LOCUS       sequence-248003-        3031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-Lux-G codon opt. Nt chloroplasts
FEATURES             Location/Qualifiers
     source          1..3031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1237..1254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1347..1935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1427..1446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2109..2903)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2904..3008)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggcctaggc gcgccatgag ctcggtctca aatgattttt aattgtaaag taaaaaaagt
      421 agaagcttct gattctcata tttataaagt atttattaaa cctgataaat gttttgattt
      481 taaagctgga caatatgtaa ttgtatattt aaatggaaaa aatttacctt tttctattgc
      541 taattgtcct acttgtaatg aattattaga attacatgta ggaggatctg taaaagaatc
      601 tgctattgaa gctatttctc attttattaa tgcttttatt tatcaaaaag aatttactat
      661 tgatgctcct catggagatg cttggttaag agatgaatct caatctcctt tattattaat
      721 tgctggagga actggattat cttatattaa ttctatttta tcttgttgta tttctaaaca
      781 attatctcaa cctatttatt tatattgggg agtaaataat tgtaatttat tatatgctga
      841 tcaacaatta aaaactttag ctgctcaata tagaaatatt aattatattc ctgtagtaga
      901 aaatttaaat actgattggc aaggaaaaat tggaaatgta attgatgctg taattgaaga
      961 tttttctgat ttatctgatt ttgatattta tgtatgtgga ccttttggaa tgtctagaac
     1021 tgctaaagat attttaattt ctcaaaaaaa agctaatatt ggaaaaatgt attctgatgc
     1081 tttttcttat acttaagctt tgagaccggt acctcttaat taactggcct catgggcctt
     1141 cctttcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aacatggtca
     1201 tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg ctcactgact cgctgcgctc
     1261 ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa ggccaggaac
     1321 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1381 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1441 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1501 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1561 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1621 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1681 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1741 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1801 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1861 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1921 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1981 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2041 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2101 gacagttatt agaaaaattc atccagcaga cgataaaacg caatacgctg gctatccggt
     2161 gccgcaatgc catacagcac cagaaaacga tccgcccatt cgccgcccag ttcttccgca
     2221 atatcacggg tggccagcgc aatatcctga taacgatccg ccacgcccag acggccgcaa
     2281 tcaataaagc cgctaaaacg gccattttcc accataatgt tcggcaggca cgcatcacca
     2341 tgggtcacca ccagatcttc gccatccggc atgctcgctt tcagacgcgc aaacagctct
     2401 gccggtgcca ggccctgatg ttcttcatcc agatcatcct gatccaccag gcccgcttcc
     2461 atacgggtac gcgcacgttc aatacgatgt ttcgcctgat gatcaaacgg acaggtcgcc
     2521 gggtccaggg tatgcagacg acgcatggca tccgccataa tgctcacttt ttctgccggc
     2581 gccagatggc tagacagcag atcctgaccc ggcacttcgc ccagcagcag ccaatcacgg
     2641 cccgcttcgg tcaccacatc cagcaccgcc gcacacggaa caccggtggt ggccagccag
     2701 ctcagacgcg ccgcttcatc ctgcagctcg ttcagcgcac cgctcagatc ggttttcaca
     2761 aacagcaccg gacgaccctg cgcgctcaga cgaaacaccg ccgcatcaga gcagccaatg
     2821 gtctgctgcg cccaatcata gccaaacaga cgttccaccc acgctgccgg gctacccgca
     2881 tgcaggccat cctgttcaat catactcttc ctttttcaat attattgaag catttatcag
     2941 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3001 gttccgcgca catttccccg aaaagtgcca c
//
