LOCUS       sequence-248061-        3446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 vector. With lacZ as alternative cloning reporter. 
            Thermosensitive origin pSC101 (low copy number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 91 to 125)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP27ts-1A(lacZ)
FEATURES             Location/Qualifiers
     source          1..3446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        91..125
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             130..150
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    486..506
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      540..634
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(755..774)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             766..1581
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1748..1856
                     /note="incP origin of transfer"
     CDS             complement(1867..2817)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSIRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2865..3087
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3354..3440)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgccttgga acacctgctt ttcgctgaat tcgcggccgc
       61 ttctagagcg tctctggagt gagaccggag ttgacggcta gctcagtcct aggtacagtg
      121 ctagcgtact tgtttaactt taagaaggag atatacaatg gtagccggtg acccgaaact
      181 gctgctggaa gttccgcacg ctatctcttc ttctccgggt aactctctgg ctgttgttct
      241 gcaacgtcgt gactgggaaa acccgggtgt tacccagctg aaccgtctgg ctgctcaccc
      301 gccgttcgct tcttggcgta actctgaaga agctcgtacc gaccgtccgt cccagcagct
      361 gcgttctctg atccgtctgc tgaccaaacc ggaacgtaaa ctgtcttggc tgctgccgcc
      421 gctgtctaac aacggttcgt aacttctgac tgagttgcac gctggtctct cgctaatgtg
      481 agacgtacta gtagcggccg ctgcagggag ttgtcttcga agacttcgct ctagtcttgg
      541 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
      601 cggttgccgc cgggcgtttt ttattggtga gaatccaggg gtccccaata attacgattt
      661 aaatttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
      721 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcag
      781 cgtgaaacga gctgtagccg tccgcgtctg aacagcaaca tggatgcgga tctgtatggc
      841 tataaatggg cgcgtgataa cgtgggtcag agcggcgcga ccatttatcg tctgtatggc
      901 aaaccggatg cgccggaact gtttctgaaa catggcaaag gcagcgtggc gaacgatgtg
      961 accgatgaaa tggtgcgtct gaactggctg accgaattta tgccgctgcc gaccattaaa
     1021 cattttattc gcaccccgga tgatgcgtgg ctgctgacca ccgcgattcc gggcaaaacc
     1081 gcgtttcagg tgctggaaga atatccggat agcggcgaaa acattgtgga tgcgctggcc
     1141 gtgtttctgc gtcgtctgca tagcattccg gtgtgcaact gcccgtttaa cagcgatcgt
     1201 gtgtttcgtc tggcccaggc gcagagccgt atgaacaacg gcctggtgga tgcgagcgat
     1261 tttgatgatg aacgtaacgg ctggccggtg gaacaggtgt ggaaagaaat gcataaactg
     1321 ctgccgttta gcccggatag cgtggtgacc cacggcgatt ttagcctgga taacctgatt
     1381 ttcgatgaag gcaaactgat tggctgcatt gatgtgggcc gtgtgggcat tgcggatcgt
     1441 tatcaggatc tggccattct gtggaactgc ctgggcgaat ttagcccgag cctgcaaaaa
     1501 cgtctgtttc agaaatatgg cattgataat ccggatatga acaaactgca atttcatctg
     1561 atgctggatg aatttttcta ataattaatt ggaccgcggt ccgcgcgttg tccttttccg
     1621 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     1681 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     1741 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     1801 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg
     1861 ccggcctcag atccttccgt atttagccag tatgttctct agtgtggttc gttgtttttg
     1921 cgtgagccat gagaacgaac cattgagatc atacttactt tgcatgtcac tcaaaaattt
     1981 tgcctcaaaa ctggtgagct gaatttttgc agttaaagca tcgtgtagtg tttttcttag
     2041 tccgttatgt aggtaggaat ctgatgtaat ggttgttggt attttgtcac cattcatttt
     2101 tatctggttg ttctcaagtt cggttacgag atccatttgt ctatctagtt caacttggaa
     2161 aatcaacgta tcagtcgggc ggcctcgctt atcaaccacc aatttcatat tgctgtaagt
     2221 gtttaaatct ttacttattg gtttcaaaac ccattggtta agccttttaa actcatggta
     2281 gttattttca agcattaaca tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt
     2341 gtgagttttc ttttgtgtta gttcttttaa taaccactca taaatcctaa tagagtattt
     2401 gttttcaaaa gacttaacat gttccagatt atattttatg aattttttta actggaaaag
     2461 ataaggcaat atctcttcac taaaaactaa ttctaatttt tcgcttgaga acttggcata
     2521 gtttgtccac tggaaaatct caaagccttt aaccaaagga ttcctgattt ccacagttct
     2581 cgtcatcagc tctctggttg ctttagctaa tacaccataa gcattttccc tactgatgtt
     2641 catcatctga acgtattggt tataagtgaa cgataccgtc cgttctttcc ttgtagggtt
     2701 ttcaatcgtg gggttgagta gtgccacaca gcataaaatt agcttggttt catgctccgt
     2761 taagtcatag cgactaatcg ctagttcatt tgctttgaaa acaactaatt cagacataca
     2821 tctcaattgg tctaggtgat tttaatcact ataccaattg agatgggcta gtcaatgata
     2881 attacatgtc cttttccttt gagttgtggg tatctgtaaa ttctgctaga cctttgctgg
     2941 aaaacttgta aattctgcta gaccctctgt aaattccgct agacctttgt gtgttttttt
     3001 tgtttatatt caagtggtta taatttatag aataaagaaa gaataaaaaa agataaaaag
     3061 aatagatccc agccctgtgt ataactcact actttagtca gttccgcagt attacaaaag
     3121 gatgtcgcaa acgctgtttg ctcctctaca aaacagacct taaaacccta aaggcttaag
     3181 tagcaccctc gcaagctcgg gcaaatcgct gaatattcct tttgtctccg accatcaggc
     3241 acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct ctggcagtga
     3301 atgggggtaa atggcactac aggcgcggcg cgcccagctg tctagggcgg cggatttgtc
     3361 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     3421 tgagcctttc gttttatttg atgcct
//
