LOCUS       sequence-248011-        7940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows for constitutive gene expression from the murine ROSA26 safe 
            harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Routh ED, Pullikuth AK, Jin G, Chifman J, Chou JW, D'Agostino RB Jr,
            Seino KI, Wada H, Print CG, Zhang W, Lu Y, Miller LD
  TITLE     Transcriptomic Features of T Cell-Barren Tumors Are Conserved Across
            Diverse Tumor Types.
  JOURNAL   Front Immunol. 2020 Feb 13;11:57. doi: 10.3389/fimmu.2020.00057. 
            eCollection 2020.
  PUBMED    32117236
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2020-02-13"; volume: "11:57. doi"; pages: " 
            10.3389/fimmu.2020.00057. eCollection 2020"
COMMENT     Sequence Label: pR26-CMVconst
FEATURES             Location/Qualifiers
     source          1..7940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..794
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(813..878,879..916)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     polyA_signal    931..979
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1004..1305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1306..1508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1459..1479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1609..1657
                     /note="synthetic polyadenylation signal"
     primer_bind     2702..2721
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             2880..3479
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVESAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2880..2899)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3376..3396
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3480..3601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3517..3536)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3571..3590
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    3658..4468
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(4542..4561)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4543..4561)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4568..4585)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4568..4584)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4577..4599)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             4722..5024
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4903..4922
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             5373..6167
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5373..6167
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5427..5446)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6037..6056
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      6367..6376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6373..6747
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      6885..7473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7374..7393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7627..7644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7761..7782
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7797..7802,7803..7820,7821..7827)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7835..7851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7840..7862
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7859..7875
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7859..7875
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7893..7911
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7893..7910
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cagttaacgg cagccggagt gcgcagccgc cggcagcctc gctctgccca ctgggtgggg
       61 cgggaggtag gtggggtgag gcgagctgga cgtgcgggcg cggtcggcct ctggcggggc
      121 gggggagggg agggagggtc agcgaaagta gctcgcgcgc gagcggccgc ccaccctccc
      181 cttcctctgg gggagtcgtt ttacccgccg ccggccgggc ctcgtcgtct gattggctct
      241 cggggcccag aaaactggcc cttgccattg gctcgtgttc gtgcaagttg agtccatccg
      301 ccggccagcg ggggcggcga ggaggcgctc ccaggttccg gccctcccct cggccccgcg
      361 ccgcagagtc tggccgcgcg cccctgcgca acgtggcagg aagcgcgcgc tgggggcggg
      421 gacgggcagt agggctgagc ggctgcgggg cgggtgcaag cacgtttccg acttgagttg
      481 cctcaagagg ggcgtgctga gccagacctc catcgcgcac tccggggagt ggagggaagg
      541 agcgagggct cagttgggct gttttggagg caggaagcac ttgctctccc aaagtcgctc
      601 tgagttgtta tcagtaaggg agctgcagtg gagtaggcgg ggagaaggcc gcacccttct
      661 ccggaggggg gaggggagtg ttgcaatacc tttctgggag ttctctgctg cctcctggct
      721 tctgaggacc gccctgggcc tgggagaatc ccttccccct cttccctcgt gatctgcaac
      781 tccagtcttt ctagaatcga tctcgagaac tgtagggcgc agtagtccag ggtttccttg
      841 atgatgtcat acttatcctg tccctttttt ttccacagct cgcggttgag gacaaactct
      901 tcgcggtctt tccagtggtt aattagggat cggcaataaa aagacagaat aaaacgcacg
      961 ggtgttgggt cgtttgttca tcgatgccct ttcgtcttca ttccgttaca taacttacgg
     1021 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1081 atgttcccat agtaacgcca atagggactt tccattgacg tcaatggtgg agtatttacg
     1141 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1201 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1261 tcctacttgg cagtacatct actattagtc atcgctatta ccatggtgat gcggttttgg
     1321 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccaccca
     1381 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1441 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1501 gcagagcttg gagaattcga gctcggtacc cggggatgct agctctaggt taattaagcc
     1561 cgggccctgc aggtaggatg aatttaaatg tttaaacacg cgttaggaaa taaaagatct
     1621 ttattttcat tagatctgtg tgttggtttt ttgtgtgggc gcgccgggtt ttggcgcctc
     1681 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
     1741 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
     1801 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
     1861 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
     1921 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     1981 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
     2041 atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc
     2101 gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc
     2161 aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc
     2221 ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg
     2281 gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg
     2341 gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga
     2401 actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc
     2461 cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat
     2521 aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg
     2581 cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg
     2641 gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag
     2701 ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt
     2761 taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt
     2821 aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagaccaagt ttggccacca
     2881 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     2941 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     3001 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     3061 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     3121 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     3181 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     3241 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     3301 gcgccgtcgt gctccccgga gtggagtcgg ccgagcgcgc cggggtgccc gccttcctgg
     3361 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     3421 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa
     3481 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3541 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3601 agggtaccga gctcggatcc actagtaacg gccgccagtg tgctggaatt cgcccttaga
     3661 tgggcgggag tcttctgggc aggcttaaag gctaacctgg tgtgtgggcg ttgtcctgca
     3721 ggggaattga acaggtgtaa aattggaggg acaagacttc ccacagattt tcggttttgt
     3781 cgggaagttt tttaataggg gcaaataagg aaaatgggag gataggtagt catctggggt
     3841 tttatgcagc aaaactacag gttattattg cttgtgatcc gcctcggagt attttccatc
     3901 gaggtagatt aaagacatgc tcacccgagt tttatactct cctgcttgag atccttacta
     3961 cagtatgaaa ttacagtgtc gcgagttaga ctatgtaagc agaattttaa tcatttttaa
     4021 agagcccagt acttcatatc catttctccc gctccttctg cagccttatc aaaaggtatt
     4081 ttagaacact cattttagcc ccattttcat ttattatact ggcttatcca acccctagac
     4141 agagcattgg cattttccct ttcctgatct tagaagtctg atgactcatg aaaccagaca
     4201 gattagttac atacaccaca aatcgaggct gtagctgggg cctcaacact gcagttcttt
     4261 tataactcct tagtacactt tttgttgatc ctttgccttg atccttaatt ttcagtgtct
     4321 atcacctctc ccgtcaggtg gtgttccaca tttgggccta ttctcagtcc agggagtttt
     4381 acaacaatag atgtattgag aatccaacct aaagcttaac tttccactcc catgaatgcc
     4441 tctctccttt ttctccattt ataaactgaa gggcgaattc tgcagatatc catcacactg
     4501 gcggccgctc gatcgagcat gcatctagag ggcccaattc gccctatagt gagtcgtatt
     4561 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4621 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4681 ccgatcgccc ttcccaacag ttgcgcagcc tatacgtacg gcagtttaag gtttacacct
     4741 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     4801 cggggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
     4861 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     4921 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     4981 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggcatgagat
     5041 tatcaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt
     5101 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     5161 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     5221 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     5281 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagct
     5341 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     5401 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5461 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5521 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     5581 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     5641 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5701 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     5761 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     5821 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5881 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5941 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6001 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6061 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6121 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt attaacgctt
     6181 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatca
     6241 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6301 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atagcacgtg
     6361 aggagggcca ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     6421 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     6481 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     6541 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     6601 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     6661 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     6721 cacttcgtgg ccgaggagca ggactgacac gtgctaaaac ttcattttta atttaaaagg
     6781 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6841 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6901 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6961 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7021 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7081 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7141 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7201 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7261 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7321 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7381 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7441 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7501 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7561 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7621 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7681 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     7741 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7801 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7861 ggaaacagct atgaccatga ttacgccaag ctatttaggt gacactatag aatactcaag
     7921 ctatgcatca agcttggtac
//
