LOCUS       sequence-248277-        2461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhanced RNAi vector for C. elegans. Contains two T7 polymerase 
            termination sequences adjacent to the T7 promoter sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T
  TITLE     Highly efficient RNAi and Cas9-based auto-cloning systems for C. 
            elegans research.
  JOURNAL   Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 
            10.1093/nar/gky516.
  PUBMED    29924347
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516."
COMMENT     Sequence Label: T444T
FEATURES             Location/Qualifiers
     source          1..2461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(651..698)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        699..717
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     775..791
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     775..791
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(861..877)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(862..878)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(903..921)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      922..969
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1142..1163
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1326..1430
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1431..1499,1500..2291)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1649..1668)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt caaaaaaccc
      661 ctcaagaccc gtttagaggc cccaaggggt tatgctagta atacgactca ctatagggag
      721 accggcagat ctgatatcat cgatgaattc gagctccacc gcggtggcgg ccgctctaga
      781 actagtggat ccaccggttc catggctagc cacgtgacgc gtggatcccc cgggctgcag
      841 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccaattc
      901 gccctatagt gagtcgtatt actagcataa ccccttgggg cctctaaacg ggtcttgagg
      961 ggttttttgg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     1021 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     1081 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     1141 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     1201 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     1261 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     1321 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1381 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1441 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1501 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1561 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1621 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1681 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1741 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1801 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1861 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1921 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1981 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2041 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2101 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2161 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2221 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2281 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2341 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2401 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2461 c
//
