LOCUS       sequence-248253-        6523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pmyo-3::GFP1-10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   He S, Cuentas-Condori A, Miller DM
  TITLE     NATF (Native And Tissue-Specific Fluorescence): A Strategy for 
            Bright, Tissue-Specific GFP Labeling of Native Proteins in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Apr 5. pii: genetics.119.302063. doi: 
            10.1534/genetics.119.302063.
  PUBMED    30952669
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Apr 5. pii: genetics.119.302063. doi: 
            10.1534/genetics.119.302063."
COMMENT     Sequence Label: pSH86
FEATURES             Location/Qualifiers
     source          1..6523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     71..93
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     90..106
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     90..106
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2788..3429
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     protein_bind    complement(3431..3455)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4225..4245)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4253..4270)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4253..4269)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4262..4284)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4597..4619
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4657..4675)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4743..4847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4848..4916,4917..5708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5066..5085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5879..6467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6368..6387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      121 gctatcaact ttccaagctt gcatgcgtga ttatagtctc tgttttcgtt aatttaattt
      181 tgcttgataa ggctgcaaca aagatcaggt tgacatatat tttcagtaat ttattttaac
      241 ctgtactcta tcactgccgg ctataataag ttcttgaata aaataatttt cccgacaaaa
      301 catgagtatt tctttcgaaa ataaaagtgc aggctaatta gagattattc tgtaattaac
      361 tgcataattt gtcacgtgcc atagttttac attccactac gtcatagttc ttaaaatact
      421 aatctcctga aaatagaagt aggtgaagaa agtttaatta tcagttctaa aatgacaatt
      481 gatctttgga atatgttctg aaactaccga tcattgaaca gatgctattt gaatgatata
      541 gaattgtata tttgcaattt ctgaaacgcg ttcttaaagg cacacagatt aattcaaaag
      601 ggtctggccg caaaaaggtt tatggtggcc gattttgagt tttgtgtgtg attgcttttt
      661 cacaatcagt gttttcagga ttatgtgatg aactagatct tcaagtttcg ttacatttca
      721 tatgttttcg gaactcacga agtacatatt gggtattgtg ctcaaaaaat tcagcaatca
      781 gcttcgctcc gctgacttta gaacccaaaa aaatagtatg gccaaactga ctgtgttacg
      841 atcatttcaa tttttcaata catatttaag atttctaaga gtaagaaggt caaaaactgt
      901 tctggaatac atatatattt ttcaggttac aattagtcaa aaagtgcact gaaatatacg
      961 ttttaatttc acgaataacc caattagttc aatgtatttt tggtcaacca acgttaaagt
     1021 ttggcttcca accaattatc atttctgatc aaccacaatg ttttttcttt atctgcaagt
     1081 taatttttta tttttatcca gatgtttggc atatttttca attcttcact agcgcccact
     1141 tcttgcactt ccggcgccct gaatctaatg catctgttgc aagaattgaa agaccaatca
     1201 acacattgtt ttcttcacga gatactgaag aaaatgaata aaaacagaga aaaagagcca
     1261 tgtgattagt gacaactgtt gctaacagat accatagctt ggacttggta cgtgatggca
     1321 acgtatgggt caacaaaaat gattgcagag ggggtgcaaa acagtcaagt cgagaaaata
     1381 tgaaaaacag aaaacaaaga acagaaaaat gggtttgaga gtcagtataa tttataaaag
     1441 aaaaattgta catagaaatt aaccattttt gtagaagaag ttatttttca agcatcgtta
     1501 aaaattattc aaagcacctt atttcatatt taattttaaa catggttaaa tgaacaacac
     1561 ggtgcgcaat caggaaaact tgaaatctga aactgttgtt gtgatcttct tcgcaactgt
     1621 tcagatagca ctagtgtaat gttaagagtg cgcgaatata atggaatata atggatcaca
     1681 cctcctgcca tcaggtaaac gtctctgtta tcacatattt ccaactatta aatttttacc
     1741 ttttacagtt ttacattttt ttgaaaaaag taactttttg tcttcaaaat ccctgacgaa
     1801 aatatcaaat attttaatcg agactgcaga ggaaccgatt gatgatttgg aaaatccagc
     1861 tttacctgtg taagaactga aaagtttcat aaccctaggg tattcccagt tacattcccc
     1921 actggctaac aatagcaccc agtttttcat cacctttctt caaatttctc ggcgatttgt
     1981 taaaaacaaa atttgtgtcc cttctctgat atctctatgt ctctaaacac aagttcatcg
     2041 gaaaacgaag gagggtaggt gttggttggg ctcccgaagt gaaaatagaa gagcaagaat
     2101 agaatattag agagagagtg cagagagggc gggatagctc ccgggattcc gttttcttct
     2161 tctttatctt caacgatgat gtgtgtgcgt gttgtataga ttctgttgct cccccacaac
     2221 tcgctccgaa ggctcaatac aattcaattg atattggagg agagcctacc ggagtgggag
     2281 gataagaaga aacataagaa gaagaagaag aagaagcatg cttctggttt ttgatgctat
     2341 gaaaacggca caaaaagatg attgaggtcc cttttcaata ccttctctca tctttcaaat
     2401 cccattgaaa cctaaaactt ctcaccacgc tttaccattg ttctccaaaa acttatagca
     2461 atgtctataa cttttttatc tctgaaaagc agtgttccat ttttcttttt cctattttat
     2521 ttcaattgtt tctcacattt cgtttggatt ctttgcttgt caaccagctt cttcttccac
     2581 ttttaccgtc taattttcag ggcagggagc catcaaaccc acgaccacta gatccatcta
     2641 gaaatgaggc gcgcctctag aggatccccg ggattggcca aaggacccaa aggtatgttt
     2701 cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag cgtcgacggt
     2761 accggtaggt acaaaaaagc aggcttaatg tccaaaggag aagaactgtt taccggtgtt
     2821 gtgccaattt tggttgaact cgatggtgat gtcaacggac ataagttctc agtgagaggc
     2881 gaaggagaag gtgacgccac cattggaaaa ttgactctta aattcatctg tactactggt
     2941 aaacttcctg taccatggcc gactctcgta acaacgctta cgtacggagt tcagtgcttt
     3001 tcgagatacc cagaccatat gaaaagacat gactttttta agtcggctat gcctgaaggt
     3061 tacgtgcaag aaagaacaat ttcgttcaaa gatgatggaa aatataaaac tagagcagtt
     3121 gttaaatttg aaggagatac tttggttaac cgcattgaac tgaaaggaac agattttaaa
     3181 gaagatggta atattcttgg acacaaactc gaatacaatt ttaatagtca taacgtatac
     3241 atcactgctg ataagcaaaa gaacggaatt aaagcgaatt tcacagtacg ccataatgta
     3301 gaagatggca gtgttcaact tgccgaccat taccaacaaa acacccctat tggagacggt
     3361 ccggtacttc ttcctgataa tcactacctc tcaacacaaa cagtcctgag caaagatcca
     3421 aatgaaaaat acccagcttt cttgtacaaa gtgggtgata tctgagctcc gcatcggccg
     3481 ctgtcatcag atcgccatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca
     3541 acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa
     3601 acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt
     3661 gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc
     3721 cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt
     3781 ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta
     3841 tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct
     3901 catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat
     3961 caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt
     4021 ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga
     4081 tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg
     4141 ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc
     4201 caatatacca aacataactg tttccaactt tattatacat agttgataat tcactggccg
     4261 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4321 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4381 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     4441 tgtgcggtat ttcacaccgc atatggtgca ctctcagtgc aatctgctct gatgccgcat
     4501 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4561 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4621 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4681 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4741 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4801 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4861 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4921 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4981 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5041 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5101 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5161 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5221 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5281 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5341 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5401 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5581 cagcactggg gtcagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5761 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5821 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6001 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6481 cggccttttt acggttcctg gtcttttgct ggccttttgc tca
//
