LOCUS       sequence-248047-        2446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-BMV 3'UTR
FEATURES             Location/Qualifiers
     source          1..2446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(652..669)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(762..1350)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(842..861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1524..2318)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2319..2423)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggcctaggc gcgccaggtc tcagcttggt gcctttgaga gtctactttt gctctcttcg
      421 gaagaaccct taggggttcg tgcatgggct tgcatagcaa gtctagatgc gggtaccgta
      481 cagtgttgaa aaacactgta aatctctaaa agaaaccacg cttgagacca ttaattaact
      541 ggcctcatgg gccttccttt cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      601 gcattaacat ggtcatagct gtttccttgc gtattgggcg ctctccgctt cctcgctcac
      661 tgactcgctg cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag
      721 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      781 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      841 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      901 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      961 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1021 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1081 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1141 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1201 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1261 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1321 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1381 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1441 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1501 gagtaaactt ggtctgacag ttattagaaa aattcatcca gcagacgata aaacgcaata
     1561 cgctggctat ccggtgccgc aatgccatac agcaccagaa aacgatccgc ccattcgccg
     1621 cccagttctt ccgcaatatc acgggtggcc agcgcaatat cctgataacg atccgccacg
     1681 cccagacggc cgcaatcaat aaagccgcta aaacggccat tttccaccat aatgttcggc
     1741 aggcacgcat caccatgggt caccaccaga tcttcgccat ccggcatgct cgctttcaga
     1801 cgcgcaaaca gctctgccgg tgccaggccc tgatgttctt catccagatc atcctgatcc
     1861 accaggcccg cttccatacg ggtacgcgca cgttcaatac gatgtttcgc ctgatgatca
     1921 aacggacagg tcgccgggtc cagggtatgc agacgacgca tggcatccgc cataatgctc
     1981 actttttctg ccggcgccag atggctagac agcagatcct gacccggcac ttcgcccagc
     2041 agcagccaat cacggcccgc ttcggtcacc acatccagca ccgccgcaca cggaacaccg
     2101 gtggtggcca gccagctcag acgcgccgct tcatcctgca gctcgttcag cgcaccgctc
     2161 agatcggttt tcacaaacag caccggacga ccctgcgcgc tcagacgaaa caccgccgca
     2221 tcagagcagc caatggtctg ctgcgcccaa tcatagccaa acagacgttc cacccacgct
     2281 gccgggctac ccgcatgcag gccatcctgt tcaatcatac tcttcctttt tcaatattat
     2341 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
