgc cgcccagttc
     2461 ttccgcaata tcacgggtgg ccagcgcaat atcctgataa cgatccgcca cgcccagacg
     2521 gccgcaatca ataaagccgc taaaacggcc attttccacc ataatgttcg gcaggcacgc
     2581 atcaccatgg gtcaccacca gatcttcgcc atccggcatg ctcgctttca gacgcgcaaa
     2641 cagctctgcc ggtgccaggc cctgatgttc ttcatccaga tcatcctgat ccaccaggcc
     2701 cgcttccata cgggtacgcg cacgttcaat acgatgtttc gcctgatgat caaacggaca
     2761 ggtcgccggg tccagggtat gcagacgacg catggcatcc gccataatgc tcactttttc
     2821 tgccggcgcc agatggctag acagcagatc ctgacccggc acttcgccca gcagcagcca
     2881 atcacggccc gcttcggtca ccacatccag caccgccgca cacggaacac cggtggtggc
     2941 cagccagctc agacgcgccg cttcatcctg cagctcgttc agcgcaccgc tcagatcggt
     3001 tttcacaaac agcaccggac gaccctgcgc gctcagacga aacaccgccg catcagagca
     3061 gccaatggtc tgctgcgccc aatcatagcc aaacagacgt tccacccacg ctgccgggct
     3121 acccgcatgc aggccatcct gttcaatcat actcttcctt tttcaatatt attgaagcat
     3181 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3241 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
