LOCUS       sequence-248048-        2484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-ALMV 3'UTR
FEATURES             Location/Qualifiers
     source          1..2484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(690..707)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(800..1388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(880..899)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1562..2356)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2357..2461)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcagctt cgttttcttg acataagtca aattgccaac
      421 ctccactggg tggattaagg ttgaggtata gaatcctatt cgctcctgat aggagaaatt
      481 ctatattgct tatatatgtg cttatgcaca tatataaatg ctcatgcaaa actgcatgaa
      541 tgcccctaag ggatgccgct tgagaccggt accctctggg cctcatgggc cttcctttca
      601 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaacatgg tcatagctgt
      661 ttccttgcgt attgggcgct ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      721 cgggtaaagc ctggggtgcc taatgagcaa aaggccagca aaaggccagg aaccgtaaaa
      781 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      841 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      901 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      961 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1021 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1081 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1141 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1201 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1261 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1321 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1381 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1441 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1501 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1561 attagaaaaa ttcatccagc agacgataaa acgcaatacg ctggctatcc ggtgccgcaa
     1621 tgccatacag caccagaaaa cgatccgccc attcgccgcc cagttcttcc gcaatatcac
     1681 gggtggccag cgcaatatcc tgataacgat ccgccacgcc cagacggccg caatcaataa
     1741 agccgctaaa acggccattt tccaccataa tgttcggcag gcacgcatca ccatgggtca
     1801 ccaccagatc ttcgccatcc ggcatgctcg ctttcagacg cgcaaacagc tctgccggtg
     1861 ccaggccctg atgttcttca tccagatcat cctgatccac caggcccgct tccatacggg
     1921 tacgcgcacg ttcaatacga tgtttcgcct gatgatcaaa cggacaggtc gccgggtcca
     1981 gggtatgcag acgacgcatg gcatccgcca taatgctcac tttttctgcc ggcgccagat
     2041 ggctagacag cagatcctga cccggcactt cgcccagcag cagccaatca cggcccgctt
     2101 cggtcaccac atccagcacc gccgcacacg gaacaccggt ggtggccagc cagctcagac
     2161 gcgccgcttc atcctgcagc tcgttcagcg caccgctcag atcggttttc acaaacagca
     2221 ccggacgacc ctgcgcgctc agacgaaaca ccgccgcatc agagcagcca atggtctgct
     2281 gcgcccaatc atagccaaac agacgttcca cccacgctgc cgggctaccc gcatgcaggc
     2341 catcctgttc aatcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2401 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2461 gcacatttcc ccgaaaagtg ccac
//
