LOCUS       sequence-248015-        3482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Universal Acceptor Plasmid. The fusion sites of the basic part 
            generated are introduced during domestication. sfGFP reporter gene. 
            High copy number oriV #8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 101 to 135)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-Uac
FEATURES             Location/Qualifiers
     source          1..3482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        101..135
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             140..160
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             177..887
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    952..972
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1006..1100
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1173..1264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1265..1333,1334..2125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2129..2168
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2315..2423
                     /note="incP origin of transfer"
     rep_origin      2459..3047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2948..2967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3201..3218
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3335..3356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3390..3476)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctt gagacgactg tgacaaggag ttgacggcta gctcagtcct
      121 aggtacagtg ctagcgtact tgtttaactt taagaaggag atatacaatg gtagcccgta
      181 aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca
      241 acggtcataa gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga
      301 cgctgaagtt catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga
      361 cgctgactta tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact
      421 tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg
      481 acggcacgta caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca
      541 ttgagctgaa aggcattgac tttaaagaag atggcaatat cctgggccat aagctggaat
      601 acaattttaa cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag
      661 cgaattttaa aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc
      721 agcaaaacac tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca
      781 cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt
      841 tcgtaaccgc agcgggcatc acgcatggta tggatgaact gtacaaaggt tcgtaacttc
      901 tgactgagtt gcacgctcct tggtcagcgt ctcagacctt tcatcgcgac ctactagtag
      961 cggccgctgc agggagttgt cttcgaagac ttcgctctag tcttggactc ctgttgatag
     1021 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     1081 cgttttttat tggtgagaat ccaggggtcc ccaataatta cgatttaaat tagtagcccg
     1141 cctaatgagc gggctttttt ttaattcccc tatttgttta tttttctaaa tacattcaaa
     1201 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     1261 gagtatgagc attcagcatt ttcgtgtggc gctgattccg ttttttgcgg cgttttgcct
     1321 gccggtgttt gcgcatccgg aaaccctggt gaaagtgaaa gatgcggaag atcaactggg
     1381 tgcgcgcgtg ggctatattg aactggatct gaacagcggc aaaattctgg aatcttttcg
     1441 tccggaagaa cgttttccga tgatgagcac ctttaaagtg ctgctgtgcg gtgcggttct
     1501 gagccgtgtg gatgcgggcc aggaacaact gggccgtcgt attcattata gccagaacga
     1561 tctggtggaa tatagcccgg tgaccgaaaa acatctgacc gatggcatga ccgtgcgtga
     1621 actgtgcagc gcggcgatta ccatgagcga taacaccgcg gcgaacctgc tgctgacgac
     1681 cattggcggt ccgaaagaac tgaccgcgtt tctgcataac atgggcgatc atgtgacccg
     1741 tctggatcgt tgggaaccgg aactgaacga agcgattccg aacgatgaac gtgataccac
     1801 catgccggca gcaatggcga ccaccctgcg taaactgctg acgggtgagc tgctgaccct
     1861 ggcaagccgc cagcaactga ttgattggat ggaagcggat aaagtggcgg gtccgctgct
     1921 gcgtagcgcg ctgccggctg gctggtttat tgcggataaa agcggtgcgg gcgaacgtgg
     1981 cagccgtggc attattgcgg cgctgggccc ggatggtaaa ccgagccgta ttgtggtgat
     2041 ttataccacc ggcagccagg cgacgatgga tgaacgtaac cgtcagattg cggaaattgg
     2101 cgcgagcctg attaaacatt ggtaaaccga tacaattaaa ggctcctttt ggagcctttt
     2161 tttttggacg acccttgtcc ttttccgctg cataaccctg cttcggggtc attatagcga
     2221 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
     2281 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
     2341 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     2401 atcctgctct gcgaggctgg ccgtaggccg gccccgtaga aaagatcaaa ggatcttctt
     2461 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2521 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2581 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2641 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2701 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2761 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2821 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2881 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2941 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3001 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3061 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3121 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3181 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3241 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3301 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     3361 caggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac
     3421 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
     3481 ct
//
