LOCUS       sequence-248257-        3937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part P- Promoter (non-constitutive); Pmer; metal sensitive; 
            controlled by merR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-Pmer_P
FEATURES             Location/Qualifiers
     source          1..3937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    170..190
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             218..238
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             258..965
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             258..965
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(282..310)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     905..925
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1005..1099
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1172..1263
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1264..1332,1333..2124)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2128..2167
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2314..2422
                     /note="incP origin of transfer"
     rep_origin      2458..3046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2947..2966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3200..3217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3232..3372
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3298..3317)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3458..3480
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3474..3665)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3845..3931)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagatcgc ttgactccgt acatgagtac ggaagtaagg
      121 ttacgctatc caatttcaat tcgaatactt gagacctttc atcgcgacct actagtagcg
      181 gccgctgcag ggagtaactt ctgactgagt tgcatacttg tttaacttta agaaggagat
      241 atacaatggt agcccgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      301 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      361 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      421 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
      481 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      541 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      601 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      661 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      721 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
      781 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
      841 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagatc
      901 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
      961 acaaaggttc gtaacttctg actgagttgc acgctctagt cttggactcc tgttgataga
     1021 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc
     1081 gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc
     1141 ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat
     1201 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1261 agtatgagca ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg
     1321 ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt
     1381 gcgcgcgtgg gctatattga actggatctg aacagcggca aaattctgga atcttttcgt
     1441 ccggaagaac gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg
     1501 agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat
     1561 ctggtggaat atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa
     1621 ctgtgcagcg cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc
     1681 attggcggtc cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt
     1741 ctggatcgtt gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc
     1801 atgccggcag caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg
     1861 gcaagccgcc agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg
     1921 cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc
     1981 agccgtggca ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt
     2041 tataccaccg gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc
     2101 gcgagcctga ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt
     2161 ttttggacga cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat
     2221 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt
     2281 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc
     2341 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa
     2401 tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa aagatcaaag gatcttcttg
     2461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2581 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     3241 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     3301 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     3361 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3421 ctcccggcat ccgcttacag acaagctgtg acagtctccg ggagctgcat gtgtcagagg
     3481 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     3541 tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     3601 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     3661 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     3721 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     3781 tgtgagggta aacaactggc ggtatggatg cggcgggggc gcgcccagct gtctagggcg
     3841 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3901 agtctttcga ctgagccttt cgttttattt gatgcct
//
