LOCUS       sequence-248147-        9794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the recombinant APOBEC3A in vitro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C
  TITLE     Efficient C-to-T base editing in plants using a fusion of nCas9 and 
            human APOBEC3A.
  JOURNAL   Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261.
  PUBMED    30272679
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261."
COMMENT     Sequence Label: pET42b-A3A-PBE-Delta-UGI
FEATURES             Location/Qualifiers
     source          1..9794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(766..789,790..792,793..4866)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4867..4914
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4962..4980)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4976..5023
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5060..5515
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5147..5166)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5357..5378
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5607..6422)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5607..6422)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6330..6349
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6414..6433
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6544..7132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7033..7052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7286..7303
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7318..7457
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7383..7402)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7543..7565
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7559..7750)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8322..9404)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9366..9385
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9405..9482)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9688..9707
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacaatg ggcagcagtc atcatcatca ccatcacgag
      121 gccagcccgg ctagcggccc aaggcatctc atggacccgc acatcttcac cagcaacttc
      181 aacaacggca tcggcaggca caagacctac ttgtgctacg aggtggagag gctcgacaac
      241 ggaacctccg tgaagatgga ccaacacagg gggttcctcc acaaccaagc caagaacctc
      301 ctctgcggct tctacggcag gcacgccgag ttgaggttcc tcgacttggt gccatccctc
      361 caactcgatc cagcccaaat ctaccgcgtg acctggttca tctcctggtc cccatgcttc
      421 tcctggggtt gcgccggcga ggttcgggct ttcctccaag aaaacaccca cgtccgcctc
      481 cgcattttcg ccgccaggat ctatgattac gaccctctct acaaggaggc cctccagatg
      541 ctgcgggacg ccggtgctca ggtgagtatc atgacctacg acgagttcaa gcactgctgg
      601 gacaccttcg ttgaccacca gggctgccca ttccaaccat gggacggtct ggatgaacac
      661 agccaagcct tgtccggcag gctccgggcc atcctccaaa accaggggaa ctccgggagc
      721 gagacgccag gcacctccga gtcggccacc ccagaatctc ttaaggacaa gaagtactcg
      781 atcggcctcg ccatcgggac gaactcagtt ggctgggccg tgatcaccga cgagtacaag
      841 gtgccctcta agaagttcaa ggtcctgggg aacaccgacc gccattccat caagaagaac
      901 ctcatcggcg ctctcctgtt cgacagcggg gagaccgctg aggctacgag gctcaagaga
      961 accgctaggc gccggtacac gagaaggaag aacaggatct gctacctcca agagattttc
     1021 tccaacgaga tggccaaggt tgacgattca ttcttccacc gcctggagga gtctttcctc
     1081 gtggaggagg ataagaagca cgagcggcat cccatcttcg gcaacatcgt ggacgaggtt
     1141 gcctaccacg agaagtaccc tacgatctac catctgcgga agaagctcgt ggactccacc
     1201 gataaggcgg acctcagact gatctacctc gctctggccc acatgatcaa gttccgcggc
     1261 catttcctga tcgaggggga tctcaaccca gacaacagcg atgttgacaa gctgttcatc
     1321 caactcgtgc agacctacaa ccaactcttc gaggagaacc cgatcaacgc ctctggcgtg
     1381 gacgcgaagg ctatcctgtc cgcgaggctc tcgaagtcca ggaggctgga gaacctgatc
     1441 gctcagctcc caggcgagaa gaagaacggc ctgttcggga acctcatcgc tctcagcctg
     1501 gggctcaccc cgaacttcaa gtcgaacttc gatctcgctg aggacgccaa gctgcaactc
     1561 tccaaggaca cctacgacga tgacctcgat aacctcctgg cccagatcgg cgatcaatac
     1621 gcggacctgt tcctcgctgc caagaacctg tcggacgcca tcctcctgtc agatatcctc
     1681 cgcgtgaaca ccgagatcac gaaggctcca ctctctgcct ccatgatcaa gcgctacgac
     1741 gagcaccatc aggatctgac cctcctgaag gcgctggtcc gccaacagct cccggagaag
     1801 tacaaggaga ttttcttcga tcagtcgaag aacggctacg ctgggtacat cgacggcggg
     1861 gcctcacaag aggagttcta caagttcatc aagccaatcc tggagaagat ggacggcacg
     1921 gaggagctcc tggtgaagct caacagggag gacctcctgc ggaagcagag aaccttcgat
     1981 aacggcagca tcccccacca aatccatctc ggggagctgc acgccatcct gagaaggcaa
     2041 gaggacttct accctttcct caaggataac cgggagaaga tcgagaagat cctgaccttc
     2101 agaatcccat actacgtcgg ccctctcgcg cgggggaact caagattcgc ttggatgacc
     2161 cgcaagtctg aggagaccat cacgccgtgg aacttcgagg aggtggtgga caagggcgct
     2221 agcgctcagt cgttcatcga gaggatgacc aacttcgaca agaacctgcc caacgagaag
     2281 gtgctcccta agcactcgct cctgtacgag tacttcaccg tctacaacga gctcacgaag
     2341 gtgaagtacg tcaccgaggg catgcgcaag ccagcgttcc tgtccgggga gcagaagaag
     2401 gctatcgtgg acctcctgtt caagaccaac cggaaggtca cggttaagca actcaaggag
     2461 gactacttca agaagatcga gtgcttcgat tcggtcgaga tcagcggcgt tgaggaccgc
     2521 ttcaacgcca gcctcgggac ctaccacgat ctcctgaaga tcatcaagga taaggacttc
     2581 ctggacaacg aggagaacga ggatatcctg gaggacatcg tgctgaccct cacgctgttc
     2641 gaggacaggg agatgatcga ggagcgcctg aagacgtacg cccatctctt cgatgacaag
     2701 gtcatgaagc aactcaagcg ccggagatac accggctggg ggaggctgtc ccgcaagctc
     2761 atcaacggca tccgggacaa gcagtccggg aagaccatcc tcgacttcct caagagcgat
     2821 ggcttcgcca acaggaactt catgcaactg atccacgatg acagcctcac cttcaaggag
     2881 gatatccaaa aggctcaagt gagcggccag ggggactcgc tgcacgagca tatcgcgaac
     2941 ctcgctggct cccccgcgat caagaagggc atcctccaga ccgtgaaggt tgtggacgag
     3001 ctcgtgaagg tcatgggccg gcacaagcct gagaacatcg tcatcgagat ggccagagag
     3061 aaccaaacca cgcagaaggg gcaaaagaac tctagggagc gcatgaagcg catcgaggag
     3121 ggcatcaagg agctggggtc ccaaatcctc aaggagcacc cagtggagaa cacccaactg
     3181 cagaacgaga agctctacct gtactacctc cagaacggca gggatatgta cgtggaccaa
     3241 gagctggata tcaaccgcct cagcgattac gacgtcgatc atatcgttcc ccagtctttc
     3301 ctgaaggatg actccatcga caacaaggtc ctcaccaggt cggacaagaa ccgcggcaag
     3361 tcagataacg ttccatctga ggaggtcgtt aagaagatga agaactactg gaggcagctc
     3421 ctgaacgcca agctgatcac gcaaaggaag ttcgacaacc tcaccaaggc tgagagaggc
     3481 gggctctcag agctggacaa ggccggcttc atcaagcggc agctggtcga gaccagacaa
     3541 atcacgaagc acgttgcgca aatcctcgac tctcggatga acacgaagta cgatgagaac
     3601 gacaagctga tcagggaggt taaggtgatc accctgaagt ctaagctcgt ctccgacttc
     3661 aggaaggatt tccagttcta caaggttcgc gagatcaaca actaccacca tgcccatgac
     3721 gcttacctca acgctgtggt cggcaccgct ctgatcaaga agtacccaaa gctggagtcc
     3781 gagttcgtgt acggggacta caaggtttac gatgtgcgca agatgatcgc caagtcggag
     3841 caagagatcg gcaaggctac cgccaagtac ttcttctact caaacatcat gaacttcttc
     3901 aagaccgaga tcacgctggc caacggcgag atccggaaga gaccgctcat cgagaccaac
     3961 ggcgagacgg gggagatcgt gtgggacaag ggcagggatt tcgcgaccgt ccgcaaggtt
     4021 ctctccatgc cccaggtgaa catcgtcaag aagaccgagg tccaaacggg cgggttctca
     4081 aaggagtcta tcctgcctaa gcggaacagc gacaagctca tcgccagaaa gaaggactgg
     4141 gacccaaaga agtacggcgg gttcgacagc cctaccgtgg cctactcggt cctggttgtg
     4201 gcgaaggttg agaagggcaa gtccaagaag ctcaagagcg tgaaggagct cctggggatc
     4261 accatcatgg agaggtccag cttcgagaag aacccaatcg acttcctgga ggccaagggc
     4321 tacaaggagg tgaagaagga cctgatcatc aagctcccga agtactctct cttcgagctg
     4381 gagaacggca ggaagagaat gctggcttcc gctggcgagc tccagaaggg gaacgagctc
     4441 gcgctgccaa gcaagtacgt gaacttcctc tacctggctt cccactacga gaagctcaag
     4501 ggcagcccgg aggacaacga gcaaaagcag ctgttcgtcg agcagcacaa gcattacctc
     4561 gacgagatca tcgagcaaat ctccgagttc agcaagcgcg tgatcctcgc cgacgcgaac
     4621 ctggataagg tcctctccgc ctacaacaag caccgggaca agcccatcag agagcaagcg
     4681 gagaacatca tccatctctt caccctgacg aacctcggcg ctcctgctgc tttcaagtac
     4741 ttcgacacca cgatcgatcg gaagagatac acctccacga aggaggtcct ggacgcgacc
     4801 ctcatccacc agtcgatcac cggcctgtac gagacgagga tcgacctctc acaactcggc
     4861 ggggataaga gacccgcagc aaccaagaag gcagggcaag caaagaagaa gaagtaggct
     4921 gctaaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     4981 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     5041 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5101 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5161 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5221 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5281 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5341 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5401 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5461 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     5521 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5581 atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa atgaaactgc
     5641 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     5701 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     5761 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     5821 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt
     5881 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     5941 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta
     6001 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     6061 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     6121 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     6181 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     6241 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     6301 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     6361 gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc
     6421 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgaccaaaat
     6481 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6541 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6601 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6661 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     6721 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6781 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6841 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6901 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6961 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7021 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7081 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7141 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7201 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7261 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     7321 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcaatg gtgcactctc
     7381 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     7441 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7501 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7561 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     7621 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     7681 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     7741 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     7801 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     7861 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
     7921 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
     7981 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     8041 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     8101 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     8161 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcta gtcatgcccc
     8221 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     8281 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     8341 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8401 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     8461 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     8521 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     8581 tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc ggtaatggcg
     8641 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     8701 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     8761 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     8821 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     8881 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     8941 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     9001 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     9061 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     9121 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     9181 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     9241 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     9301 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     9361 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     9421 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     9481 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     9541 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
     9601 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     9661 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     9721 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatcgatct
     9781 cgatcccgcg aaat
//
