LOCUS       sequence-248139-        4079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast plasmid overexpressing sLovA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang
            Y, Cai M
  TITLE     Engineered ethanol-driven biosynthetic system for improving 
            production of acetyl-CoA derived drugs in Crabtree-negative yeast.
  JOURNAL   Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. 
            Epub 2019 May 9.
  PUBMED    31077813
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-07"; volume: "54:275-284. doi"; pages: " 
            10.1016/j.ymben.2019.05.001. Epub 2019 May 9"
COMMENT     Sequence Label: pZ_lacO-cP-AOX1-sLovA
FEATURES             Location/Qualifiers
     source          1..4079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        392..439
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             458..832
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      898..1145
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(914..932)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(1220..1808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1300..1319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1904..1920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3693..3710
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3790..4036
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        join(4051..4079,1..385)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 tttctactcc ttttttactc ttccagattt tctcggactc cgcgcatcgc cgtaccactt
       61 caaaacaccc aagcacagca tactaaattt cccctctttc ttcctctagg gtgtcgttaa
      121 ttacccgtac taaaggtttg gaaaagaaaa aagagaccgc ctcgtttctt tttcttcgtc
      181 gaaaaaggca ataaaaattt ttatcacgtt tctttttctt gaaaattttt ttttttgatt
      241 tttttctctt tcgatgacct cccattgata tttaagttaa taaacggtct tcaatttctc
      301 aagtttcagt ttcatttttc ttgttctatt acaacttttt ttacttcttg ctcattagaa
      361 agaaagcata gcaatctaat ctaagggcgg tgttgacaat taatcatcgg catagtatat
      421 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
      481 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
      541 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
      601 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
      661 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
      721 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
      781 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtccg
      841 acgcggcccg acgggtccga ggcctcggag atccgtcccc cttttccttt gtcgatatca
      901 tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa
      961 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag
     1021 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg
     1081 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa
     1141 tttgcaagct ggagaccaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1201 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1261 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1321 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1381 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1441 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1501 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1561 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1621 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1681 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1741 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1801 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1861 cacgttaagg gattttggtc atgagatcag atcttgtgtg gaattgtgag cggataacaa
     1921 tttcacacac taacccctac ttgacagcaa tatataaaca gaaggaagct gccctgtctt
     1981 aaaccttttt ttttatcatc attattagct tactttcata attgcgactg gttccaattg
     2041 acaagctttt gattttaacg acttttaacg acaacttgag aagatcaaaa aacaactaat
     2101 tattcgaaat gactgttgac gctttgactc agccacacca cttgttgtcc ttggcctgga
     2161 acgatactca acagcacggt tcctggtttg cccctcttgt cactacctcc gccggtttgt
     2221 tgtgtttgtt gttgtacctt tgttcctccg gtagaagatc cgacttgcct gtcttcaatc
     2281 ctaagacctg gtgggagttg accactatga gagctaagcg tgatttcgat gctaacgccc
     2341 catcctggat tgagtcctgg ttctcccaga acgacaagcc aatcagattt attgttgact
     2401 ctggttactg caccatcttg ccttcctcta tggctgacga gttccgtaag atgaaggagt
     2461 tgtgcatgta caagttcttg ggtactgact tccactccca ccttccaggt tttgatggat
     2521 ttaaagaggt caccagagac gcccatttga tcaccaaggt tgtcatgaac cagttccaga
     2581 cccaggcccc aaagtatgtc aagccattgg ccaacgaggc ctccggaatt atcaccgaca
     2641 tcttcggtga ctctaacgag tggcataccg tcccagtcta caaccagtgc ttggacttgg
     2701 tcactcgtac cgtcaccttc attatggtcg gatctaagtt ggctcacaac gaagagtggt
     2761 tggacatcgc caagcatcac gccgttacca tggctatcca ggccagacag ttgcgtttgt
     2821 ggccagtcat tttgcgtcca ttggttcact ggcttgaacc tcaaggagcc aagttgagag
     2881 ctcaagttag aagagctaga caattgttgg atccaattat tcaagaacgt agagctgagc
     2941 gtgatgcttg cagagctaag ggtattgagc cacctagata cgtcgattcc attcagtggt
     3001 tcgaggacac tgccaaggga aaatggtacg acgctgctgg agctcaattg gctatggatt
     3061 tcgccggaat ctacggtacc tctgacttgt tgatcggtgg attggttgat atcgttcgtc
     3121 accctcactt gttggagcca ttgcgtgacg agatccgtac cgttattggt cagggtggtt
     3181 ggaccccagc ctctttgtac aagttgaagt tgcttgactc ctgtcttaag gagtctcagc
     3241 gtgttaagcc agtcgagtgt gccaccatga gatcttacgc tttgcaggac gtcaccttct
     3301 ccaacggtac cttcatccct aagggagaat tggtcgccgt tgccgccgac agaatgtcca
     3361 atcctgaggt ctggccagaa ccagccaagt acgaccctta ccgttacatg agattgcgtg
     3421 aggacccagc taaggccttc tctgcccagt tggagaacac caacggtgac catatcggtt
     3481 tcggttggca tcctagagct tgtcctggtc gtttcttcgc ctctaaggag atcaaaatga
     3541 tgcttgccta tttgttgatt agatacgact ggaaggtcgt cccagacgaa ccattgcagt
     3601 actacagaca ctccttttcc gtccgtatcc atcctaccac caagttgatg atgcgtcgtc
     3661 gtgacgagga cattagattg ccaggttctt tgcatcatca tcatcatcat tgagtttgta
     3721 gccttagaca tgactgttcc tcagttcaag ttgggcactt acgagaagac cggtcttgct
     3781 agattctaat caagaggatg tcagaatgcc atttgcctga gagatgcagg cttcattttt
     3841 gatacttttt tatttgtaac ctatatagta taggattttt tttgtcattt tgtttcttct
     3901 cgtacgagct tgctcctgat cagcctatct cgcagctgat gaatatcttg tggtaggggt
     3961 ttgggaaaat cattcgagtt tgatgttttt cttggtattt cccactcctc ttcagagtac
     4021 agaagattaa gtgagacctt cgtttgtgcg gatcccccac acaccatagc ttcaaaatg
//
