LOCUS       sequence-248030-        4399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - 
            Pseudomonas shuttle).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 91 to 125)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP24-1A(sfGFP)
FEATURES             Location/Qualifiers
     source          1..4399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        91..125
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             130..150
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             167..877
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    930..950
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      984..1078
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1199..1218)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1210..2025
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2192..2300
                     /note="incP origin of transfer"
     rep_origin      2394..2982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2883..2902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3036..3869)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3883..4234
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4307..4393)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgccttgga acacctgctt ttcgctgaat tcgcggccgc
       61 ttctagagcg tctctggagt gagaccggag ttgacggcta gctcagtcct aggtacagtg
      121 ctagcgtact tgtttaactt taagaaggag atatacaatg gtagcccgta aaggcgaaga
      181 gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa
      241 gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt
      301 catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta
      361 tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc
      421 cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta
      481 caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa
      541 aggcattgac tttaaagaag atggcaatat cctgggccat aagctggaat acaattttaa
      601 cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa
      661 aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac
      721 tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt
      781 tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc
      841 agcgggcatc acgcatggta tggatgaact gtacaaaggt tcgtaacttc tgactgagtt
      901 gcacgctggt ctctcgctaa tgtgagacgt actagtagcg gccgctgcag ggagttgtct
      961 tcgaagactt cgctctagtc ttggactcct gttgatagat ccagtaatga cctcagaact
     1021 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
     1081 aggggtcccc aataattacg atttaaattt gtgtctcaaa atctctgatg ttacattgca
     1141 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1201 aggggtgtta tgagccatat tcagcgtgaa acgagctgta gccgtccgcg tctgaacagc
     1261 aacatggatg cggatctgta tggctataaa tgggcgcgtg ataacgtggg tcagagcggc
     1321 gcgaccattt atcgtctgta tggcaaaccg gatgcgccgg aactgtttct gaaacatggc
     1381 aaaggcagcg tggcgaacga tgtgaccgat gaaatggtgc gtctgaactg gctgaccgaa
     1441 tttatgccgc tgccgaccat taaacatttt attcgcaccc cggatgatgc gtggctgctg
     1501 accaccgcga ttccgggcaa aaccgcgttt caggtgctgg aagaatatcc ggatagcggc
     1561 gaaaacattg tggatgcgct ggccgtgttt ctgcgtcgtc tgcatagcat tccggtgtgc
     1621 aactgcccgt ttaacagcga tcgtgtgttt cgtctggccc aggcgcagag ccgtatgaac
     1681 aacggcctgg tggatgcgag cgattttgat gatgaacgta acggctggcc ggtggaacag
     1741 gtgtggaaag aaatgcataa actgctgccg tttagcccgg atagcgtggt gacccacggc
     1801 gattttagcc tggataacct gattttcgat gaaggcaaac tgattggctg cattgatgtg
     1861 ggccgtgtgg gcattgcgga tcgttatcag gatctggcca ttctgtggaa ctgcctgggc
     1921 gaatttagcc cgagcctgca aaaacgtctg tttcagaaat atggcattga taatccggat
     1981 atgaacaaac tgcaatttca tctgatgctg gatgaatttt tctaataatt aattggaccg
     2041 cggtccgcgc gttgtccttt tccgctgcat aaccctgctt cggggtcatt atagcgattt
     2101 tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag
     2161 actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg
     2221 cgagcgggtg ttccttcttc actgtccctt attcgcacat ggcggtgctc aacgggaatc
     2281 ctgctctgcg aggctggccg taggccggcc gataatctca tgaccaaaat cccttaacgt
     2341 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2401 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2461 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2521 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2581 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2641 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2701 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2761 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2821 gcggacaggc atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2881 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2941 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3001 gtgaaaggca ggccggtccg tggtggccac ggcctctagg ccagatccag cggcatctgg
     3061 gttagtcgag cgcgggccgc ttcccatgtc tcaccagggc gagcctgttt cgcgatctca
     3121 gcatctgaaa tcttcccggc cttgcgcttc gctggggcct tacccaccgc cttggcgggc
     3181 ttcttcggtc caaaactgaa caacagatgt gtgaccttgc gcccggtctt tcgctgcgcc
     3241 cactccacct gtagcgggct gtgctcgttg atctgcgtca cggctggatc aagcactcgc
     3301 aacttgaagt ccttgatcga gggataccgg ccttccagtt gaaaccactt tcgcagctgg
     3361 tcaatttcta tttcgcgctg gccgatgctg tcccattgca tgagcagctc gtaaagcctg
     3421 atcgcgtggg tgctgtccat cttggccacg tcagccaagg cgtatttggt gaactgtttg
     3481 gtgagttccg tcaggtacgg cagcatgtct ttggtgaacc tgagttctac acggccctca
     3541 ccctcccggt agatgattgt ttgcacccag ccggtaatca tcacactcgg tcttttcccc
     3601 ttgccattgg gctcttgggt taaccggact tcccgccgtt tcaggcgcag ggccgcttct
     3661 ttgagctggt tgtaggaaga ttcgataggg acacccgcca tcgtcgctat gtcctccgcc
     3721 gtcactgaat acatcacttc atcggtgaca ggctcgctcc tcttcacctg gctaatacag
     3781 gccagaacga tccgctgttc ctgaacactg aggcgatacg cggcctcgac cagggcattg
     3841 cttttgtaaa ccattggggg tgaggccacg ttcgacattc cttgtgtata aggggacact
     3901 gtatctgcgt cccacaatac aacaaatccg tccctttaca acaacaaatc cgtcccttct
     3961 taacaacaaa tccgtccctt aatggcaaca aatccgtccc tttttaaact ctacaggcca
     4021 cggattacgt ggcctgtaga cgtcctaaaa ggtttaaaag ggaaaaggaa gaaaagggtg
     4081 gaaacgcaaa aaacgcacca ctacgtggcc ccgttggggc cgcatttgtg cccctgaagg
     4141 ggcgggggag gcgtctgggc aatccccgtt ttaccagtcc cctatcgccg cctgagaggg
     4201 cgcaggaagc gagtaatcag ggtatcgagg cggattcacc cttggcgtcc aaccagcggc
     4261 accagcggcg cctgagaggg gcgcgcccag ctgtctaggg cggcggattt gtcctactca
     4321 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
     4381 ttcgttttat ttgatgcct
//
