LOCUS       sequence-24814-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Keravala A, Portlock JL, Nash JA, Vitrant DG, Robbins PD, Calos MP
  TITLE     PhiC31 integrase mediates integration in cultured synovial cells and
            enhances gene expression in rabbit joints.
  JOURNAL   J Gene Med. 2006 Aug . 8(8):1008-17.
  PUBMED    16779871
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene Med.
            2006 Aug . 8(8):1008-17."
COMMENT     Sequence Label: pDB2
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    160..229
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(411..432)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     623..642
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcnaancncc cgcgtatccg cacccgccga cgccgtcgca cgtcccgtgc tcaccgtgac
       61 caccgcgccc agcggtttcg agggcgaggg cttcccggtg cgccgcgcgt tcgccgggat
      121 caactaccgc cacctcgacc cgttcatcat gatggaccag atgggtgagg tggagtacgc
      181 gcccggggag cccaagggca cgccctggca cccgcaccgc ggcttcgaga ccgtgaccta
      241 catcgtcgac ggtacctgaa gccgaattcg cgtaaattgt aagcgttaat attttgttaa
      301 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
      361 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
      421 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
      481 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
      541 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
      601 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
      661 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgcnngnan
      721 ggggncnnnt cnggnnntnt gttngganaa tgtgntggga ancccctatt ngtttntttt
      781 ncnaatacat ncaaattgta nccgctcatg aaaanaaccc tgataatgct ncata
//
