LOCUS       sequence-24813-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Keravala A, Portlock JL, Nash JA, Vitrant DG, Robbins PD, Calos MP
  TITLE     PhiC31 integrase mediates integration in cultured synovial cells and
            enhances gene expression in rabbit joints.
  JOURNAL   J Gene Med. 2006 Aug . 8(8):1008-17.
  PUBMED    16779871
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene Med.
            2006 Aug . 8(8):1008-17."
COMMENT     Sequence Label: pDB2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..95
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    219..340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(256..275)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     310..329
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    549..618
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 ccaggnnntc tggcnggaga gctgtacagt ccggactcag atctcgagct caagcttcga
       61 attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa
      121 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      181 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
      241 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
      301 attctagttg tggtttgtcc aaactcatca atgtatctta acgcaattcg gcttgtcgac
      361 atgcccgccg tgaccgtcga gaacccgctg acgctgcccc gcgtatccgc acccgccgac
      421 gccgtcgcac gtcccgtgct caccgtgacc accgcgccca gcggtttcga gggcgagggc
      481 ttcccggtgc gccgcgcgtt cgccgggatc aactaccgcc acctcgaccc gttcatcatg
      541 atggaccaga tgggtgaggt ggagtacgcg cccggggagc ccaagggcac gccctggcac
      601 ccgcaccgcg gcttcgagac cgtgacctac atcgtcgacg gtacctgaag ccgaattcgc
      661 gtaaattgta agcgttaant tttngttaaa attcgcgtta aatttttgtt aaatcagctc
      721 attttaaaca taaggcgaaa tcgnnaaatc ctgtataatc aaagatagac gannaggttg
      781 atgtgtccan tggacaaatc cctntaaaag ngatcacnca ggggaannnc tnggnggnng
      841 ccctactgac
//
