LOCUS       sequence-248265-        8834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB
  TITLE     Dynamic analysis of cytosolic glucose and ATP levels in yeast using 
            optical sensors.
  JOURNAL   Biochem J. 2010 Dec 1. 432(2):399-406.
  PUBMED    20854260
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2010 Dec 1. 432(2):399-406."
COMMENT     Sequence Label: pDR-GW AT1.03
FEATURES             Location/Qualifiers
     source          1..8834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(152..154,155..157,158..670)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             1250..1984
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     protein_bind    complement(2022..2046)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2108..2127)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2109..2127)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2275..2462
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2330..2354)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2474..2491)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2474..2490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2483..2505)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2926..4272
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4383..5186)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5187..5402)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5427..5445)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5513..5617
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5618..5686,5687..6478)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5836..5855)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6649..7237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7138..7157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7391..7408
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7525..7546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7561..7566,7567..7584,7585..7591)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7599..7615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7604..7626
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7623..7639
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7623..7639
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7669..8124)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7806..7827)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8018..8037
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taattggaaa ataaccattt tttctctctt ttatacacac attcaaaaga aagaaaaaaa
       61 atatacccca gcctcgacta gtgaattcgc ggccatcaca agtttgtaca aaaaagcagg
      121 cttcgtttaa acgggccctc tagactcgag tatggtgagc aagggcgagg agctgttcac
      181 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca ggttcagcgt
      241 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      301 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
      361 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      421 cgaaggctac gtccaggagc gtaccatctt cttcaaggac gacggcaact acaagacccg
      481 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      541 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa
      601 cgtctatatc accgccgaca agcagaagaa cggcatcaag gcccacttca agatccgcca
      661 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      721 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
      781 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccatcga
      841 tatgaaaact gtgaaagtga atataacaac ccctgatggg ccagtctacg acgctgatat
      901 cgagatggtg tccgtgcggg ccgagagtgg tgatctcggc atcctccccg gtcacattcc
      961 cacaaaggcc ccactgaaga tcggagctgt gcggctgaag aaggacggcc aaaccgagat
     1021 ggtcgcagtc tcaggcggca ctgttgaagt gcggcctgac cacgttacca ttaatgctca
     1081 agccgctgaa acagccgaag gaatcgacaa agagagagca gaagccgcaa gacagagggc
     1141 ccaggagcgg ctgaactctc aatccgatga caccgatatt cgccgggccg agctggcact
     1201 gcagagggcc ctgaacagac tggacgtggc tgggaaggca aacgaattca tggacggcgg
     1261 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1321 gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca acgagaagcg
     1381 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1441 gctgtacaag ggtggcagcg gtggcatggt gagcaagggc gaggagctgt tcaccggggt
     1501 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1561 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
     1621 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt
     1681 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1741 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1801 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1861 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1921 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     1981 cgagtaagct taagtttaaa ccgctgatca gcctcgactg cacccagctt tcttgtacaa
     2041 agtggtgtag ttaattcatg atggccgctg caggtcgacc tcgagggggg gcccggtacc
     2101 caattcgccc tatagtgagt cgtattacgc gcggatccag ctttggactt cttcgccaga
     2161 ggtttggtca agtctccaat caaggttgtc ggcttgtcta ccttgccaga aatttacgaa
     2221 aagatggaaa agggtcaaat cgttggtaga tacgttgttg acacttctaa ataagcgaat
     2281 ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa
     2341 ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg
     2401 taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc
     2461 ggcatgccaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     2521 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     2581 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     2641 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataatcgga tcgtacttgt
     2701 tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac agatagtata
     2761 tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt atgtatttcg
     2821 gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc atccccggtt
     2881 cattttctgc gtttccatct tgcacttcaa tagcatatct ttgttaacga agcatctgtg
     2941 cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     3001 agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc
     3061 tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     3121 atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa
     3181 gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca
     3241 aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac
     3301 tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt
     3361 ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg
     3421 cattttttca agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac
     3481 tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt
     3541 ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt
     3601 cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga
     3661 taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg
     3721 ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg
     3781 tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt
     3841 tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata
     3901 ctttctagct agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag
     3961 cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat
     4021 atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc
     4081 ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg
     4141 tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg
     4201 ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt
     4261 tgatattgga tcgatccgat gataagctgt caaacatgag aattgggtaa taactgatat
     4321 aattaaattg aagctctaat ttgtgagttt agtatacatg catttactta taatacagtt
     4381 ttttagtttt gctggccgca tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt
     4441 tcaccctcta ccttagcatc ccttcccttt gcaaatagtc ctcttccaac aataataatg
     4501 tcagatcctg tagagaccac atcatccacg gttctatact gttgacccaa tgcgtctccc
     4561 ttgtcatcta aacccacacc gggtgtcata atcaaccaat cgtaaccttc atctcttcca
     4621 cccatgtctc tttgagcaat aaagccgata acaaaatctt tgtcgctctt cgcaatgtca
     4681 acagtaccct tagtatattc tccagtagat agggagccct tgcatgacaa ttctgctaac
     4741 atcaaaaggc ctctaggttc ctttgttact tcttctgccg cctgcttcaa accgctaaca
     4801 atacctgggc ccaccacacc gtgtgcattc gtaatgtctg cccattctgc tattctgtat
     4861 acacccgcag agtactgcaa tttgactgta ttaccaatgt cagcaaattt tctgtcttcg
     4921 aagagtaaaa aattgtactt ggcggataat gcctttagcg gcttaactgt gccctccatg
     4981 gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac aaattttggg acctaatgct
     5041 tcaactaact ccagtaattc cttggtggta cgaacatcca atgaagcaca caagtttgtt
     5101 tgcttttcgt gcatgatatt aaatagcttg gcagcaacag gactaggatg agtagcagca
     5161 cgttccttat atgtagcttt cgacatgatt tatcttcgtt tcctgcatgt ttttgttctg
     5221 tgcagttggg ttaagaatac tgggcaattt catgtttctt caacactaca tatgcgtata
     5281 tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa
     5341 tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg
     5401 aaaagctaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5461 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5521 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5581 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5641 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5701 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5761 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5821 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5881 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5941 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6001 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6061 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6121 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6181 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6241 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6301 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6361 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6421 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6481 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6541 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6601 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6661 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6721 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6781 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6841 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6901 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6961 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7021 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7081 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7141 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7201 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7261 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7321 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7381 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7441 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7501 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7561 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7621 cacaggaaac agctatgacc atgattacgc caagcttacc gcatcaggaa attgtaagcg
     7681 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     7741 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     7801 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     7861 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     7921 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     7981 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     8041 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     8101 ttaatgcgcc gctacagggc gcgtccattc gccaagcttc ctgaaacgga gaaacataaa
     8161 caggcattgc tgggatcacc catacatcac tctgttttgc ctgacctttt ccggtaattt
     8221 gaaaacaaac ccggtctcga agcggagatc cggcgataat taccgcagaa ataaacccat
     8281 acacgagacg tagaaccagc cgcacatggc cggagaaact cctgcgagaa tttcgtaaac
     8341 tcgcgcgcat tgcatctgta tttcctaatg cggcacttcc aggcctcgat cgagaccgtt
     8401 tatccattgc ttttttgttg tctttttccc tcgttcacag aaagtctgaa gaagctatag
     8461 tagaactatg agcttttttt gtttctgttt tccttttttt tttttttacc tctgtggaaa
     8521 ttgttactct cacactcttt agttcgtttg tttgttttgt ttattccaat tatgaccggt
     8581 gacgaaacgt ggtcgatggt gggtaccgct tatgctcccc tccattagtt tcgattatat
     8641 aaaaaggcca aatattgtat tattttcaaa tgtcctatca ttatcgtcta acatctaatt
     8701 tctcttaaat tttttctctt tctttcctat aacaccaata gtgaaaatct ttttttcttc
     8761 tatatctaca aaaacttttt ttttctatca acctcgttga taaatttttt ctttaacaat
     8821 cgttaataat taat
//
