LOCUS       sequence-248080-        6396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Abscisic acid-inducible B4GALT1 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Chang MM, Gaidukov L, Jung G, Tseng WA, Scarcelli JJ, Cornell R, 
            Marshall JK, Lyles JL, Sakorafas P, Chu AA, Cote K, Tzvetkova B, 
            Dolatshahi S, Sumit M, Mulukutla BC, Lauffenburger DA, Figueroa B 
            Jr, Summers NM, Lu TK, Weiss R
  TITLE     Small-molecule control of antibody N-glycosylation in engineered 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 
            10.1038/s41589-019-0288-4.
  PUBMED    31110306
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 
            10.1038/s41589-019-0288-4."
COMMENT     Sequence Label: B4GALT1-ABA
FEATURES             Location/Qualifiers
     source          1..6396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    570..595
                     /label=SA
                     /note="splice acceptor site"
     primer_bind     1007..1026
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1025..1080
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1126..1145
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     protein_bind    1235..1259
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1261..1659)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      1656..1665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1675..1699)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    1709..1746
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    complement(1764..1784)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1829..1848)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1830..1848)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1858..1875)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1858..1874)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1867..1889)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2016..2471
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2103..2122)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2313..2334
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2497..2601
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2602..2670,2671..3462)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2820..2839)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3633..4221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4122..4141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4474..5283)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5197..5216
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5275..5294
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    5404..6240
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
       61 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
      121 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
      181 acggggaaaa agctttaggc tgaaagagag atttagaatg acaggcgcgc ctgggagctc
      241 acggggacag ccccctccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg
      301 ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc atccccgagc
      361 cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc tttcctctga
      421 acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga aaaagcttta
      481 ggctgaaaga gagatttaga atgacaggcg cgccaactcg aattcattgc agactttcac
      541 tggtaccatc gatgcaattg tctcgaggcc ctgtgggagg aagagaagag gtcagaagct
      601 tggcacagtc aatgaagttt gaaaaccaat aagagctcgg taaacctagg gataacaggg
      661 taattctaga gaattcaagc ttgggctgca ggtcgaggga tctccataag agaagaggga
      721 cagctatgac tgggagtagt caggagagga ggaaaaatct ggctagtaaa acatgtaagg
      781 aaaattttag ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa aaaatctaac
      841 ctcaagtcaa ggcttttcta tggaataagg aatggacagc agggggctgt ttcatatact
      901 gatgacctct ttatagccaa cctttgttca tggcagccag catatgggca tatgttgcca
      961 aactctaaac caaatactca ttctgatgtt ttaaatgatt tgccctccca tatgtccttc
     1021 cgagtgagag acacaaaaaa ttccaacaca ctattgcaat gaaaataaat ttcctttatt
     1081 agccagaagt cagatgctca aggggcttca tgatgtcccc ataatttttg gcagagggaa
     1141 aaagatctca gtggtatttg tgagccaggg cattggccac accagccacc accttctgat
     1201 aggcagcctg cacctgagga gtgaattcac gcgtaccact ttgtacaaga aagctgggta
     1261 ttagccctcc cacacataac cagagggcag caattcacga atcccaactg ccgtcggctg
     1321 tccatcactg tccttcacta tggctttgat cccaggatgc agatcgagaa gcacctgtcg
     1381 gcaccgtccg caggggctca agatgcccct gttctcattt ccgatcgcga cgatacaagt
     1441 caggttgcca gctgccgcag cagcagcagt gcccagcacc acgagttctg cacaaggtcc
     1501 cccagtaaaa tgatatacat tgacaccagt gaagatgcgg ccgtcgctag agagagctgc
     1561 gctggcgacg ctgtagtctt cagagatggg gatgctgttg attgtagccg ttgctctttc
     1621 aatgagggtg gattcttctt gagacaaagg cttggccatg gtggcaggcc gttcagcctg
     1681 cttttttgta caaacttggg tcgaattcat gatcctgacg acggagtccg ccgtcgtcga
     1741 caagccctcg agaaattcgg agccaacttt tctatacaaa gttgatatca ctagcgttaa
     1801 cgtcgacatc gatacggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     1861 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     1921 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     1981 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     2041 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2101 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2161 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2221 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2281 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2341 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     2401 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     2461 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2521 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2581 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2641 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2701 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2761 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2821 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2881 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2941 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3001 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3061 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3121 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3181 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3241 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3301 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3361 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3421 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3481 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3541 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4261 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctcaattggg ttaggcgact
     4321 gttataactt acctctgtaa tactagtgat accgtctgac gctcagtgga acgacgcgta
     4381 actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca gcgtaatgct
     4441 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
     4501 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     4561 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     4621 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     4681 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
     4741 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     4801 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     4861 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     4921 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     4981 tccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     5041 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     5101 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     5161 caagcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     5221 taaatcagca tccatgttgg aatttaatcg cggcctcgac gtttcccgtt gaatatggct
     5281 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat
     5341 atttttatct tgtgcaatgt aacatcagag attttgagac acgggccaga gctcccgcgg
     5401 tggagagcag agccaggaac ccctgtaggg aaggggcagg agagccaggg gcatgagatg
     5461 gtggacgagg aagggggaca gggaagcctg agcgcctctc ctgggcttgc caaggactca
     5521 aacccagaag cccagagcag ggccttaggg aagcgggacc ctgctctggg cggaggaata
     5581 tgtcccagat agcactgggg actctttaag gaaagaagga tggagaaaga gaaagggagt
     5641 agaggcggcc acgacctggt gaacacctag gacgcaccat tctcacaaag ggagttttcc
     5701 acacggacac ccccctcctc accacagccc tgccaggacg gggctggcta ctggccttat
     5761 ctcacaggta aaactgacgc acggaggaac aatataaatt ggggactaga aaggtgaaga
     5821 gccaaagtta gaactcagga ccaacttatt ctgattttgt ttttccaaac tgcttctcct
     5881 cttgggaagt gtaaggaagc tgcagcacca ggatcagtga aacgcaccag acagccgcgt
     5941 cagagcagct caggttctgg gagagggtag cgcagggtgg ccactgagaa ccgggcaggt
     6001 cacgcatccc ccccttccct cccaccccct gccaagctct ccctcccagg atcctctctg
     6061 gctccatcgt aagcaaacct tagaggttct ggcaaggaga gagatggctc caggaaatgg
     6121 gggtgtgtca ccagataagg aatctgccta acaggaggtg ggggttagac ccaatatcag
     6181 gagactagga aggaggaggc ctaaggatgg ggcttttctg tcaccaatcc tgtccctagt
     6241 agcggccgat taccctgtta tccctaggat cttaaaaaca ttatacaata ctacaagcat
     6301 aaaaacgcac cgtcgacatc gatacggtac cgagttggcg cgcctgggag ctcacgggga
     6361 cagccccccc ccaaagcccc cagggatgta attacg
//
