LOCUS       sequence-248309-       15189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211, 
            alpha-L-Galp_697, alpha-L-Araf_996 and beta-Xylp_1710 as inserts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15189)
  TITLE     Expression of arabinoxylan debranching enzymes in 
            Thermoanaerobacterium thermosaccharolyticum- Bacterial expression 
            plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLL1276
FEATURES             Location/Qualifiers
     source          1..15189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      8750..8777
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(8795..8811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8795..8811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8808..8830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8819..8835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8843..8849,8850..8867,8868..8873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8888..8909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9103..9691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9183..9202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9862..10653,10654..10722))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10485..10504
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10723..10827)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10895..10913
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10951..10973)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11073..11092
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11286..11308
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11301..11317
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             12147..12155
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(13864..14658)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 gagtcgtgac taagaacgtc aaagtaatta acaatacagc tatttttctc atgcttttac
       61 ccctttcata aaatttaatt ttatcgttat cataaaaaat tatagacgtt atattgcttg
      121 ccgggatata gtgctgggca ttcgttggtg caaaatgttc ggagtaaggt ggatattgat
      181 ttgcatgttg atctattgca ttgaaatgat tagttatccg taaatattaa ttaatcatat
      241 cataaattaa ttatatcata attgttttga cgaatgaagg tttttggata aattatcaag
      301 taaaggaacg ctaaaaattt tggcgtaaaa tatcaaaatg accacttgaa ttaatatggt
      361 aaagtagata taatattttg gtaaacatgc cttcagcaag gttagattag ctgtttccgt
      421 ataaattaac cgtatggtaa aacggcagtc agaaaaataa gtcataagat tccgttatga
      481 aaatatactt cggtagttaa taataagaga tatgaggtaa gagatacaag ataagagata
      541 taaggtacga atgtataaga tggtgctttt aggcacacta aataaaaaac aaataaacga
      601 aaattttaag gaggacgaaa gatggcaaag ataacgattc tggaagacaa gataattttt
      661 gaaagaagag atgagcttac ggtaatagaa gcttatggag caaattgtct gcgttaccgt
      721 tcaacccgca atattaagct gtctgatgag aactggaccc tgctgccgcc gactacaaaa
      781 gctgattgta aaacctttgg aaatgaaaga gaagcaacga tagtaaacgg cgatatttcc
      841 gcaactataa gggacggctt tggctggacc ggagccagta taagctactt tcgcaagggg
      901 aagcagattc ttcgatcccg tgatgagggt gatccggtta cccgctttac ccacctgggc
      961 ggtaacaatt atcaggtgaa ggcgatattt gacagtaatc cgggcgagca tatatatggc
     1021 ctgggacagg agcaggagga tcagtttgac cgcaagggct gcacctgcaa tatcatgcat
     1081 tacaatacca agtcgacttt gccggttatt tattcgtctc tgggatatgg ctttttgtgg
     1141 aacaatcctg cacccggccg ttgtgagact acctttaatc atacaatgtg ggttgcggat
     1201 agtgcatatc agattgatta tcttgtatat gtcggggata ctccggctga cataatgaaa
     1261 atatactgtg atctgacagg ctatgccccg aaattccctg actgggcggc cggcttctgg
     1321 cagtcaaagc tccgttatga aagccaggag gatttgctgg aggtggcaag ggaatacaag
     1381 aaaagaggaa ttcctctcgc agcgatagta atagattatt tccactggac ggagcagggg
     1441 gaatggaaat ttgatcccaa tttgtggcct gatcccaagg caatgtgtga cgagcttgag
     1501 tcaatgggag taaagcccgt tgtatccatc tggcctacca ttaatcccaa gagtgagaat
     1561 tatcatgcaa tggataatga gaatatgctg gtgaggaccg agaacgggca gtatggcata
     1621 ttcaactttt acggcttaca gacctttata gatcccacca atcctaaaac agggcccttt
     1681 gtatggagca aggtaaggga gaattattat aagtacggaa ttaagaactt ttggctggat
     1741 gaggctgagc cggaggtgca tcctcagcag tttggcaatc tgcgttttta tataggtaac
     1801 ggagcccaga cggcattatt gtatccatat tattatgcaa agatatttta tgatggctta
     1861 agaagtgagg gagaagagga tattataatc cttacccggg cagcctatcc cggcagccaa
     1921 agatttggtt ccctggtatg gaacggagac atcccctcca ccttcaaggc tcttcgccag
     1981 agtgtaacca gcggcctttc catgtcaatg tgcggaatcc catggtggaa cagcgacata
     2041 ggcggcttcc attccgcaga tatagaaagc gactatttca gggagcttat agtcagatgg
     2101 ttccagttcg gcctctttag tccggttatg cgccttcacg gggccagaaa ccgtacaaaa
     2161 aaccaggttg accgccatcc gggcgtgaag gagagaagcg gcggagacaa tgaaatctgg
     2221 agcttcggtg aaagaaatta tcctattctt aagaatatta tagagcttag ggagaggctg
     2281 aagccttata ttctcaaata tatggatatc gccagcaaga ccggctcacc gattatgagg
     2341 ccgatgttct atgattttta tgatgacgag atttgctacc gacttgatga tcagtacatg
     2401 ttcggagagg atatactctt tgctcccatt ctggagcagg gagtgacaca gcgcagtgtc
     2461 tacctgccgg agggtaaatg ggttaatgta aatgataaaa aaacctacga gggcaggcaa
     2521 atgataactg caagggcaga gctgcatgag tttattgcct tcgtaagaag cggcagcgag
     2581 gtgctggagg tatttagcta attctaaata ggaggtgtaa agtatgctgg aaatattaag
     2641 atcaaacatt aagagtaggg ggattatcat gaaatactat gtagatgcta atgctgcaaa
     2701 agacggcaat ggctcacttg agaagccatt taagcgaatt agcgatgcag cgaagctggc
     2761 gcttcccggc gatgaggtgt tggtggcgcc tggggtttac cgggaatatg tagatccact
     2821 taatgcaggc actgaggatg cccggatcag ttatatcagc actacagctc ttggtgcagt
     2881 gattaccggt gccgaacagg taaagagctg gacacattat aaggataatg tatgggtatg
     2941 caaaatctca aacagtattt tcggtgatta caatccatat acaaccctgg tatttggaga
     3001 ctggtatttt gcaacaccaa ataagcacac cggctgcgta ttcctgaatg ataaggctat
     3061 gtatgaagca gctagtttgg aggaatgcat caagggtaat atttatgagt acagctgggt
     3121 accggaagac tctatatata agtggtacac cgaacaggat acggaaaaag acgagacaat
     3181 catttatgcc aattttcagg gactggaccc caataaggaa aatgtagaaa taactgttcg
     3241 tcgcagatgc tttatgccat ccaaaacagg tgtcggttat attacggtca gaggctttaa
     3301 aatcgacaag gcagctacaa cctgggcacc gcctgcagcc tttcaggatg gtatgatcgg
     3361 tcctcactgg agcaaaggct ggattatcga ggattgcgag atctcaaaca gcaagtgcgc
     3421 aggcatttct ttgggtaaat atcttgacac ggacaatgag cattatttca catataagca
     3481 tgtaaaaagc gccacacaaa tggaacgtga tgctgtctgc cgcggccagt atcatggctg
     3541 gcttaaggaa aaaatcggcg gccatatagt tcgccgctgc aacatccacc attgtgagca
     3601 gggaggtatt atcggccgaa tgggcggtgt gttcagcatc atagaggaca accatatcca
     3661 ccatatcaat aatatgatgg agcttggcgg agcagaaatc tcaggaatta agctgcatgc
     3721 tgcaattgat gtgctcattc ggcgtaacca tatccaccat tgtacaatgg gaatatggtg
     3781 tgactgggaa gcacagggta cccgcattag ccagaacctg ctccatgata accagaggcc
     3841 tccgtttgcc aagaatctac cgggcagcat gatgtcccag gatctgtttg tggaagtcag
     3901 ccatggacct actcttatcg acaacaatat cctgctttcg gatgtcagcc tccgctttgc
     3961 aacacaaggt gtggctatgg ttcacaatct tatctgcggc tcctttacca gtgtcggtgg
     4021 tggaaccaca tggcgctata caccatacca tatcccccac cgtaccgagg tcatgggctt
     4081 tatgaccatt cttcatggtg acaatcgttt ctacaacaat atcttcatcc agaaatggcc
     4141 taatgaagaa tataccacct atgacgatca ggatgcgcag aatgccttta gggaaaacag
     4201 acaggttggc actcatgtat ttgatgaata tccaagctat gatgaatgga tttcccagtt
     4261 cgatttttca cattatccca atatgagagc cctggagcct gttcattttg gccatctgcc
     4321 aatctggagc gatggaaatg tgtatctgaa cggtgccaaa gcgtggaaaa aggaagcaaa
     4381 cagcctggta attgaaaaaa cggatcagga gcttaaggtg gagctggtgg aaaaggacgg
     4441 ccagtattat ctgaacacta atatttttga ttacatcaag gatttcaatg cccgtatgat
     4501 aaacacagaa atactcggca aagcctttga gccggagcag tactttgaaa atgcggacgg
     4561 tacgccaatt cgctttgact cagacttctt cggaaatcat cgtggggttc atgtattggc
     4621 aggcccgttt gcatctgcct ctgaaaatat aaagctataa gatagcgcat ggaggataaa
     4681 aaatgaaaaa gcaaggcttt aacccttatc ttccttcctg ggaatatatc cctgatggag
     4741 aaccctatgt atttaatgac agagtctatg tatatggctc ccatgaccgt ttcaatggat
     4801 acgcattctg cctgaatgat tatgtttgct ggtctgctcc tgttgacaac ctggctgatt
     4861 ggcgctatga aggtgtcata ttaagagctg aggatgtaga gaacaatgat gaccgcgaca
     4921 gctgccttta tgctcctgat gttacggtag gtccggacgg aagatattac ttatattatg
     4981 ttgacagcag gcgttcaata gtatctgttg cagttagtga tactcctgca ggatattata
     5041 atttttatgg atatgttcat tatcccgacg gaacacgtct gggtgagcgg gaaggcgacg
     5101 agccacaatt tgatccggct gttctcacag agggtgacaa aacctatctc tatacaggct
     5161 tctgcgcaag aggagacaaa tccagacacg gtgccatggc aaccgtactt ggtccggata
     5221 tgctgactat tatagaagaa ccggtattta tcgtacccag ccagccatac agtgaaggca
     5281 caggctatga gggacatgaa ttctttgaag ctccttccat caggaagcgt ggagatacat
     5341 attactttat atactcctca attgtaatgc atgagctgtg ttatgccacc agcaagcatc
     5401 cgaccaagga tttcaaatac ggcggtgtta tcgttagcaa ctgcgatctc catatcgaca
     5461 gctacaagcc tgccaatatg atggcttact accctggcaa caatcacgga agcatagtac
     5521 agattaagga caaatggtat atcttctacc acagacatac taacggaacc cagtacagca
     5581 gacagggctg catggaggaa atcaaattcc tcgaggacgg ctctatacct caggttgaaa
     5641 taacctcctg cggctcaaac ggcggtcctc tggagggtaa aggcgaatat gccacttata
     5701 ttgcatgcaa tctcttcctt aaggaagatc ctaagcttcc cggcaacggc agatggccta
     5761 tgttctataa tccatatcct tatattaccc aggatggcaa ggatggcgac gaggagttgg
     5821 gttacattgc caacatgcaa tattccgcta cagcaggctt caagtatttt gactgcaagg
     5881 gaattaagaa gataaagatt aaggttcgcg gatattgccg cggtgacttt gaggtaagaa
     5941 ccaaatggga cggcccggta ctaggtacaa tcccggtagt attctccaac tgctggaagg
     6001 aatacagtgc cgatatagct attccggacg gcattaacgc cctctacttc acctatgcag
     6061 gaacaggaag cgccagcttt gcttccttta cactggaata aaaattaatg taaggaggaa
     6121 aagaattggc aagagataac acatatgaaa gaataagttc tcgaaaaata tcaggtgaaa
     6181 gggctacagt aatggaaata gtaaggatta agagcagata ctcatatatg gacatatcga
     6241 agcgcagaga aatgatttcc gatagaagcg ttatttccac tgcgcctgca actgacaggg
     6301 ctgactattt ttatacggga aacgggcact actggctgga gagcgccgga ggagtagaga
     6361 aggacacgat tattgtaacc gaggagcatc tttatgagcc tctttggagc aaaacgccta
     6421 atccgcccga tttgcgcgga tgtattgatg atatccgcag gtgcgtcctg gagggaaatc
     6481 ccgaaaaggc agatgagtat attgacaggg cacagcgcat ggccggttac gataagctca
     6541 tgaacatgga cagcaaaatt gtctacccca tcggctcacc cagagtgcac aaggccttca
     6601 gctttgatgt cacaagagaa ggactagata cggacagtgt atataattat ataagatggc
     6661 tcgatatgga gaccgggctt attacgaacc actttgagat agcgggtgaa agctatgaaa
     6721 gcaggatagc agcatcgttt gccgacaatt tctctgcttt tgaatttacc gcacccagag
     6781 gcaagcttaa tatctctctc gaaataaaac tgcccggcgg gccgaatatg ttcggtgtcc
     6841 cgaccatggt cggttcgtct cacaaatgga ctgttaagga aaacaggctt atcctcagct
     6901 ggcaatataa tcccgaactg acaagcaagg ggtattatgc cattatcgac gcctcatgtg
     6961 aggacggaga gctaagcatc agcagtgaga aaatagaaac gaagaattcg acccgactgt
     7021 atattgccgt taaggtcatt aagatagagg ataatttcga ctttgatgga tataaggtct
     7081 tcgaggctga gtttgataag acagacaaaa ggcttggcag gcttttacag gggaatgcgg
     7141 ccatcctcgg ggagaagatg aaccgctcat atattactct gggcaagaag gaagactgct
     7201 tactttcggg cgaggagctc cttgagaaat gtcacagtga gaaggagctt tatccaatga
     7261 tgatggataa gctgtacgac atgggaaggt tctaccagat tgttgatacg ggcgacattc
     7321 cgcctatgtg gggacagcac aacatcaata ccaatctcca ggtatgcgca ggcaacaata
     7381 caggtctctt tgacgaaatg gatgtttact tcaggtacta tgaaagcaag tttgatgact
     7441 tcagaaccaa tgccaggctt ctctttaatg cgagagggct tctggcaagt gttcactgtg
     7501 acccggattc gggcctgctc tatcattttt caaagactta tccgcattac tgctggacag
     7561 ggtgtctggg ctggatatat aacgaattct ggggatacta tcttgttaca ggagataaga
     7621 actttctgga gaataggatt atcccggcac ttaaggaaat tgcactgttc tttgaggatt
     7681 acgcatgcct taaggatgag aagggaaaga gcatatttgt tccttcgttc tcgcctgagg
     7741 atcccactcc taatcctgat tactcaacag ttacctgcaa ggacattcat cccaccagaa
     7801 tcaactcggt gatggatatc gcaatatgcc gcgaggtgct cacaaacctc attgaaggct
     7861 gcaaaatact cggcatagag gaagaaaata ttccaaagtg gaaaagacag ctggaggagc
     7921 ttcccgtata ccttaccgat gaggaaggcg gccttaagga gtgggcatgg gagtcgatag
     7981 aggagaacta taatcaccga catgtttctc atcattacga tatatggcca ggaagagccg
     8041 tatctgtgga tagggagccc gagctggcac gcgcaataaa gatttctaac agaaagcgcg
     8101 cccagcagga cgattccgca cacggaatca ttcacagggc cttctgtgcg ttaagactga
     8161 aggataagga agaatttatc cagaacatgt cgcagctgat taatcacggc tttgtaagaa
     8221 gaaaccttgc aacggcacat ttcccttaca gaggtcaatt ccccgatctt cagggcgcaa
     8281 tgcctgctct tctgattgag atgtgcgttt actcagagcc cggatttgtg gagttcttgc
     8341 cggctcttcc cgatgatttc gcgcagggca ggcttgtcgg ggtgtggctc tatacctttg
     8401 caaagttgga aagcatggag tggagcgaaa aggaaatcaa ggtatccata acgtccaaca
     8461 gggatcagac catcacgttt atgttaagaa acagagcggc aaaattcttt gtaaatggaa
     8521 aagaatatta tccctgtgac ggaaaaatcg actaccactt tagcgagggc gaaaaagctg
     8581 atatctatat tgagctgtga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     8641 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
     8701 gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc
     8761 ctgacggatg gccttttagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8821 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8881 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8941 cgggaaacct gtcgtgccag cagatctgcg gcgagcggta tcagctcact caaaggcggt
     9001 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9541 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9961 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10021 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10141 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10741 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10801 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10861 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10921 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    10981 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11041 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11101 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11161 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11221 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11281 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgacaaagt tttctatttg
    11341 tgttaacatt gtttatataa tagtgaacag tgttaagatt aaatgtgagg tgtttgtatg
    11401 gatattaatg attataaaga gaagggactt tatttattaa gtagtatgga tgattttatt
    11461 aaaattaatg atttgtttat gggtaaagtt gtttctcctg gctatgttgc ttcggttttt
    11521 ggtgtttcca ggtctactgt tacacaatgg attcaaagac gtaaaattag agcttttaag
    11581 tataaaggta aggaaggtga ctatatggtt atacctattg ctgatattat tgattacaaa
    11641 agattgagta ataatgattt tatttatgat aagttagtga ggtgatttat tttatgtttg
    11701 acgatagcta tgttgttaat gagtgttcgt ctaatgttag tgaaaatgat agagattttt
    11761 gtagtttggt tggtcgtttt atgattatta atggtataga taagttggtt attaagatta
    11821 atagaaaatt taataggaaa tctttaagtt tagattttag tgttgattta ttcccttcta
    11881 tcaaagtttc tgaattagtt ttttttgatg agtttaacaa aacgtgtggt ttttattttt
    11941 cttttaattc ttttacaatt tttaaggctt ttagagatgt tcataatcat aataaaatat
    12001 cattttattt tgcataattt cgggtctggg ccgcagacca ggcccagtgc taacaatatt
    12061 aatttttaat gttaggaatt gtttaattct taattgtgtt tttaaaggta gaataattac
    12121 ccattcgccc tttagccaac aaaaattaag gaggtataaa catggataaa atggatttga
    12181 ttcttcaaga tgaaagactg ggtgagatat ttaaagatat agatttaaca gataatgaaa
    12241 agagatatct taaatggtta tggaaatggg attatgaaac acgtgatact tttgtatcaa
    12301 tttttttgaa gctaaaaaat ggtggaaaat gatttttttc ttatcttgat atattagaaa
    12361 aaagcgtact cacgaagtaa gaatttgtaa aaaaagaagg ggggattttt ttggatgaga
    12421 gtttgtacaa gcagatttta agtaatatta ttattactcg tgattattgt aaaaatgttt
    12481 tagataatat aaagttcaat gaaaaaataa ttgattatta tgttatgtta caaaatgatg
    12541 tttttattga ttttactaat aaaataaatt caataaggga ttgtaataaa tattggtatt
    12601 tggatgttta taaaaagcag aaaataaagg attttaaaaa gactaatttg tgtaaagata
    12661 agttctgtaa taattgtaag aaagttaaac aggcttcaag aatgcaaaaa tatattcctg
    12721 aattacagaa atacaaagat ggcttatatc attttatatt tactgttgaa aatgtgccag
    12781 gtagtgaatt aagagatact attgataggt tgtttaagtc ttttaagtca tttacaaggt
    12841 atttaagtgg taatcttaaa ataaaaggtg ttaattttga taaatggggt tataaaggct
    12901 gtgtaaggtc tttagaggta acttatagta tgattgataa tcatattatg tatcatccac
    12961 acttgcatgt tgcgatgata ttagatcctt tttacgatgg ttttaatgtt gaaaggatgc
    13021 atataattaa taagtttagt tatagctatg gtgttttaaa aaggttgttt actgatgatg
    13081 aattattaat tcaaaaaatt tggtatttat tgtttaataa tattgaggtt aacatggcca
    13141 atataaataa tttagaggat ggttattctt gtttagttaa taagtttagt gattatgatt
    13201 atgcggagct gtttaagtat atttgtaaaa atactgatga acaaggttta cttatgactt
    13261 atgatatttt taaagattta tattttgcat tacataatgt tcatcagata caaggctatg
    13321 gttgtttata taatataaga gatgatactc aattagattt aaaggttgat gacatttata
    13381 atgatttgat tgatttatta caagttacag aaaatcctat acagtctatg gaaactgtac
    13441 aggatttatt aaaggatact gaatatacaa taataagccg taagcgtata tttaagtatc
    13501 taacacaatt atatcataag gattgatatt tataccgtct gtcggactca tgcggagggg
    13561 gacttgaggg ggtctcccct cgcattgtac gacagacggt attattatta tacaaatttt
    13621 ttttatgtaa ttttttttgt gtaatttttt tatacaaata atatttcaat tcgagctcgg
    13681 tacccgggga tcctctagag tcgacctgca ggcatgcacg atacaaattc ctcgtaggcg
    13741 ctcgggaccc ctatctagcg aacttttaga aaagatataa aacatcagag tatggacagt
    13801 tgcggatgta cttcagaaaa gattagatgt ctaaaaagct ttttagacat ctaaatctag
    13861 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
    13921 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
    13981 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
    14041 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
    14101 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
    14161 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
    14221 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
    14281 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
    14341 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
    14401 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
    14461 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
    14521 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
    14581 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
    14641 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
    14701 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
    14761 aaataccaga aaacagcttt ttcaaagttg ttttgaaagt tggcgtataa catagtatcg
    14821 acggagccga ttttgaaacc acaattatga tagaatttac aagctataag gttattgtcc
    14881 tgggtttcaa gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg
    14941 ataagcgcgc tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata
    15001 taaaagatat attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc
    15061 ctcttcatcc tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt
    15121 aaaggtccaa ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc
    15181 gctgggttt
//
