LOCUS       sequence-248177-        3926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part P- Promoter (non-constitutive); Pci; controlled by cI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-Pci_P
FEATURES             Location/Qualifiers
     source          1..3926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    159..179
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             207..227
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             247..954
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             247..954
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(271..299)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     894..914
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      994..1088
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1161..1252
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1253..1321,1322..2113)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2117..2156
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2303..2411
                     /note="incP origin of transfer"
     rep_origin      2447..3035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2936..2955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3189..3206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3221..3361
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3287..3306)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3447..3469
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3463..3654)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3834..3920)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagtaaca ccgtgcgtgt tgactatttt acctctggcg
      121 gtgataatgg ttgctacttg agacctttca tcgcgaccta ctagtagcgg ccgctgcagg
      181 gagtaacttc tgactgagtt gcatacttgt ttaactttaa gaaggagata tacaatggta
      241 gcccgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      301 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      361 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      421 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      481 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      541 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      601 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      661 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      721 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      781 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      841 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagatca catggtcctt
      901 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaaggttcg
      961 taacttctga ctgagttgca cgctctagtc ttggactcct gttgatagat ccagtaatga
     1021 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
     1081 gtgagaatcc aggggtcccc aataattacg atttaaatta gtagcccgcc taatgagcgg
     1141 gctttttttt aattccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1201 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagcat
     1261 tcagcatttt cgtgtggcgc tgattccgtt ttttgcggcg ttttgcctgc cggtgtttgc
     1321 gcatccggaa accctggtga aagtgaaaga tgcggaagat caactgggtg cgcgcgtggg
     1381 ctatattgaa ctggatctga acagcggcaa aattctggaa tcttttcgtc cggaagaacg
     1441 ttttccgatg atgagcacct ttaaagtgct gctgtgcggt gcggttctga gccgtgtgga
     1501 tgcgggccag gaacaactgg gccgtcgtat tcattatagc cagaacgatc tggtggaata
     1561 tagcccggtg accgaaaaac atctgaccga tggcatgacc gtgcgtgaac tgtgcagcgc
     1621 ggcgattacc atgagcgata acaccgcggc gaacctgctg ctgacgacca ttggcggtcc
     1681 gaaagaactg accgcgtttc tgcataacat gggcgatcat gtgacccgtc tggatcgttg
     1741 ggaaccggaa ctgaacgaag cgattccgaa cgatgaacgt gataccacca tgccggcagc
     1801 aatggcgacc accctgcgta aactgctgac gggtgagctg ctgaccctgg caagccgcca
     1861 gcaactgatt gattggatgg aagcggataa agtggcgggt ccgctgctgc gtagcgcgct
     1921 gccggctggc tggtttattg cggataaaag cggtgcgggc gaacgtggca gccgtggcat
     1981 tattgcggcg ctgggcccgg atggtaaacc gagccgtatt gtggtgattt ataccaccgg
     2041 cagccaggcg acgatggatg aacgtaaccg tcagattgcg gaaattggcg cgagcctgat
     2101 taaacattgg taaaccgata caattaaagg ctccttttgg agcctttttt tttggacgac
     2161 ccttgtcctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
     2221 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
     2281 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
     2341 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
     2401 gaggctggcc gtaggccggc cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2581 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2881 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2941 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3001 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3061 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3121 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3181 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     3241 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     3301 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     3361 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3421 cgcttacaga caagctgtga cagtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3481 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc
     3541 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     3601 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     3661 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     3721 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     3781 acaactggcg gtatggatgc ggcgggggcg cgcccagctg tctagggcgg cggatttgtc
     3841 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     3901 tgagcctttc gttttatttg atgcct
//
