LOCUS       sequence-248013-        3849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter acceptor vector. Minimal cargo vector; with eGFP without 
            promoter in downstream secondary site. Allows introducing any part 
            using BsmBI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-Pac
FEATURES             Location/Qualifiers
     source          1..3849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    82..102
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             130..150
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             170..877
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             170..877
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(194..222)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     817..837
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      917..1011
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1084..1175
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1176..1244,1245..2036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2040..2079
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2226..2334
                     /note="incP origin of transfer"
     rep_origin      2370..2958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2859..2878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3112..3129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3144..3284
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3210..3229)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3370..3392
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3386..3577)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3757..3843)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtcttgaga cgtctctgac ctactagtag cggccgctgc agggagtaac ttctgactga
      121 gttgcatact tgtttaactt taagaaggag atatacaatg gtagcccgta aaggagaaga
      181 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      241 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
      301 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta
      361 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
      421 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
      481 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
      541 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
      601 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
      661 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
      721 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
      781 cctttcgaaa gatcccaacg aaaagagaga tcacatggtc cttcttgagt ttgtaacagc
      841 tgctgggatt acacatggca tggatgaact atacaaaggt tcgtaacttc tgactgagtt
      901 gcacgctcta gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
      961 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc
     1021 cccaataatt acgatttaaa ttagtagccc gcctaatgag cgggcttttt tttaattccc
     1081 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1141 gataaatgct tcaataatat tgaaaaagga agagtatgag cattcagcat tttcgtgtgg
     1201 cgctgattcc gttttttgcg gcgttttgcc tgccggtgtt tgcgcatccg gaaaccctgg
     1261 tgaaagtgaa agatgcggaa gatcaactgg gtgcgcgcgt gggctatatt gaactggatc
     1321 tgaacagcgg caaaattctg gaatcttttc gtccggaaga acgttttccg atgatgagca
     1381 cctttaaagt gctgctgtgc ggtgcggttc tgagccgtgt ggatgcgggc caggaacaac
     1441 tgggccgtcg tattcattat agccagaacg atctggtgga atatagcccg gtgaccgaaa
     1501 aacatctgac cgatggcatg accgtgcgtg aactgtgcag cgcggcgatt accatgagcg
     1561 ataacaccgc ggcgaacctg ctgctgacga ccattggcgg tccgaaagaa ctgaccgcgt
     1621 ttctgcataa catgggcgat catgtgaccc gtctggatcg ttgggaaccg gaactgaacg
     1681 aagcgattcc gaacgatgaa cgtgatacca ccatgccggc agcaatggcg accaccctgc
     1741 gtaaactgct gacgggtgag ctgctgaccc tggcaagccg ccagcaactg attgattgga
     1801 tggaagcgga taaagtggcg ggtccgctgc tgcgtagcgc gctgccggct ggctggttta
     1861 ttgcggataa aagcggtgcg ggcgaacgtg gcagccgtgg cattattgcg gcgctgggcc
     1921 cggatggtaa accgagccgt attgtggtga tttataccac cggcagccag gcgacgatgg
     1981 atgaacgtaa ccgtcagatt gcggaaattg gcgcgagcct gattaaacat tggtaaaccg
     2041 atacaattaa aggctccttt tggagccttt ttttttggac gacccttgtc cttttccgct
     2101 gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca
     2161 cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt
     2221 cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc
     2281 ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc
     2341 ggccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2401 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2461 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     2521 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2581 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2641 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2701 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2761 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2821 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2881 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2941 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3001 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3061 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3121 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     3181 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3241 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     3301 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3361 tgacagtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3421 ggcagctgcg gtaaagctca tcagcgtggt cgtgcagcga ttcacagatg tctgcctgtt
     3481 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
     3541 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
     3601 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
     3661 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
     3721 tgcggcgggg gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt
     3781 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     3841 ttgatgcct
//
