LOCUS       sequence-248259-        2662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part R- RBS; B0032m Ribosome Binding Site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-B0032_R
FEATURES             Location/Qualifiers
     source          1..2662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    132..152
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      186..280
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        353..444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(445..513,514..1305)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1309..1348
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1495..1603
                     /note="incP origin of transfer"
     rep_origin      1639..2227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2128..2147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2381..2398
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2515..2536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2570..2656)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc ttactagagt cacacaggaa agtactaaat gtgagacctt
      121 tcatcgcgac ctactagtag cggccgctgc agggagttgt cttcgaagac ttcgctctag
      181 tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag
      241 aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc ccaataatta
      301 cgatttaaat tagtagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta
      361 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      421 caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc gctgattccg
      481 ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt gaaagtgaaa
      541 gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct gaacagcggc
      601 aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac ctttaaagtg
      661 ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact gggccgtcgt
      721 attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa acatctgacc
      781 gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga taacaccgcg
      841 gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt tctgcataac
      901 atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga agcgattccg
      961 aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg taaactgctg
     1021 acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat ggaagcggat
     1081 aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat tgcggataaa
     1141 agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc ggatggtaaa
     1201 ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga tgaacgtaac
     1261 cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga tacaattaaa
     1321 ggctcctttt ggagcctttt tttttggacg acccttgtcc ttttccgctg cataaccctg
     1381 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     1441 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     1501 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     1561 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg gccccgtaga
     1621 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1681 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1741 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1801 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1861 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1921 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1981 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2041 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2101 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2161 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2221 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2281 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     2341 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     2401 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     2461 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     2521 gagttagctc actcattagg caggcgcgcc cagctgtcta gggcggcgga tttgtcctac
     2581 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     2641 cctttcgttt tatttgatgc ct
//
