LOCUS       sequence-248134-        7206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-2::gfp1-10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   He S, Cuentas-Condori A, Miller DM
  TITLE     NATF (Native And Tissue-Specific Fluorescence): A Strategy for 
            Bright, Tissue-Specific GFP Labeling of Native Proteins in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Apr 5. pii: genetics.119.302063. doi: 
            10.1534/genetics.119.302063.
  PUBMED    30952669
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Apr 5. pii: genetics.119.302063. doi: 
            10.1534/genetics.119.302063."
COMMENT     Sequence Label: pSH88
FEATURES             Location/Qualifiers
     source          1..7206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1420..2061
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     protein_bind    complement(2063..2087)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2857..2877)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2885..2902)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2885..2901)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2894..2916)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3229..3251
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3289..3307)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3375..3479
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3480..3548,3549..4340)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3698..3717)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4511..5099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5000..5019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5226..5248
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5245..5261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5245..5261
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atagatttag tgtggattta accgaaaaat gcagatcttt taaaaattta aaccagtgtt
       61 ttgttgcctt atttgcgaaa aaaaattgtg atatttcgag tattgctgca atttgattag
      121 taaaatgtta ggatataagt atttaaaaag tataattggt tgaatttcct ttttcatcac
      181 cgacttccaa aaatatagag atactgagtt ttcaccattt tttgaatgta aataaaaaca
      241 ttttgaacac ttgtgctggg attcaatcca aatacattca ctgtcattta atattattat
      301 atatcaaaat aaaacgcata actttttctg ttttttttta aattttttca aaatgtaggt
      361 ttggtaagtt aaggtaaggt taggcaatgt aggtaaacac gagatgatac tccacgttga
      421 aaattgtttt cataaataca gtttagattg aactgaagga ttttatcgtc taatagaata
      481 taatttttta gttcaagatt taggatttgt tttcactttt ttttagattc aagacatata
      541 tctcgcaaaa tgagttttgt ctttgagcca tcttgtcatc atagcacatt gaattttgtt
      601 gttcgcaaga cggaatatat gttcccagct gcaaattttg tgtcggatat ttcttcttta
      661 gcgaaaggcg ttaaaaacca aaaatagtcg aagcgtttga tatgtgtccc gttggttttt
      721 cgtgtccatc tattctgttt cgagtacaca tgttgcgacg cggcactgat gaaaaagtga
      781 aagagctagt gtcacacaca aacacaaagt atgctcatgt gagtgtgtag atatgtgtag
      841 atgtatgtcc tgaaggaata aaaagacttt ttgaagctac attttaaaca aaaaaagaca
      901 gaaacatgaa gcacaactct ctattctgtc gaacggcgtg tgcaactcac gcacacccaa
      961 aaatatgaaa tataacattc gcaccaacaa agcgcgaata tcaatgaatc tcgagggaac
     1021 gatggctgcc tgccggcgcg tgttgctctt ctgtcattgg gataactggc gggtctgttg
     1081 ggtccgtcct ttagaccatt tgactacact gccctgcata tgtgtgaaac gtcgtcgttg
     1141 ctgttgggag ctgtctgtgt gtatgtgtgt gtgtgtgagt atggagcagt aaaaagctgc
     1201 cgatacgaga agcgccgccc cggacggacg gcgatggcgc atcgccaaat gtgtcatttt
     1261 agttgtcttc acgaaccagg gcgcgcctct agaggatccc cgggattggc caaaggaccc
     1321 aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg acccttggct
     1381 agcgtcgacg gtaccggtag gtacaaaaaa gcaggcttaa tgtccaaagg agaagaactg
     1441 tttaccggtg ttgtgccaat tttggttgaa ctcgatggtg atgtcaacgg acataagttc
     1501 tcagtgagag gcgaaggaga aggtgacgcc accattggaa aattgactct taaattcatc
     1561 tgtactactg gtaaacttcc tgtaccatgg ccgactctcg taacaacgct tacgtacgga
     1621 gttcagtgct tttcgagata cccagaccat atgaaaagac atgacttttt taagtcggct
     1681 atgcctgaag gttacgtgca agaaagaaca atttcgttca aagatgatgg aaaatataaa
     1741 actagagcag ttgttaaatt tgaaggagat actttggtta accgcattga actgaaagga
     1801 acagatttta aagaagatgg taatattctt ggacacaaac tcgaatacaa ttttaatagt
     1861 cataacgtat acatcactgc tgataagcaa aagaacggaa ttaaagcgaa tttcacagta
     1921 cgccataatg tagaagatgg cagtgttcaa cttgccgacc attaccaaca aaacacccct
     1981 attggagacg gtccggtact tcttcctgat aatcactacc tctcaacaca aacagtcctg
     2041 agcaaagatc caaatgaaaa atacccagct ttcttgtaca aagtgggtga tatctgagct
     2101 ccgcatcggc cgctgtcatc agatcgccat ctcgcgcccg tgcctctgac ttctaagtcc
     2161 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     2221 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
     2281 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     2341 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     2401 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     2461 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     2521 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     2581 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     2641 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     2701 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     2761 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     2821 agaatacatt cccaatatac caaacataac tgtttccaac tttattatac atagttgata
     2881 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2941 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     3001 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     3061 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt gcaatctgct
     3121 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     3181 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3241 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     3301 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3361 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3421 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3481 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3541 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3601 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3661 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3721 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3781 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3841 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3901 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3961 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4021 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4081 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4141 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4201 gcggtatcat tgcagcactg gggtcagatg gtaagccctc ccgtatcgta gttatctaca
     4261 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4321 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4381 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4441 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4501 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4561 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4621 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4681 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4741 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4801 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4861 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4921 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4981 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5041 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5101 acgccagcaa cgcggccttt ttacggttcc tggtcttttg ctggcctttt gctcacatgt
     5161 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5221 ataccagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
     5281 caactttcca agcttgcatg ccgaggtcga cattcttgga atttcgcgtt ttccattttc
     5341 cgttgttttt tcattttata aaagtttcag cacctacatt tttcaatatt tcttcaatgg
     5401 agaataaatc agttgaatca gttgaaaaac aaagtcagag ctgtggaagt cgaaatttaa
     5461 gagctttttt tttaattttt tatagttgtt gtaaagctta ttagtatacc gcacctactg
     5521 ttaatatgaa cgatcaaatg ttactcttcc tttttcatta aaaaaaactt tcagtttgcc
     5581 ccgccggtgc gattccttat aagatgccac aagttttgtt atgcgacccg acaattgtta
     5641 atatctgtcc atacgactat aaggtttggt tttattcgca ttttattgtt ttgtttttgg
     5701 atcaactcta tcaaaaaaac aatattcgtt catgatacta atgactaatg agttttcagt
     5761 gcgttgaagc agcaaacgga caatatcttc ctgccgatgg ccgatctctt tgctgcaaga
     5821 ccaccactct gtactcgttt gcctcggttt ttggagaggc taaaataagc cctcggctag
     5881 tgccaaatcc ccctctttca gcgatcgaat atgtgcgtcg gtattttttg ttcctcttat
     5941 tttttgtctt agacatcaac gccgccgccg taaaacagaa gacatgtttc cgtacttcca
     6001 ggtcactttg aacgttcaca catccgccaa agagcactca ccggaaatta gaactggaga
     6061 tcattttgtg ctgacaccat acaaactcta cgagccggca tatttgaaaa aaatcaaact
     6121 gttcacagac tttggtcatg gctcatttgt gcatgttata ctttttggta aatctattga
     6181 acatgttcgg agtgttcatg cgagcgtaac taagttttga gaaaaaaaga atgagagaat
     6241 aactagggct tttgtctttt tttgtgaata tctggttatg tttgaagatc caatgtcaac
     6301 aactgaaaca atgcaacttt attatgaccg accaacatcc aatgggaaaa ctatcaattt
     6361 agatgagcca attccagacg gaggtttcaa taattaaaaa aaaattaaga ataatcaaat
     6421 aacatgcgct tttcaggatt catttctaag aaaatattca atgcggctcc aattacacaa
     6481 atagaaaacc catcacgtcc gggtccaata aaagaatgtg agaactttct tcttgaaaaa
     6541 tgaacatatt gaattgcacc aaacgcaaac aagaaactct aaatttcaga tcgaaaattg
     6601 tggattgtgc tcattttcaa gaccacaagc ccgtttggtc gagtttatat tctttccacc
     6661 aaggatttgc acagcaagta tagatcagtc accgattttt tgaaatccga cacaggaaac
     6721 atgctaggaa ctcctgtggc tggaacttat ttttatgtga gttttgtttt gaaccctcaa
     6781 ctataaaatt gtcggcccgt tttgttttca gctcaccacc gactgatttt catttcaaaa
     6841 ttaatgcaga tttctacgtg ttctttttca aataaattta tatatttttt gttctctcac
     6901 aaaataaata atttcgagag ttaagtgatc attctcagac atattctata attgtccgtc
     6961 aggaaacaga tagaggaacc tttgataaat aaattataaa aggccgacag ggaaatgctt
     7021 ctccctcgac tctgttagaa gcgaagaggc aatttttaaa aatttcccat ttttatgcgt
     7081 ttttctgatt tccaaagtca cgcatcattt accaaccgca acgattcata aggacgtaat
     7141 tttgattttc tggacgattt ttatatagtt ttgatgtgaa attcgcttta tttgtctgaa
     7201 agtgga
//
