LOCUS       sequence-248361-        2661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part R- RBS; B0034m Ribosome Binding Site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-B0034_R
FEATURES             Location/Qualifiers
     source          1..2661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             90..101
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    131..151
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      185..279
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        352..443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(444..512,513..1304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1308..1347
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1494..1602
                     /note="incP origin of transfer"
     rep_origin      1638..2226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2127..2146
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2380..2397
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2514..2535
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2569..2655)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc ttactagaga aagaggagaa atactaaatg tgagaccttt
      121 catcgcgacc tactagtagc ggccgctgca gggagttgtc ttcgaagact tcgctctagt
      181 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
      241 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac
      301 gatttaaatt agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat
      361 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      421 aataatattg aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg ctgattccgt
      481 tttttgcggc gttttgcctg ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag
      541 atgcggaaga tcaactgggt gcgcgcgtgg gctatattga actggatctg aacagcggca
      601 aaattctgga atcttttcgt ccggaagaac gttttccgat gatgagcacc tttaaagtgc
      661 tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta
      721 ttcattatag ccagaacgat ctggtggaat atagcccggt gaccgaaaaa catctgaccg
      781 atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat aacaccgcgg
      841 cgaacctgct gctgacgacc attggcggtc cgaaagaact gaccgcgttt ctgcataaca
      901 tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa gcgattccga
      961 acgatgaacg tgataccacc atgccggcag caatggcgac caccctgcgt aaactgctga
     1021 cgggtgagct gctgaccctg gcaagccgcc agcaactgat tgattggatg gaagcggata
     1081 aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa
     1141 gcggtgcggg cgaacgtggc agccgtggca ttattgcggc gctgggcccg gatggtaaac
     1201 cgagccgtat tgtggtgatt tataccaccg gcagccaggc gacgatggat gaacgtaacc
     1261 gtcagattgc ggaaattggc gcgagcctga ttaaacattg gtaaaccgat acaattaaag
     1321 gctccttttg gagccttttt ttttggacga cccttgtcct tttccgctgc ataaccctgc
     1381 ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga
     1441 ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg
     1501 ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac
     1561 ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa
     1621 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1681 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1741 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1801 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1861 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1921 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1981 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2041 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2101 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2161 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2221 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2281 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2341 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2401 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2461 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     2521 agttagctca ctcattaggc aggcgcgccc agctgtctag ggcggcggat ttgtcctact
     2581 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
     2641 ctttcgtttt atttgatgcc t
//
