LOCUS       sequence-24801-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Groth AC, Olivares EC, Thyagarajan B, Calos MP
  TITLE     A phage integrase directs efficient site-specific integration in 
            human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 May 23. 97(11):5995-6000.
  PUBMED    10801973
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 May 23. 97(11):5995-6000."
COMMENT     Sequence Label: pTA-attB
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    158..227
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(325..344)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(326..344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(351..368)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(351..367)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(360..382)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(595..614)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agctactccc gcgtatcgca cccgccgacg ccgtcgcacg tcccgtgctc accgtgacca
       61 ccgcgcccag cggtttcgag ggcgagggct tcccggtgcg ccgcgcgttc gccgggatca
      121 actaccgcca cctcgacccg ttcatcatga tggaccagat gggtgaggtg gagtacgcgc
      181 ccggggagcc caagggcacg ccctggcacc cgcaccgcgg cttcgagacc gtgacctaca
      241 tcgtcgacgg tacctgaagc cgaattctgc agatatccat cacactggcg gccgctcgag
      301 catgcatcta gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
      361 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      421 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      481 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
      541 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      601 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      661 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      721 attagggtga tggttcaggt anggggccat cgcctnaata nacggttttn cnccntttga
      781 cgttggantt c
//
