LOCUS       sequence-24825-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Dundr M, Ospina JK, Sung MH, John S, Upender M, Ried T, Hager GL, 
            Matera AG
  TITLE     Actin-dependent intranuclear repositioning of an active gene locus 
            in vivo.
  JOURNAL   J Cell Biol. 2007 Dec 17. 179(6):1095-103.
  PUBMED    18070915
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2007 Dec 17. 179(6):1095-103."
COMMENT     Sequence Label: Cherry-LacRep
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..59
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     polyA_signal    189..310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(226..245)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     280..299
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(317..772)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(454..475)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     666..685
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acgcgggcgc tggtggcctg aatgacatct ttgaggccca gaagatcgag tggcatgagg
       61 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
      121 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      181 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      241 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      301 atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
      361 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
      421 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
      481 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
      541 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
      601 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
      661 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
      721 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
      781 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      841 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaga
      901 gtcctgagcg gaaagaacca gctgtggaat gtgtgtcagt taggtgtnga aagtcccagc
      961 tccccagcag cagaagtatg caaagcatgc atctcaatta gtcagcaacc agtgtggaaa
     1021 gtccncaggc tccccagcag gcanangtat g
//
