LOCUS       sequence-248368-        2667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + 
            Met-Val N-terminus start..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-B0034-MV_RN
FEATURES             Location/Qualifiers
     source          1..2667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             90..101
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    137..157
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      191..285
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        358..449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(450..518,519..1310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1314..1353
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1500..1608
                     /note="incP origin of transfer"
     rep_origin      1644..2232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2133..2152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2386..2403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2520..2541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2575..2661)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc ttactagaga aagaggagaa atactaaatg gtagcctgag
      121 acctttcatc gcgacctact agtagcggcc gctgcaggga gttgtcttcg aagacttcgc
      181 tctagtcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
      241 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccagg ggtccccaat
      301 aattacgatt taaattagta gcccgcctaa tgagcgggct tttttttaat tcccctattt
      361 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      421 tgcttcaata atattgaaaa aggaagagta tgagcattca gcattttcgt gtggcgctga
      481 ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca tccggaaacc ctggtgaaag
      541 tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta tattgaactg gatctgaaca
      601 gcggcaaaat tctggaatct tttcgtccgg aagaacgttt tccgatgatg agcaccttta
      661 aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc gggccaggaa caactgggcc
      721 gtcgtattca ttatagccag aacgatctgg tggaatatag cccggtgacc gaaaaacatc
      781 tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc gattaccatg agcgataaca
      841 ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa agaactgacc gcgtttctgc
      901 ataacatggg cgatcatgtg acccgtctgg atcgttggga accggaactg aacgaagcga
      961 ttccgaacga tgaacgtgat accaccatgc cggcagcaat ggcgaccacc ctgcgtaaac
     1021 tgctgacggg tgagctgctg accctggcaa gccgccagca actgattgat tggatggaag
     1081 cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc ggctggctgg tttattgcgg
     1141 ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat tgcggcgctg ggcccggatg
     1201 gtaaaccgag ccgtattgtg gtgatttata ccaccggcag ccaggcgacg atggatgaac
     1261 gtaaccgtca gattgcggaa attggcgcga gcctgattaa acattggtaa accgatacaa
     1321 ttaaaggctc cttttggagc cttttttttt ggacgaccct tgtccttttc cgctgcataa
     1381 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     1441 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
     1501 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
     1561 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgta ggccggcccc
     1621 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1681 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1741 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1801 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1861 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1921 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1981 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2041 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2101 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2161 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2221 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2281 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2341 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2401 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2461 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2521 aatgtgagtt agctcactca ttaggcaggc gcgcccagct gtctagggcg gcggatttgt
     2581 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
     2641 ctgagccttt cgttttattt gatgcct
//
