LOCUS       sequence-248150-        4915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pttr-39::gfp1-10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   He S, Cuentas-Condori A, Miller DM
  TITLE     NATF (Native And Tissue-Specific Fluorescence): A Strategy for 
            Bright, Tissue-Specific GFP Labeling of Native Proteins in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Apr 5. pii: genetics.119.302063. doi: 
            10.1534/genetics.119.302063.
  PUBMED    30952669
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Apr 5. pii: genetics.119.302063. doi: 
            10.1534/genetics.119.302063."
COMMENT     Sequence Label: pSH79
FEATURES             Location/Qualifiers
     source          1..4915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     71..93
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     90..106
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     90..106
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1180..1821
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     protein_bind    complement(1823..1847)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2617..2637)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2645..2662)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2645..2661)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2654..2676)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2989..3011
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3049..3067)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3135..3239
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3240..3308,3309..4100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3458..3477)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4271..4859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4760..4779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      121 gctatcaact ttccaagctt gcatgcatta ttatttctat cggctacttt tttcttttta
      181 gctgccttct gctacgcgga gaattggaaa ttttcgaaac tgattttgaa tatcgattct
      241 gattgtattt ctgataaaga cgtagaaagt tcgaagggtc ggattacggg gtatttcaaa
      301 ggttacacga cttaaattta aatagtttta tctattctcg ttggcacgaa gtatatctag
      361 gcaatcgtga cgaaggaaac ctttgacgtt tgtgtagaga aaagcaaact attctggaac
      421 acttcaaaag atctctaaaa tacaaatatc gaatttgtat ttttgtatga aaaagtggat
      481 taaaagggtg ttcgcttcga gaaagataca aaatgctgaa attagtatat agaagttcct
      541 aaagtttgaa agcagagcca agcgttactg aatttggaaa aaggaaattt cctgcatagg
      601 tgaagcctaa taggtgaaat ggatgtaaca tgaaaatgtt ctaaaacatt ttcaccgtaa
      661 aatttaggta ggcgggaagg tcctgttgtg cccatatagg cttatgatct ctctgaaaaa
      721 ctctctgaac tttgaatttc tccagaatgt ttttggtttc tgatcgataa atgtttactg
      781 gttgttgatg tttgtaaaag tcctgcaaat tctagaaaat tctcaaactc tttttcttat
      841 tttcatgaga aaaccgaaac tttgaagtac ataagcaaag agttgcgttg ttattttaat
      901 cttttgaacc tattaataaa tcttttttgt tttctcggga ttatgttagg attacaaaga
      961 catttaagac aagaagttgt ttctgttatt tcactttttt tgtttgcgca ttcacaattt
     1021 tgttaaaaca aaaaatcatg gcgcgcctct agaggatccc cgggattggc caaaggaccc
     1081 aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg acccttggct
     1141 agcgtcgacg gtaccggtag gtacaaaaaa gcaggcttaa tgtccaaagg agaagaactg
     1201 tttaccggtg ttgtgccaat tttggttgaa ctcgatggtg atgtcaacgg acataagttc
     1261 tcagtgagag gcgaaggaga aggtgacgcc accattggaa aattgactct taaattcatc
     1321 tgtactactg gtaaacttcc tgtaccatgg ccgactctcg taacaacgct tacgtacgga
     1381 gttcagtgct tttcgagata cccagaccat atgaaaagac atgacttttt taagtcggct
     1441 atgcctgaag gttacgtgca agaaagaaca atttcgttca aagatgatgg aaaatataaa
     1501 actagagcag ttgttaaatt tgaaggagat actttggtta accgcattga actgaaagga
     1561 acagatttta aagaagatgg taatattctt ggacacaaac tcgaatacaa ttttaatagt
     1621 cataacgtat acatcactgc tgataagcaa aagaacggaa ttaaagcgaa tttcacagta
     1681 cgccataatg tagaagatgg cagtgttcaa cttgccgacc attaccaaca aaacacccct
     1741 attggagacg gtccggtact tcttcctgat aatcactacc tctcaacaca aacagtcctg
     1801 agcaaagatc caaatgaaaa atacccagct ttcttgtaca aagtgggtga tatctgagct
     1861 ccgcatcggc cgctgtcatc agatcgccat ctcgcgcccg tgcctctgac ttctaagtcc
     1921 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     1981 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
     2041 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     2101 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     2161 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     2221 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     2281 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     2341 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     2401 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     2461 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     2521 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     2581 agaatacatt cccaatatac caaacataac tgtttccaac tttattatac atagttgata
     2641 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2701 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2761 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     2821 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt gcaatctgct
     2881 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     2941 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3001 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     3061 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3121 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3181 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3241 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3301 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3361 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3421 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3481 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3541 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3601 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3661 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3721 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3781 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3841 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3901 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3961 gcggtatcat tgcagcactg gggtcagatg gtaagccctc ccgtatcgta gttatctaca
     4021 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4081 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4141 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4201 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4261 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4321 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4381 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4441 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4501 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4561 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4621 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4681 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4741 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4801 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4861 acgccagcaa cgcggccttt ttacggttcc tggtcttttg ctggcctttt gctca
//
