LOCUS       sequence-248151-        6318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pflp-13::gfp1-10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   He S, Cuentas-Condori A, Miller DM
  TITLE     NATF (Native And Tissue-Specific Fluorescence): A Strategy for 
            Bright, Tissue-Specific GFP Labeling of Native Proteins in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Apr 5. pii: genetics.119.302063. doi: 
            10.1534/genetics.119.302063.
  PUBMED    30952669
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Apr 5. pii: genetics.119.302063. doi: 
            10.1534/genetics.119.302063."
COMMENT     Sequence Label: pSH87
FEATURES             Location/Qualifiers
     source          1..6318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2118..2759
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     protein_bind    complement(2761..2785)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3555..3575)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3583..3600)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3583..3599)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3592..3614)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3927..3949
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3987..4005)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4073..4177
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4178..4246,4247..5038)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4396..4415)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5209..5797
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5698..5717
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5924..5946
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5943..5959
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5943..5959
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcattatttc acttgttgca gtcaaaatgt tcagacttgc aaccgctttt ttctttaacc
       61 ccatttaaat aggtcatttt gaaactgaaa ttttagatat caatgtaaca attttcagaa
      121 aatcatgaaa atgagaaaat aacactgaat ctcaattttt tctaactagc cgtttattaa
      181 ttaaaactgt agtagcattt tagtcggcat tttgtgtcaa aacaaattca ttcttgtatt
      241 tttttttaat gcagtcaata tcttagtaaa ttgccaaata tacgaaacct atggagcgtt
      301 tctgaggaat tcagaacact agaacactgt tcaatccttg ctacatttaa atacagggtg
      361 ggccaaaagt atggtaacac gtttgcagct ctataacttt actagtggag agagttatta
      421 catcaatttg tacaaggttt gttctccata aaatactgac cagatgaacg ttttgaattt
      481 tcaaaatatt cccatccgca tcgctgcagg ccttcagaac ttttgagatc acgaccttta
      541 tggcacgcaa gtcggcattc tgcgggattt ctacattttt ctcaaggaat ccatcaagtg
      601 tcttacaagt ttttatagtt caaagaatgc tcttggtcta gagatttttt aaaaacaaaa
      661 atccaaataa tttctatcac ggtatttctg tcatggactg cggcagtagg ttaagtcttg
      721 aatggcatag tcgccagaaa gatggatgtg agcggcgagt agcagtatgc agaaaaattc
      781 attttagtat ttttcgagac gttttgacct gactttccag aattttttta gaaaattgag
      841 ctcgaatgat tttgtgggtc gttacgcctt gcgaaaccaa aaaaactgag taaatttcgc
      901 aaagttgaat tcatgaaaaa ttcaaaggtt ctggcgccag tgtcgacaca gaaaattttt
      961 gtatgctaca aaaacaatca aaaacgcttc ttcacttctt taactttaaa tttaacataa
     1021 aaatcataca tctgaaaatt attggcgtgc gtgggaggtg tgtttgtgtg ggaagtgccg
     1081 cgtgccaaat taccgttttt tcgaaagggt tcgccaaatt ttcgaggtga taaagatcgc
     1141 accttattgg gaaacattac aaattttgag tcaaattgaa ggaaatttat tcagctatca
     1201 gtgtttgata gaagaatgtg cgtatgacgt ggcagtcgcg cgaaaagctc atgtaaaaga
     1261 acgcttcatg tgttaccata cttttggccc accctgtata aatcgtgaaa acataattag
     1321 attataaaag taaaagtcgc ccggtttcga ttatggttgg caaaatctga gtaatcgcta
     1381 cttttttttg attgcaatta aaaataaatt ctgacacctt ttaaaaacat gtaccatgat
     1441 attttttaaa cgatttcgcg acttggacta catcttttga actccaatat tatcagttcc
     1501 gaagtgtgat gttgatgtta acataatgac atttacaacg tattctgatt tctttgaata
     1561 tcttctcgaa ctctcgagac acatctgttc agattccagt tgacttgtca catttacgca
     1621 tttccctcct ccattctttt attttgtttc tatttcttgc gtaaatctga ttaaaatcga
     1681 gaagtaggcg cgcattttct gtcttcacct tttgactata aaagtactct acaaatattg
     1741 aacaacactt tttaaaagtt gtagtagtta ttggccatca ggaattagga ggtcagttgc
     1801 aaaatcatgc atcttttagg attaaaagct taaatcttgc tcattcattt cctgtcttat
     1861 tttgaagttt ttctctcttc caacgcctcc cactgtgact actatcatct attattatct
     1921 tgtagggatt cctcaatttc cagacaccct caaactcggc cccgttccga ttcgaacggc
     1981 gcgcctctag aggatccccg ggattggcca aaggacccaa aggtatgttt cgaatgatac
     2041 taacataaca tagaacattt tcaggaggac ccttggctag cgtcgacggt accggtaggt
     2101 acaaaaaagc aggcttaatg tccaaaggag aagaactgtt taccggtgtt gtgccaattt
     2161 tggttgaact cgatggtgat gtcaacggac ataagttctc agtgagaggc gaaggagaag
     2221 gtgacgccac cattggaaaa ttgactctta aattcatctg tactactggt aaacttcctg
     2281 taccatggcc gactctcgta acaacgctta cgtacggagt tcagtgcttt tcgagatacc
     2341 cagaccatat gaaaagacat gactttttta agtcggctat gcctgaaggt tacgtgcaag
     2401 aaagaacaat ttcgttcaaa gatgatggaa aatataaaac tagagcagtt gttaaatttg
     2461 aaggagatac tttggttaac cgcattgaac tgaaaggaac agattttaaa gaagatggta
     2521 atattcttgg acacaaactc gaatacaatt ttaatagtca taacgtatac atcactgctg
     2581 ataagcaaaa gaacggaatt aaagcgaatt tcacagtacg ccataatgta gaagatggca
     2641 gtgttcaact tgccgaccat taccaacaaa acacccctat tggagacggt ccggtacttc
     2701 ttcctgataa tcactacctc tcaacacaaa cagtcctgag caaagatcca aatgaaaaat
     2761 acccagcttt cttgtacaaa gtgggtgata tctgagctcc gcatcggccg ctgtcatcag
     2821 atcgccatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac
     2881 atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta
     2941 atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa
     3001 aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata
     3061 ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct
     3121 gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta
     3181 cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca
     3241 aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag
     3301 tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat
     3361 ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag
     3421 atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa
     3481 agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca
     3541 aacataactg tttccaactt tattatacat agttgataat tcactggccg tcgttttaca
     3601 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3661 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3721 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3781 ttcacaccgc atatggtgca ctctcagtgc aatctgctct gatgccgcat agttaagcca
     3841 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3901 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3961 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     4021 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     4081 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4141 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4201 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4261 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4321 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4381 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4441 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4501 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4561 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4621 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4681 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4741 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4801 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4861 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4921 gtcagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4981 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5041 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5101 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5161 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5221 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5281 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5341 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5401 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5461 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5521 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5581 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5641 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5701 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5761 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5821 acggttcctg gtcttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5881 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accagcggat aacaatttca
     5941 cacaggaaac agctatgacc atgattacgc caagctatca actttccaag cttgcatgca
     6001 aattgtgcct cctgatgctg aatcctcaat ccatttctcc atacttcctt ttcacttcct
     6061 ccatttggcg gaaggtgggc ggtcctcccg ccgaaacttg atctcttttt tatttttaag
     6121 aagatccttt atgtttttta tattgacgtt tacttttttt ttggaagaaa gtgggtgaac
     6181 tacaatcaga ttatttttat gctccgcgaa aatttaatca tgatcaaatg tgatatatga
     6241 tgataatttg ggaataaaaa agtggaggtg gaacataatt tgtgttctta cggactataa
     6301 tttatttgat ataaaaca
//
