LOCUS       sequence-248307-       15573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211, 
            alpha-L-Galp_697, alpha-L-Araf_1120 and beta-Xylp_1710 as inserts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15573)
  TITLE     Expression of arabinoxylan debranching enzymes in 
            Thermoanaerobacterium thermosaccharolyticum- Bacterial expression 
            plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLL1275
FEATURES             Location/Qualifiers
     source          1..15573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9134..9161
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(9179..9195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9179..9195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9192..9214)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9203..9219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9227..9233,9234..9251,9252..9257))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9272..9293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9487..10075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9567..9586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10246..11037,11038..11106))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10869..10888
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11107..11211)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11279..11297
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11335..11357)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11457..11476
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11670..11692
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11685..11701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             12531..12539
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(14248..15042)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 gagtcgtgac taagaacgtc aaagtaatta acaatacagc tatttttctc atgcttttac
       61 ccctttcata aaatttaatt ttatcgttat cataaaaaat tatagacgtt atattgcttg
      121 ccgggatata gtgctgggca ttcgttggtg caaaatgttc ggagtaaggt ggatattgat
      181 ttgcatgttg atctattgca ttgaaatgat tagttatccg taaatattaa ttaatcatat
      241 cataaattaa ttatatcata attgttttga cgaatgaagg tttttggata aattatcaag
      301 taaaggaacg ctaaaaattt tggcgtaaaa tatcaaaatg accacttgaa ttaatatggt
      361 aaagtagata taatattttg gtaaacatgc cttcagcaag gttagattag ctgtttccgt
      421 ataaattaac cgtatggtaa aacggcagtc agaaaaataa gtcataagat tccgttatga
      481 aaatatactt cggtagttaa taataagaga tatgaggtaa gagatacaag ataagagata
      541 taaggtacga atgtataaga tggtgctttt aggcacacta aataaaaaac aaataaacga
      601 aaattttaag gaggacgaaa gatggcaaag ataacgattc tggaagacaa gataattttt
      661 gaaagaagag atgagcttac ggtaatagaa gcttatggag caaattgtct gcgttaccgt
      721 tcaacccgca atattaagct gtctgatgag aactggaccc tgctgccgcc gactacaaaa
      781 gctgattgta aaacctttgg aaatgaaaga gaagcaacga tagtaaacgg cgatatttcc
      841 gcaactataa gggacggctt tggctggacc ggagccagta taagctactt tcgcaagggg
      901 aagcagattc ttcgatcccg tgatgagggt gatccggtta cccgctttac ccacctgggc
      961 ggtaacaatt atcaggtgaa ggcgatattt gacagtaatc cgggcgagca tatatatggc
     1021 ctgggacagg agcaggagga tcagtttgac cgcaagggct gcacctgcaa tatcatgcat
     1081 tacaatacca agtcgacttt gccggttatt tattcgtctc tgggatatgg ctttttgtgg
     1141 aacaatcctg cacccggccg ttgtgagact acctttaatc atacaatgtg ggttgcggat
     1201 agtgcatatc agattgatta tcttgtatat gtcggggata ctccggctga cataatgaaa
     1261 atatactgtg atctgacagg ctatgccccg aaattccctg actgggcggc cggcttctgg
     1321 cagtcaaagc tccgttatga aagccaggag gatttgctgg aggtggcaag ggaatacaag
     1381 aaaagaggaa ttcctctcgc agcgatagta atagattatt tccactggac ggagcagggg
     1441 gaatggaaat ttgatcccaa tttgtggcct gatcccaagg caatgtgtga cgagcttgag
     1501 tcaatgggag taaagcccgt tgtatccatc tggcctacca ttaatcccaa gagtgagaat
     1561 tatcatgcaa tggataatga gaatatgctg gtgaggaccg agaacgggca gtatggcata
     1621 ttcaactttt acggcttaca gacctttata gatcccacca atcctaaaac agggcccttt
     1681 gtatggagca aggtaaggga gaattattat aagtacggaa ttaagaactt ttggctggat
     1741 gaggctgagc cggaggtgca tcctcagcag tttggcaatc tgcgttttta tataggtaac
     1801 ggagcccaga cggcattatt gtatccatat tattatgcaa agatatttta tgatggctta
     1861 agaagtgagg gagaagagga tattataatc cttacccggg cagcctatcc cggcagccaa
     1921 agatttggtt ccctggtatg gaacggagac atcccctcca ccttcaaggc tcttcgccag
     1981 agtgtaacca gcggcctttc catgtcaatg tgcggaatcc catggtggaa cagcgacata
     2041 ggcggcttcc attccgcaga tatagaaagc gactatttca gggagcttat agtcagatgg
     2101 ttccagttcg gcctctttag tccggttatg cgccttcacg gggccagaaa ccgtacaaaa
     2161 aaccaggttg accgccatcc gggcgtgaag gagagaagcg gcggagacaa tgaaatctgg
     2221 agcttcggtg aaagaaatta tcctattctt aagaatatta tagagcttag ggagaggctg
     2281 aagccttata ttctcaaata tatggatatc gccagcaaga ccggctcacc gattatgagg
     2341 ccgatgttct atgattttta tgatgacgag atttgctacc gacttgatga tcagtacatg
     2401 ttcggagagg atatactctt tgctcccatt ctggagcagg gagtgacaca gcgcagtgtc
     2461 tacctgccgg agggtaaatg ggttaatgta aatgataaaa aaacctacga gggcaggcaa
     2521 atgataactg caagggcaga gctgcatgag tttattgcct tcgtaagaag cggcagcgag
     2581 gtgctggagg tatttagcta attctaaata ggaggtgtaa agtatgctgg aaatattaag
     2641 atcaaacatt aagagtaggg ggattatcat gaaatactat gtagatgcta atgctgcaaa
     2701 agacggcaat ggctcacttg agaagccatt taagcgaatt agcgatgcag cgaagctggc
     2761 gcttcccggc gatgaggtgt tggtggcgcc tggggtttac cgggaatatg tagatccact
     2821 taatgcaggc actgaggatg cccggatcag ttatatcagc actacagctc ttggtgcagt
     2881 gattaccggt gccgaacagg taaagagctg gacacattat aaggataatg tatgggtatg
     2941 caaaatctca aacagtattt tcggtgatta caatccatat acaaccctgg tatttggaga
     3001 ctggtatttt gcaacaccaa ataagcacac cggctgcgta ttcctgaatg ataaggctat
     3061 gtatgaagca gctagtttgg aggaatgcat caagggtaat atttatgagt acagctgggt
     3121 accggaagac tctatatata agtggtacac cgaacaggat acggaaaaag acgagacaat
     3181 catttatgcc aattttcagg gactggaccc caataaggaa aatgtagaaa taactgttcg
     3241 tcgcagatgc tttatgccat ccaaaacagg tgtcggttat attacggtca gaggctttaa
     3301 aatcgacaag gcagctacaa cctgggcacc gcctgcagcc tttcaggatg gtatgatcgg
     3361 tcctcactgg agcaaaggct ggattatcga ggattgcgag atctcaaaca gcaagtgcgc
     3421 aggcatttct ttgggtaaat atcttgacac ggacaatgag cattatttca catataagca
     3481 tgtaaaaagc gccacacaaa tggaacgtga tgctgtctgc cgcggccagt atcatggctg
     3541 gcttaaggaa aaaatcggcg gccatatagt tcgccgctgc aacatccacc attgtgagca
     3601 gggaggtatt atcggccgaa tgggcggtgt gttcagcatc atagaggaca accatatcca
     3661 ccatatcaat aatatgatgg agcttggcgg agcagaaatc tcaggaatta agctgcatgc
     3721 tgcaattgat gtgctcattc ggcgtaacca tatccaccat tgtacaatgg gaatatggtg
     3781 tgactgggaa gcacagggta cccgcattag ccagaacctg ctccatgata accagaggcc
     3841 tccgtttgcc aagaatctac cgggcagcat gatgtcccag gatctgtttg tggaagtcag
     3901 ccatggacct actcttatcg acaacaatat cctgctttcg gatgtcagcc tccgctttgc
     3961 aacacaaggt gtggctatgg ttcacaatct tatctgcggc tcctttacca gtgtcggtgg
     4021 tggaaccaca tggcgctata caccatacca tatcccccac cgtaccgagg tcatgggctt
     4081 tatgaccatt cttcatggtg acaatcgttt ctacaacaat atcttcatcc agaaatggcc
     4141 taatgaagaa tataccacct atgacgatca ggatgcgcag aatgccttta gggaaaacag
     4201 acaggttggc actcatgtat ttgatgaata tccaagctat gatgaatgga tttcccagtt
     4261 cgatttttca cattatccca atatgagagc cctggagcct gttcattttg gccatctgcc
     4321 aatctggagc gatggaaatg tgtatctgaa cggtgccaaa gcgtggaaaa aggaagcaaa
     4381 cagcctggta attgaaaaaa cggatcagga gcttaaggtg gagctggtgg aaaaggacgg
     4441 ccagtattat ctgaacacta atatttttga ttacatcaag gatttcaatg cccgtatgat
     4501 aaacacagaa atactcggca aagcctttga gccggagcag tactttgaaa atgcggacgg
     4561 tacgccaatt cgctttgact cagacttctt cggaaatcat cgtggggttc atgtattggc
     4621 aggcccgttt gcatctgcct ctgaaaatat aaagctataa gatagcgcat ggaggataaa
     4681 aaatgatgaa ggaagataga tatgtgctgt gctatacacg agagccacag gaggatatta
     4741 tttattccaa caagctggct tacagtatgc atctggcata tagcgaaaat ggagtaaaat
     4801 atgagccact taaccacaat tctggagttt tgtttgcaaa agccactcat aatccggatg
     4861 gtacgatgac agcaaaaagc ctgaagtgcc cgtttttgtt ttacatggag gacaatacct
     4921 ttggtgttgt tgcaataagg acggaggcta acggagatta tgatccggaa tgcaggggca
     4981 aggtattgtt ctttacctca aaggatttga ttcagtacac cgaggttggc ctgatagatc
     5041 ttaatacgaa tgcatatatc agggatgtta cctgtaaata tgacaaggtt aaggaaagat
     5101 acataatatg ctggcgtgac gattacggta agtggtatca gagtgaaata gatgatttat
     5161 tttctcttaa gtgccagtct gaacccgtca gcataggagc agttggcttt gccagggtta
     5221 atactgaaat agaaggaata gtaccccgca ataccataaa ggtatctgaa gaaatcggat
     5281 acagattaaa gagcaagctg acaacacccc gcatggtcaa aatcgagctg cctgaaacct
     5341 taagtgcttc ttcacctgaa gagctgagca aaatgagatt taaagccatt tacagtgacg
     5401 gtaccagctc gatcaagtct atagactgga atactgagag ggttgattgg agcaggcccg
     5461 gcacctatga tataaagggt gtaattcatc aggatcattt tgaattcccc attgccatca
     5521 accgtgctga tccctgcatc cgcaaatgga atggcaagta ttattttgtg gcaactaatg
     5581 acgccgacgg caatcacacc atatatatca gggaagctga tactattccg ggacttgcaa
     5641 ctgctgagga gcacttaatt cttgactcct ccacctatga aggtatcggt ggtctgttat
     5701 gggctccgga gcttcatgtt ataggagagg atttgtatat cttccatgca gcaacaccgg
     5761 gagaattctt ccatgaggaa tcccatgtaa tgcaactgaa gcgtggcggc aatcctcttt
     5821 gcaaggagga ttggtcggca ccgaagagag tagttaaaaa ggatggttct tacctgtgtg
     5881 aggccggcaa ggttatatca cttgatatga cctgctttga gtggaataag gaatactatg
     5941 ttgcctggtc acaaagacag ttcctgccag ttgatcaggg agcttggctc tatatagcca
     6001 aaattgatcc tcaggagcca tggagattaa tcaccgatcc tgttctgctc agcaagcctg
     6061 aatatggttg ggcaaataat catacctttg ttgatgaagg accttttgcc ctgataacag
     6121 ataaaaaaat ctttcttacc ttctccagcg ctgcggttga tgccacctat acagttggcc
     6181 tgctggtggc tgagccgggt tctgatttgc tggatcccgc aagctggaca aagctgaatt
     6241 atcctttact gaccagcaga agcgtacctg gtcaattcgg accgggacat aactcctatg
     6301 taattgatga tgatggcatt gtatggaatg cttaccatgc cagaccagga gttaacggtc
     6361 ccagaagctc aggaataaga cgcgttcact ttgacataga cggatacccg gtgcttgatc
     6421 ttactgagga aagagacgta agcgcccgtc tgaagaatgt atcctgcagg cttatagtaa
     6481 aatagaaatt aatgtaagga ggaaaagaat tggcaagaga taacacatat gaaagaataa
     6541 gttctcgaaa aatatcaggt gaaagggcta cagtaatgga aatagtaagg attaagagca
     6601 gatactcata tatggacata tcgaagcgca gagaaatgat ttccgataga agcgttattt
     6661 ccactgcgcc tgcaactgac agggctgact atttttatac gggaaacggg cactactggc
     6721 tggagagcgc cggaggagta gagaaggaca cgattattgt aaccgaggag catctttatg
     6781 agcctctttg gagcaaaacg cctaatccgc ccgatttgcg cggatgtatt gatgatatcc
     6841 gcaggtgcgt cctggaggga aatcccgaaa aggcagatga gtatattgac agggcacagc
     6901 gcatggccgg ttacgataag ctcatgaaca tggacagcaa aattgtctac cccatcggct
     6961 cacccagagt gcacaaggcc ttcagctttg atgtcacaag agaaggacta gatacggaca
     7021 gtgtatataa ttatataaga tggctcgata tggagaccgg gcttattacg aaccactttg
     7081 agatagcggg tgaaagctat gaaagcagga tagcagcatc gtttgccgac aatttctctg
     7141 cttttgaatt taccgcaccc agaggcaagc ttaatatctc tctcgaaata aaactgcccg
     7201 gcgggccgaa tatgttcggt gtcccgacca tggtcggttc gtctcacaaa tggactgtta
     7261 aggaaaacag gcttatcctc agctggcaat ataatcccga actgacaagc aaggggtatt
     7321 atgccattat cgacgcctca tgtgaggacg gagagctaag catcagcagt gagaaaatag
     7381 aaacgaagaa ttcgacccga ctgtatattg ccgttaaggt cattaagata gaggataatt
     7441 tcgactttga tggatataag gtcttcgagg ctgagtttga taagacagac aaaaggcttg
     7501 gcaggctttt acaggggaat gcggccatcc tcggggagaa gatgaaccgc tcatatatta
     7561 ctctgggcaa gaaggaagac tgcttacttt cgggcgagga gctccttgag aaatgtcaca
     7621 gtgagaagga gctttatcca atgatgatgg ataagctgta cgacatggga aggttctacc
     7681 agattgttga tacgggcgac attccgccta tgtggggaca gcacaacatc aataccaatc
     7741 tccaggtatg cgcaggcaac aatacaggtc tctttgacga aatggatgtt tacttcaggt
     7801 actatgaaag caagtttgat gacttcagaa ccaatgccag gcttctcttt aatgcgagag
     7861 ggcttctggc aagtgttcac tgtgacccgg attcgggcct gctctatcat ttttcaaaga
     7921 cttatccgca ttactgctgg acagggtgtc tgggctggat atataacgaa ttctggggat
     7981 actatcttgt tacaggagat aagaactttc tggagaatag gattatcccg gcacttaagg
     8041 aaattgcact gttctttgag gattacgcat gccttaagga tgagaaggga aagagcatat
     8101 ttgttccttc gttctcgcct gaggatccca ctcctaatcc tgattactca acagttacct
     8161 gcaaggacat tcatcccacc agaatcaact cggtgatgga tatcgcaata tgccgcgagg
     8221 tgctcacaaa cctcattgaa ggctgcaaaa tactcggcat agaggaagaa aatattccaa
     8281 agtggaaaag acagctggag gagcttcccg tataccttac cgatgaggaa ggcggcctta
     8341 aggagtgggc atgggagtcg atagaggaga actataatca ccgacatgtt tctcatcatt
     8401 acgatatatg gccaggaaga gccgtatctg tggataggga gcccgagctg gcacgcgcaa
     8461 taaagatttc taacagaaag cgcgcccagc aggacgattc cgcacacgga atcattcaca
     8521 gggccttctg tgcgttaaga ctgaaggata aggaagaatt tatccagaac atgtcgcagc
     8581 tgattaatca cggctttgta agaagaaacc ttgcaacggc acatttccct tacagaggtc
     8641 aattccccga tcttcagggc gcaatgcctg ctcttctgat tgagatgtgc gtttactcag
     8701 agcccggatt tgtggagttc ttgccggctc ttcccgatga tttcgcgcag ggcaggcttg
     8761 tcggggtgtg gctctatacc tttgcaaagt tggaaagcat ggagtggagc gaaaaggaaa
     8821 tcaaggtatc cataacgtcc aacagggatc agaccatcac gtttatgtta agaaacagag
     8881 cggcaaaatt ctttgtaaat ggaaaagaat attatccctg tgacggaaaa atcgactacc
     8941 actttagcga gggcgaaaaa gctgatatct atattgagct gtgactgggc ctttcgtttt
     9001 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     9061 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     9121 catcaaatta agcagaaggc catcctgacg gatggccttt tagcttggcg taatcatggt
     9181 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9241 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9301 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcagatc tgcggcgagc
     9361 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9421 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9481 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9541 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9601 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9661 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9721 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9781 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9841 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9901 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     9961 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10021 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10081 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10141 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10201 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10261 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10321 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10381 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10441 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10501 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10561 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10621 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10681 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10741 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10801 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10861 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10921 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10981 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    11041 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11101 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11161 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11221 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    11281 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    11341 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    11401 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    11461 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    11521 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    11581 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    11641 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    11701 tgaattgaca aagttttcta tttgtgttaa cattgtttat ataatagtga acagtgttaa
    11761 gattaaatgt gaggtgtttg tatggatatt aatgattata aagagaaggg actttattta
    11821 ttaagtagta tggatgattt tattaaaatt aatgatttgt ttatgggtaa agttgtttct
    11881 cctggctatg ttgcttcggt ttttggtgtt tccaggtcta ctgttacaca atggattcaa
    11941 agacgtaaaa ttagagcttt taagtataaa ggtaaggaag gtgactatat ggttatacct
    12001 attgctgata ttattgatta caaaagattg agtaataatg attttattta tgataagtta
    12061 gtgaggtgat ttattttatg tttgacgata gctatgttgt taatgagtgt tcgtctaatg
    12121 ttagtgaaaa tgatagagat ttttgtagtt tggttggtcg ttttatgatt attaatggta
    12181 tagataagtt ggttattaag attaatagaa aatttaatag gaaatcttta agtttagatt
    12241 ttagtgttga tttattccct tctatcaaag tttctgaatt agtttttttt gatgagttta
    12301 acaaaacgtg tggtttttat ttttctttta attcttttac aatttttaag gcttttagag
    12361 atgttcataa tcataataaa atatcatttt attttgcata atttcgggtc tgggccgcag
    12421 accaggccca gtgctaacaa tattaatttt taatgttagg aattgtttaa ttcttaattg
    12481 tgtttttaaa ggtagaataa ttacccattc gccctttagc caacaaaaat taaggaggta
    12541 taaacatgga taaaatggat ttgattcttc aagatgaaag actgggtgag atatttaaag
    12601 atatagattt aacagataat gaaaagagat atcttaaatg gttatggaaa tgggattatg
    12661 aaacacgtga tacttttgta tcaatttttt tgaagctaaa aaatggtgga aaatgatttt
    12721 tttcttatct tgatatatta gaaaaaagcg tactcacgaa gtaagaattt gtaaaaaaag
    12781 aaggggggat ttttttggat gagagtttgt acaagcagat tttaagtaat attattatta
    12841 ctcgtgatta ttgtaaaaat gttttagata atataaagtt caatgaaaaa ataattgatt
    12901 attatgttat gttacaaaat gatgttttta ttgattttac taataaaata aattcaataa
    12961 gggattgtaa taaatattgg tatttggatg tttataaaaa gcagaaaata aaggatttta
    13021 aaaagactaa tttgtgtaaa gataagttct gtaataattg taagaaagtt aaacaggctt
    13081 caagaatgca aaaatatatt cctgaattac agaaatacaa agatggctta tatcatttta
    13141 tatttactgt tgaaaatgtg ccaggtagtg aattaagaga tactattgat aggttgttta
    13201 agtcttttaa gtcatttaca aggtatttaa gtggtaatct taaaataaaa ggtgttaatt
    13261 ttgataaatg gggttataaa ggctgtgtaa ggtctttaga ggtaacttat agtatgattg
    13321 ataatcatat tatgtatcat ccacacttgc atgttgcgat gatattagat cctttttacg
    13381 atggttttaa tgttgaaagg atgcatataa ttaataagtt tagttatagc tatggtgttt
    13441 taaaaaggtt gtttactgat gatgaattat taattcaaaa aatttggtat ttattgttta
    13501 ataatattga ggttaacatg gccaatataa ataatttaga ggatggttat tcttgtttag
    13561 ttaataagtt tagtgattat gattatgcgg agctgtttaa gtatatttgt aaaaatactg
    13621 atgaacaagg tttacttatg acttatgata tttttaaaga tttatatttt gcattacata
    13681 atgttcatca gatacaaggc tatggttgtt tatataatat aagagatgat actcaattag
    13741 atttaaaggt tgatgacatt tataatgatt tgattgattt attacaagtt acagaaaatc
    13801 ctatacagtc tatggaaact gtacaggatt tattaaagga tactgaatat acaataataa
    13861 gccgtaagcg tatatttaag tatctaacac aattatatca taaggattga tatttatacc
    13921 gtctgtcgga ctcatgcgga gggggacttg agggggtctc ccctcgcatt gtacgacaga
    13981 cggtattatt attatacaaa ttttttttat gtaatttttt ttgtgtaatt tttttataca
    14041 aataatattt caattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg
    14101 cacgatacaa attcctcgta ggcgctcggg acccctatct agcgaacttt tagaaaagat
    14161 ataaaacatc agagtatgga cagttgcgga tgtacttcag aaaagattag atgtctaaaa
    14221 agctttttag acatctaaat ctaggtacta aaacaattca tccagtaaaa tataatattt
    14281 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
    14341 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    14401 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    14461 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    14521 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    14581 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    14641 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    14701 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    14761 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    14821 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    14881 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    14941 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    15001 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    15061 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    15121 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttga
    15181 aagttggcgt ataacatagt atcgacggag ccgattttga aaccacaatt atgatagaat
    15241 ttacaagcta taaggttatt gtcctgggtt tcaagcatta gtccatgcaa gtttttatgc
    15301 tttgcccatt ctatagatat attgataagc gcgctgccta tgccttgccc cctgaaatcc
    15361 ttacatacgg cgatatcttc tatataaaag atatattatc ttatcagtat tgtcaatata
    15421 ttcaaggcaa tctgcctcct catcctcttc atcctcttcg tcttggtagc tttttaaata
    15481 tggcgcttca tagagtaatt ctgtaaaggt ccaattctcg ttttcatacc tcggtataat
    15541 cttacctatc acctcaaatg gttcgctggg ttt
//
