LOCUS       sequence-248362-        2666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part R- RBS; pET vector Ribosome Binding Site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-RBS-pET_R
FEATURES             Location/Qualifiers
     source          1..2666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             85..105
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    136..156
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      190..284
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        357..448
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(449..517,518..1309)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1313..1352
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1499..1607
                     /note="incP origin of transfer"
     rep_origin      1643..2231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2132..2151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2385..2402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2519..2540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2574..2660)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc ttacttgttt aactttaaga aggagatata caatgtgaga
      121 cctttcatcg cgacctacta gtagcggccg ctgcagggag ttgtcttcga agacttcgct
      181 ctagtcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
      241 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggg gtccccaata
      301 attacgattt aaattagtag cccgcctaat gagcgggctt ttttttaatt cccctatttg
      361 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      421 gcttcaataa tattgaaaaa ggaagagtat gagcattcag cattttcgtg tggcgctgat
      481 tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat ccggaaaccc tggtgaaagt
      541 gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat attgaactgg atctgaacag
      601 cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt ccgatgatga gcacctttaa
      661 agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg ggccaggaac aactgggccg
      721 tcgtattcat tatagccaga acgatctggt ggaatatagc ccggtgaccg aaaaacatct
      781 gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg attaccatga gcgataacac
      841 cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa gaactgaccg cgtttctgca
      901 taacatgggc gatcatgtga cccgtctgga tcgttgggaa ccggaactga acgaagcgat
      961 tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg gcgaccaccc tgcgtaaact
     1021 gctgacgggt gagctgctga ccctggcaag ccgccagcaa ctgattgatt ggatggaagc
     1081 ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg gctggctggt ttattgcgga
     1141 taaaagcggt gcgggcgaac gtggcagccg tggcattatt gcggcgctgg gcccggatgg
     1201 taaaccgagc cgtattgtgg tgatttatac caccggcagc caggcgacga tggatgaacg
     1261 taaccgtcag attgcggaaa ttggcgcgag cctgattaaa cattggtaaa ccgatacaat
     1321 taaaggctcc ttttggagcc tttttttttg gacgaccctt gtccttttcc gctgcataac
     1381 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata
     1441 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg
     1501 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt
     1561 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag gccggccccg
     1621 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1681 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1741 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1801 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1861 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1921 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1981 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2041 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2101 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2161 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2221 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2281 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2341 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2401 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2461 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2521 atgtgagtta gctcactcat taggcaggcg cgcccagctg tctagggcgg cggatttgtc
     2581 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     2641 tgagcctttc gttttatttg atgcct
//
