LOCUS       sequence-248166-        8896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ATP measurement, nucleus, lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Yaginuma H,  Okada Y
  TITLE     Live cell imaging of metabolic heterogeneity by quantitative 
            fluorescent ATP indicator protein, QUEEN-37C
  JOURNAL   bioRxiv 2021.10.08.463131
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.10.08.463131"
COMMENT     Sequence Label: NLS-QUE37C/pLBS CAG
FEATURES             Location/Qualifiers
     source          1..8896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     LTR             892..1072
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1116..1241
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1734..1967
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2151..2195
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2456..2572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2651..2954
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2957..3232
                     /label=chicken beta-actin promoter
     intron          3233..4241
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4165..4187
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4297..4313
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4298..4314
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4344..4364
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4377..4386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4743..4994
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     4931..4952
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5058..5079)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5319..5338)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5565..6153
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5618..5638)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6036..6047)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6244..6477
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6549..6630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6586..6605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6640..6659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6708..6728)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6708..6726)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6747..6763)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6747..6763)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6760..6782)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6771..6787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6795..6801,6802..6819,6820..6825))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6840..6861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6978..6995)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7149..7737)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7229..7248)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7908..8699,8700..8768))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8531..8550
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8769..8873)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccac
      661 gcgtgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
      721 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
      781 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
      841 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacaataaa cgggtctctc
      901 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      961 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     1021 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1081 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1141 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1201 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1261 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1321 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1381 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1441 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1501 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1561 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1621 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1681 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1741 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1801 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1861 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1921 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1981 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     2041 tctctggaac agattggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     2101 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     2161 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2221 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2281 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2341 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2401 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg ttaactttta
     2461 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2521 cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttgggccctt
     2581 ccaaccttcc aaccggtacc tagtaatcaa ttacggggtc attagttcat agcccatata
     2641 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2701 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2761 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
     2821 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2881 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2941 tcgctattac catgggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     3001 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     3061 gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
     3121 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
     3181 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
     3241 tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc cccggctctg
     3301 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     3361 ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa gccttaaagg
     3421 gctccgggag ggccctttgt gcggggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
     3481 gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
     3541 cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg cggccggggg cggtgccccg
     3601 cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     3661 gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga
     3721 gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc
     3781 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     3841 gccggggagg gctcggggga ggggcgcggc ggccccggag cgccggcggc tgtcgaggcg
     3901 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     3961 tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     4021 cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     4081 ccgcgccgcc gtccccttct ccatctccag cctcggggct gccgcagggg gacggctgcc
     4141 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     4201 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     4261 ttgttgtgct gtctcatcat tttggcaaag gattcctcga ggtcgacggt atcgataagc
     4321 ttgatatcga attcaccatg gcgccgaaaa aaaaacgcaa agtggatcca ccggtcgcca
     4381 ccatggtgaa aactgtgaaa gtgaatataa caacccctga tgggccagtc tacgacgctg
     4441 atatcgagat ggtgtccgtg cgggccgaga gtggtgatct cggcatcctc cccggtcaca
     4501 ttcccacaaa ggccccactg aagatcggag ctgtgcggct gaagaaggac ggccaaacag
     4561 aggatgtcgc agtctcaggc ggcactgttg aagtgcggcc tgaccacgtt accattaatg
     4621 ctcaagccgc tgaacgggcg gaggacattg acgtcctccg cgccaaagcg gcgaaagagc
     4681 gggcggagcg ccgcctgcaa agccagaccc gttacaacag ccacaacgtc tatatcatgg
     4741 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     4801 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4861 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga
     4921 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4981 acgagctgta caagggtggc accggaggga gcatggtgag caagggcgag gagctgttca
     5041 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     5101 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     5161 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     5221 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     5281 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     5341 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     5401 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacctg ggtgacatcg
     5461 acttcaaacg ggccgaactg gcgttaaaac gcgccatgaa ccgtttgagc gttgcggaag
     5521 ataagtgagc ggccgcgact ctagatcact agtctcgact cgacaatcaa cctctggatt
     5581 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5641 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5701 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5761 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5821 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5881 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5941 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     6001 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6061 cttcccgcgg cctgctgccg gctctgcggc ctcttccgca tcttcgcctt cgccctcaga
     6121 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattcgagc tcggtacctt
     6181 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6241 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt
     6301 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6361 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6421 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6481 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6541 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6601 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6661 atgtatctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     6721 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6781 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6841 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6901 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6961 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7021 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7081 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7141 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7201 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7261 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7321 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7381 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7441 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7501 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7561 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     7621 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7681 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7741 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7801 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7861 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7921 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7981 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8041 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8101 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8161 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8221 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8281 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8341 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8401 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8461 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8521 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8581 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8641 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8701 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8761 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8821 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8881 ccccgaaaag tgccac
//
