LOCUS       sequence-248172-        3933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part P- Promoter (non-constitutive); Ptac; IPTG/lactose 
            induced; controlled by LacI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-Ptac_P
FEATURES             Location/Qualifiers
     source          1..3933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        join(88..93,94..109,110..116)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    122..141
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_feature    166..186
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             214..234
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             254..961
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             254..961
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(278..306)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     901..921
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1001..1095
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1168..1259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1260..1328,1329..2120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2124..2163
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2310..2418
                     /note="incP origin of transfer"
     rep_origin      2454..3042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2943..2962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3196..3213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3228..3368
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3294..3313)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3454..3476
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3470..3661)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3841..3927)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagtgttg acaattaatc atcggctcgt ataatgtgtg
      121 gaattgtgag cgctcacaat ttacttgaga cctttcatcg cgacctacta gtagcggccg
      181 ctgcagggag taacttctga ctgagttgca tacttgttta actttaagaa ggagatatac
      241 aatggtagcc cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      301 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      361 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      421 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
      481 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      541 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      601 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      661 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
      721 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
      781 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      841 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagatcacat
      901 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
      961 aggttcgtaa cttctgactg agttgcacgc tctagtcttg gactcctgtt gatagatcca
     1021 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
     1081 tttattggtg agaatccagg ggtccccaat aattacgatt taaattagta gcccgcctaa
     1141 tgagcgggct tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt
     1201 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1261 tgagcattca gcattttcgt gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg
     1321 tgtttgcgca tccggaaacc ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc
     1381 gcgtgggcta tattgaactg gatctgaaca gcggcaaaat tctggaatct tttcgtccgg
     1441 aagaacgttt tccgatgatg agcaccttta aagtgctgct gtgcggtgcg gttctgagcc
     1501 gtgtggatgc gggccaggaa caactgggcc gtcgtattca ttatagccag aacgatctgg
     1561 tggaatatag cccggtgacc gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt
     1621 gcagcgcggc gattaccatg agcgataaca ccgcggcgaa cctgctgctg acgaccattg
     1681 gcggtccgaa agaactgacc gcgtttctgc ataacatggg cgatcatgtg acccgtctgg
     1741 atcgttggga accggaactg aacgaagcga ttccgaacga tgaacgtgat accaccatgc
     1801 cggcagcaat ggcgaccacc ctgcgtaaac tgctgacggg tgagctgctg accctggcaa
     1861 gccgccagca actgattgat tggatggaag cggataaagt ggcgggtccg ctgctgcgta
     1921 gcgcgctgcc ggctggctgg tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc
     1981 gtggcattat tgcggcgctg ggcccggatg gtaaaccgag ccgtattgtg gtgatttata
     2041 ccaccggcag ccaggcgacg atggatgaac gtaaccgtca gattgcggaa attggcgcga
     2101 gcctgattaa acattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt
     2161 ggacgaccct tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt
     2221 tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac
     2281 tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg
     2341 agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct
     2401 gctctgcgag gctggccgta ggccggcccc gtagaaaaga tcaaaggatc ttcttgagat
     2461 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2521 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2581 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2641 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2701 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2761 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2821 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2881 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2941 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3001 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3061 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3121 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3181 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     3241 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     3301 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     3361 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     3421 cggcatccgc ttacagacaa gctgtgacag tctccgggag ctgcatgtgt cagaggtttt
     3481 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgca
     3541 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     3601 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     3661 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     3721 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     3781 agggtaaaca actggcggta tggatgcggc gggggcgcgc ccagctgtct agggcggcgg
     3841 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     3901 tttcgactga gcctttcgtt ttatttgatg cct
//
