LOCUS       sequence-248074-        4269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 vector. With lacZ as alternative cloning reporter. 
            Thermosensitive origin pSC101 (low copy number). Constitutive 
            mCherry in downstream secondary site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP27ts[mCherry]-1A(lacZ)
FEATURES             Location/Qualifiers
     source          1..4269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        91..125
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             130..150
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    486..506
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     promoter        511..545
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             550..570
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             587..1291
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(729..747)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      1312..1339
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1363..1457
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1578..1597)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1589..2404
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2571..2679
                     /note="incP origin of transfer"
     CDS             complement(2690..3640)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSIRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3688..3910
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4177..4263)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgccttgga acacctgctt ttcgctgaat tcgcggccgc
       61 ttctagagcg tctctggagt gagaccggag ttgacggcta gctcagtcct aggtacagtg
      121 ctagcgtact tgtttaactt taagaaggag atatacaatg gtagccggtg acccgaaact
      181 gctgctggaa gttccgcacg ctatctcttc ttctccgggt aactctctgg ctgttgttct
      241 gcaacgtcgt gactgggaaa acccgggtgt tacccagctg aaccgtctgg ctgctcaccc
      301 gccgttcgct tcttggcgta actctgaaga agctcgtacc gaccgtccgt cccagcagct
      361 gcgttctctg atccgtctgc tgaccaaacc ggaacgtaaa ctgtcttggc tgctgccgcc
      421 gctgtctaac aacggttcgt aacttctgac tgagttgcac gctggtctct cgctaatgtg
      481 agacgtacta gtagcggccg ctgcagggag ttgacggcta gctcagtcct aggtacagtg
      541 ctagcgtact tgtttaactt taagaaggag atatacaatg gtagccgtga gcaagggcga
      601 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      661 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      721 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      781 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      841 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      901 cggcggcgtg gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa
      961 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaaaaccat
     1021 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     1081 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     1141 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     1201 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     1261 ctccaccggc ggcatggacg agctgtacaa gggttcgtga tcgatcacac tggctcacct
     1321 tcgggtgggc ctttctgcgt ttatatgttc gctctagtct tggactcctg ttgatagatc
     1381 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
     1441 tttttattgg tgagaatcca ggggtcccca ataattacga tttaaatttg tgtctcaaaa
     1501 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     1561 ttacataaac agtaatacaa ggggtgttat gagccatatt cagcgtgaaa cgagctgtag
     1621 ccgtccgcgt ctgaacagca acatggatgc ggatctgtat ggctataaat gggcgcgtga
     1681 taacgtgggt cagagcggcg cgaccattta tcgtctgtat ggcaaaccgg atgcgccgga
     1741 actgtttctg aaacatggca aaggcagcgt ggcgaacgat gtgaccgatg aaatggtgcg
     1801 tctgaactgg ctgaccgaat ttatgccgct gccgaccatt aaacatttta ttcgcacccc
     1861 ggatgatgcg tggctgctga ccaccgcgat tccgggcaaa accgcgtttc aggtgctgga
     1921 agaatatccg gatagcggcg aaaacattgt ggatgcgctg gccgtgtttc tgcgtcgtct
     1981 gcatagcatt ccggtgtgca actgcccgtt taacagcgat cgtgtgtttc gtctggccca
     2041 ggcgcagagc cgtatgaaca acggcctggt ggatgcgagc gattttgatg atgaacgtaa
     2101 cggctggccg gtggaacagg tgtggaaaga aatgcataaa ctgctgccgt ttagcccgga
     2161 tagcgtggtg acccacggcg attttagcct ggataacctg attttcgatg aaggcaaact
     2221 gattggctgc attgatgtgg gccgtgtggg cattgcggat cgttatcagg atctggccat
     2281 tctgtggaac tgcctgggcg aatttagccc gagcctgcaa aaacgtctgt ttcagaaata
     2341 tggcattgat aatccggata tgaacaaact gcaatttcat ctgatgctgg atgaattttt
     2401 ctaataatta attggaccgc ggtccgcgcg ttgtcctttt ccgctgcata accctgcttc
     2461 ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt
     2521 tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata
     2581 ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg
     2641 gcggtgctca acgggaatcc tgctctgcga ggctggccgt aggccggcct cagatccttc
     2701 cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg
     2761 aaccattgag atcatactta ctttgcatgt cactcaaaaa ttttgcctca aaactggtga
     2821 gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta tgtaggtagg
     2881 aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg ttgttctcaa
     2941 gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg
     3001 ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa tctttactta
     3061 ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt tcaagcatta
     3121 acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt ttcttttgtg
     3181 ttagttcttt taataaccac tcataaatcc taatagagta tttgttttca aaagacttaa
     3241 catgttccag attatatttt atgaattttt ttaactggaa aagataaggc aatatctctt
     3301 cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc cactggaaaa
     3361 tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc agctctctgg
     3421 ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc tgaacgtatt
     3481 ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc gtggggttga
     3541 gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca tagcgactaa
     3601 tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat tggtctaggt
     3661 gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacat gtccttttcc
     3721 tttgagttgt gggtatctgt aaattctgct agacctttgc tggaaaactt gtaaattctg
     3781 ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat attcaagtgg
     3841 ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat cccagccctg
     3901 tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg caaacgctgt
     3961 ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct
     4021 cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt
     4081 tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac
     4141 tacaggcgcg gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt
     4201 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     4261 ttgatgcct
//
