LOCUS       sequence-248045-        2523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-TMV 3'UTR
FEATURES             Location/Qualifiers
     source          1..2523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(729..746)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(839..1427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(919..938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1601..2395)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2396..2500)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct cagcttataa taaataacgg attgtgtccg
      421 taatcacacg tggtgcgtac gataacgcat agtgtttttc cctccactta aatcgaaggg
      481 ttgtgtcttg gatcgcgcgg gtcaaatgta tatggttcat atacatccgc aggcacgtaa
      541 taaagcgagg ggttcgaatc cccccgttac ccccggtagg ggcccacgct tgagaccggt
      601 acctggagca caagactggc ctcatgggcc ttcctttcac tgcccgcttt ccagtcggga
      661 aacctgtcgt gccagctgca ttaacatggt catagctgtt tccttgcgta ttgggcgctc
      721 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct
      781 aatgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      841 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      901 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      961 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1021 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1081 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1141 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1201 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1261 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1321 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1381 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1441 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1501 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1561 caatctaaag tatatatgag taaacttggt ctgacagtta ttagaaaaat tcatccagca
     1621 gacgataaaa cgcaatacgc tggctatccg gtgccgcaat gccatacagc accagaaaac
     1681 gatccgccca ttcgccgccc agttcttccg caatatcacg ggtggccagc gcaatatcct
     1741 gataacgatc cgccacgccc agacggccgc aatcaataaa gccgctaaaa cggccatttt
     1801 ccaccataat gttcggcagg cacgcatcac catgggtcac caccagatct tcgccatccg
     1861 gcatgctcgc tttcagacgc gcaaacagct ctgccggtgc caggccctga tgttcttcat
     1921 ccagatcatc ctgatccacc aggcccgctt ccatacgggt acgcgcacgt tcaatacgat
     1981 gtttcgcctg atgatcaaac ggacaggtcg ccgggtccag ggtatgcaga cgacgcatgg
     2041 catccgccat aatgctcact ttttctgccg gcgccagatg gctagacagc agatcctgac
     2101 ccggcacttc gcccagcagc agccaatcac ggcccgcttc ggtcaccaca tccagcaccg
     2161 ccgcacacgg aacaccggtg gtggccagcc agctcagacg cgccgcttca tcctgcagct
     2221 cgttcagcgc accgctcaga tcggttttca caaacagcac cggacgaccc tgcgcgctca
     2281 gacgaaacac cgccgcatca gagcagccaa tggtctgctg cgcccaatca tagccaaaca
     2341 gacgttccac ccacgctgcc gggctacccg catgcaggcc atcctgttca atcatactct
     2401 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2461 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2521 cac
//
