LOCUS       sequence-248366-        2668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part RN- RBS+N-terminus; B0033m Ribosome Binding Site + 
            Met-Val N-terminus start..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-B0032-MV_RN
FEATURES             Location/Qualifiers
     source          1..2668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    138..158
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      192..286
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        359..450
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(451..519,520..1311)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1315..1354
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1501..1609
                     /note="incP origin of transfer"
     rep_origin      1645..2233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2134..2153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2387..2404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2521..2542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2576..2662)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc ttactagagt cacacaggaa agtactaaat ggtagcctga
      121 gacctttcat cgcgacctac tagtagcggc cgctgcaggg agttgtcttc gaagacttcg
      181 ctctagtctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
      241 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccag gggtccccaa
      301 taattacgat ttaaattagt agcccgccta atgagcgggc ttttttttaa ttcccctatt
      361 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      421 atgcttcaat aatattgaaa aaggaagagt atgagcattc agcattttcg tgtggcgctg
      481 attccgtttt ttgcggcgtt ttgcctgccg gtgtttgcgc atccggaaac cctggtgaaa
      541 gtgaaagatg cggaagatca actgggtgcg cgcgtgggct atattgaact ggatctgaac
      601 agcggcaaaa ttctggaatc ttttcgtccg gaagaacgtt ttccgatgat gagcaccttt
      661 aaagtgctgc tgtgcggtgc ggttctgagc cgtgtggatg cgggccagga acaactgggc
      721 cgtcgtattc attatagcca gaacgatctg gtggaatata gcccggtgac cgaaaaacat
      781 ctgaccgatg gcatgaccgt gcgtgaactg tgcagcgcgg cgattaccat gagcgataac
      841 accgcggcga acctgctgct gacgaccatt ggcggtccga aagaactgac cgcgtttctg
      901 cataacatgg gcgatcatgt gacccgtctg gatcgttggg aaccggaact gaacgaagcg
      961 attccgaacg atgaacgtga taccaccatg ccggcagcaa tggcgaccac cctgcgtaaa
     1021 ctgctgacgg gtgagctgct gaccctggca agccgccagc aactgattga ttggatggaa
     1081 gcggataaag tggcgggtcc gctgctgcgt agcgcgctgc cggctggctg gtttattgcg
     1141 gataaaagcg gtgcgggcga acgtggcagc cgtggcatta ttgcggcgct gggcccggat
     1201 ggtaaaccga gccgtattgt ggtgatttat accaccggca gccaggcgac gatggatgaa
     1261 cgtaaccgtc agattgcgga aattggcgcg agcctgatta aacattggta aaccgataca
     1321 attaaaggct ccttttggag cctttttttt tggacgaccc ttgtcctttt ccgctgcata
     1381 accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata
     1441 tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc
     1501 gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta
     1561 ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgt aggccggccc
     1621 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1681 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1741 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1801 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1861 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1921 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1981 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2041 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2101 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2161 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2221 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2281 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2341 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2401 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     2461 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2521 taatgtgagt tagctcactc attaggcagg cgcgcccagc tgtctagggc ggcggatttg
     2581 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
     2641 actgagcctt tcgttttatt tgatgcct
//
