LOCUS       sequence-248360-        2660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part R- RBS; B0033m Ribosome Binding Site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-B0033_R
FEATURES             Location/Qualifiers
     source          1..2660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    130..150
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      184..278
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        351..442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(443..511,512..1303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1307..1346
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1493..1601
                     /note="incP origin of transfer"
     rep_origin      1637..2225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2126..2145
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2379..2396
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2513..2534
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2568..2654)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc ttactagagt cacacaggac tactaaatgt gagacctttc
      121 atcgcgacct actagtagcg gccgctgcag ggagttgtct tcgaagactt cgctctagtc
      181 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      241 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
      301 atttaaatta gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt
      361 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      421 ataatattga aaaaggaaga gtatgagcat tcagcatttt cgtgtggcgc tgattccgtt
      481 ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa accctggtga aagtgaaaga
      541 tgcggaagat caactgggtg cgcgcgtggg ctatattgaa ctggatctga acagcggcaa
      601 aattctggaa tcttttcgtc cggaagaacg ttttccgatg atgagcacct ttaaagtgct
      661 gctgtgcggt gcggttctga gccgtgtgga tgcgggccag gaacaactgg gccgtcgtat
      721 tcattatagc cagaacgatc tggtggaata tagcccggtg accgaaaaac atctgaccga
      781 tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc atgagcgata acaccgcggc
      841 gaacctgctg ctgacgacca ttggcggtcc gaaagaactg accgcgtttc tgcataacat
      901 gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa ctgaacgaag cgattccgaa
      961 cgatgaacgt gataccacca tgccggcagc aatggcgacc accctgcgta aactgctgac
     1021 gggtgagctg ctgaccctgg caagccgcca gcaactgatt gattggatgg aagcggataa
     1081 agtggcgggt ccgctgctgc gtagcgcgct gccggctggc tggtttattg cggataaaag
     1141 cggtgcgggc gaacgtggca gccgtggcat tattgcggcg ctgggcccgg atggtaaacc
     1201 gagccgtatt gtggtgattt ataccaccgg cagccaggcg acgatggatg aacgtaaccg
     1261 tcagattgcg gaaattggcg cgagcctgat taaacattgg taaaccgata caattaaagg
     1321 ctccttttgg agcctttttt tttggacgac ccttgtcctt ttccgctgca taaccctgct
     1381 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     1441 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     1501 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     1561 tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc cccgtagaaa
     1621 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1681 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1741 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1801 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1861 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1921 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1981 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2041 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2101 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2161 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2221 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2281 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2341 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2401 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2461 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2521 gttagctcac tcattaggca ggcgcgccca gctgtctagg gcggcggatt tgtcctactc
     2581 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
     2641 tttcgtttta tttgatgcct
//
