LOCUS       sequence-248090-        6152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 83 to 117)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP45-2A(sfGFP)
FEATURES             Location/Qualifiers
     source          1..6152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        83..117
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             122..142
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..869
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    922..942
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      976..1070
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1306..2097
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            2243..2351
                     /note="incP origin of transfer"
     CDS             complement(2362..3213)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(3200..4039)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(4550..5521)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      5628..6022
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(6060..6146)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctctgga gtgagacggg agttgacggc tagctcagtc ctaggtacag tgctagcgta
      121 cttgtttaac tttaagaagg agatatacaa tggtagcccg taaaggcgaa gagctgttca
      181 ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg
      241 tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta
      301 ctactggtaa actgccggta ccttggccga ctctggtaac gacgctgact tatggtgttc
      361 agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc
      421 cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc
      481 gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg
      541 actttaaaga agatggcaat atcctgggcc ataagctgga atacaatttt aacagccaca
      601 atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc
      661 acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg
      721 gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta
      781 aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca
      841 tcacgcatgg tatggatgaa ctgtacaaag gttcgtaact tctgactgag ttgcacgctc
      901 gtctctcgct aatgtgagac ctactagtag cggccgctgc agggagttgt cttcgaagac
      961 ttcgctctag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
     1021 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
     1081 ccaataatta cgatttacgt atttaaatga accttgaccg aacgcagcgg tggtaacggc
     1141 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     1201 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     1261 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg
     1321 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     1381 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     1441 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     1501 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     1561 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     1621 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     1681 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     1741 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     1801 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggtga tgagcgaaat
     1861 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     1921 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     1981 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     2041 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataagac
     2101 aattgtcctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
     2161 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
     2221 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
     2281 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
     2341 gaggctggcc gtaggccggc ctcagcctgc cgccttgggc cgggtgatgt cgtacttgcc
     2401 cgccgcgaac tcggttaccg tccagcccag cgcgaccagc tccggcaacg cctcgcgcac
     2461 ccgctggcgg cgcttgcgca tggtcgaacc actggcctct gacggccaga catagccgca
     2521 caaggtatct atggaagcct tgccggtttt gccggggtcg atccagccac acagccgctg
     2581 gtgcagcagg cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc tgatgcgcac
     2641 atgctggccg ccacccatga cggcctgcgc gatcaagggg ttcagggcca cgtacaggcg
     2701 cccgtccgcc tcgtcgctgg cgtactccga cagcagccga aacccctgcc gcttgcggcc
     2761 attctgggct atgatggata ccttccaaag gcgctcgatg cagtcctgta tgtgcttgag
     2821 cgccccacca ctatcgacct ctgccccgat ttcctttgcc agcgcccgat agctaccttt
     2881 gaccaccatg gcatcagcgg tgacggcctc ccacttgggt tccaggaaca gccggagctg
     2941 ccgtccgcct tcggtcttgg gttccgggcc aagcactagg ccattaggcc cagccatggc
     3001 caccagccct tgcaggatgc gcagatcatc agcgcccagc ggctccgggc cgctgaactc
     3061 gatccgcttg ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct tgcgctcgcc
     3121 ccgcttgagg gcacggaaca ggccgggggc cagacagtgc gccgggtcgt gccggacgtg
     3181 gctgaggctg tgcttgttct taggcttcac cacggggcac ccccttgctc ttgcgctgcc
     3241 tctccagcac ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac cgatcagcga
     3301 acggtgcgcc atagttggcc ttgctcacac cgaagcggac gaagaaccgg cgctggtcgt
     3361 cgtccacacc ccattcctcg gcctcggcgc tggtcatgct cgacaggtag gactgccagc
     3421 ggatgttatc gaccagtacc gagctgcccc ggctggcctg ctgctggtcg cctgcgccca
     3481 tcatggccgc gcccttgctg gcatggtgca ggaacacgat agagcacccg gtatcggcgg
     3541 ctatggcctc catgcgaccg atgacctggg ccatggggcc gctggcgttt tcttcctcga
     3601 tgtggaaccg gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg gcgcgcttga
     3661 ggccgtcgaa ccactccggg gccatgatgt tgggcaggct gccgatcagc ggctggatca
     3721 gcaggccgtc agccacggct tgccgttcct cggcgctgag gtgcgcccca agggcgtgca
     3781 ggcggtgatg aatggcggtg ggcggatctt cggcgggcag gtagatcacc gggccggtgg
     3841 gcagttcgcc cacctccagc agatccggcc cgcctgcaat ctgtgcggcc agttgcaggg
     3901 ccagcatgga tttaccggca ccaccgggcg acaccagcgc cccgaccgta ccggccacca
     3961 tgttgggcaa aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc agaatattga
     4021 taggcttatg ggtagccatt gattgcctcc tttgcaggca gttggtggtt aggcgctggc
     4081 ggggtcacta cccccgccct gcgccgctct gagttcttcc aggcactcgc gcagcgcctc
     4141 gtattcgtcg tcggtcagcc agaacttgcg ctgacgcatc cctttggcct tcatgcgctc
     4201 ggcatatcgc gcttggcgta cagcgtcagg gctggccagc aggtcgccgg tctgcttgtc
     4261 cttttggtct ttcatatcag tcaccgagaa acttgccggg gccgaaaggc ttgtgttcgc
     4321 ggaacaagga caaggtgcag ccgtcaaggt taaggctggc catatcagcg actgaaaagc
     4381 ggccagcctc ggccttgttt gacgtataac caaagccacc gggcaaccaa tagcccttgt
     4441 cacttttgat caggtagacc gaccctgaag cgcttttttc gtattccata aaaccccctt
     4501 ctgtgcgtga gtactcatag tataacaggc gtgagtacca acgcaagcac tacatgctga
     4561 aatctggccc gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt gccagctcgg
     4621 cccgcgcaag ctggacgctg ggcagaccca tgaccttgct gacggtgcgc tcgatgtaat
     4681 ccgcttcgtg gccgggcttg cgctctgcca gcgctgggct ggcctcggcc atggccttgc
     4741 cgatttcctc ggcactgcgg ccccggctgg ccagcttctg cgcggcgata aagtcgcact
     4801 tgctgaggtc atcaccgaag cgcttgacca gcccggccat ctcgctgcgg tactcgtcca
     4861 gcgccgtgcg ccggtggcgg ctaagctgcc gctcgggcag ttcgaggctg gccagcctgc
     4921 gggccttctc ctgctgccgc tgggcctgct cgatctgctg gccagcctgc tgcaccagcg
     4981 ccgggccagc ggtggcggtc ttgcccttgg attcacgcag cagcacccac ggctgataac
     5041 cggcgcgggt ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg ccatagtggc
     5101 ggctgtcggc gctggccggg tcggcgtcgt actcgctggc cagcgtccgg gcaatctgcc
     5161 cccgaagttc accgcctgcg gcgtcggcca ccttgaccca tgcctgatag ttcttcgggc
     5221 tggtttccac taccagggca ggctcccggc cctcggcttt catgtcatcc aggtcaaact
     5281 cgctgaggtc gtccaccagc accagaccat gccgctcctg ctcggcgggc ctgatataca
     5341 cgtcattgcc ctgggcattc atccgcttga gccatggcgt gttctggagc acttcggcgg
     5401 ctgaccattc ccggttcatc atctggccgg tggtggcgtc cctgacgccg atatcgaagc
     5461 gctcacagcc catggccttg agctgtcggc ctatggcctg caaagtcctg tcgttcttca
     5521 tcgggccacc aagcgattcc cacacattat acgagccgga agcataaagt gtagctagat
     5581 ccgaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc tgcacacgcg
     5641 cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct ccagcgtatt
     5701 tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct gccgcgcagc
     5761 ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct ctggccgggc
     5821 atattgggca agggcagcag cgccccacaa gggcgctgat aaccgcgcct agtggattat
     5881 tcttagataa tcatggatgg atttttccaa caccccgcca gcccccgccc ctgctgggtt
     5941 tgcaggtttg ggggcgtgac agttattgca ggggttcgtg acagttattg caggggggcg
     6001 tgacagttat tgcaggggtt cgtgacagtt agggcgcgcc cagctgtcta gggcggcgga
     6061 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     6121 ttcgactgag cctttcgttt tatttgatgc ct
//
