LOCUS       sequence-248372-        3299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part O- ORF; amilCP; blue chromoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-amilCP_O
FEATURES             Location/Qualifiers
     source          1..3299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    769..789
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      823..917
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        990..1081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1082..1150,1151..1942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1946..1985
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2132..2240
                     /note="incP origin of transfer"
     rep_origin      2276..2864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2765..2784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3018..3035
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3152..3173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3207..3293)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tagccagtgt gatcgctaaa caaatgacct acaaggttta
      121 tatgtcaggc acggtcaatg gacactactt tgaggtcgaa ggcgacggaa aaggtaagcc
      181 ctacgagggg gagcagacgg taaagctcac tgtcaccaag ggcggacctc tgccatttgc
      241 ttgggatatt ttatcaccac agtgtcagta cggaagcata ccattcacca agtaccctga
      301 agatatccct gactatgtaa agcagtcatt cccggagggc tatacatggg agaggatcat
      361 gaactttgaa gatggtgcag tgtgtactgt cagcaatgat tccagcatcc aaggcaactg
      421 tttcatctac catgtcaagt tctctggttt gaactttcct cccaatggac ctgtcatgca
      481 gaagaaaaca cagggctggg aacccaacac tgagcgtctg tttgcacgag atggaatgct
      541 gctaggaaac aactttatgg ctctgaagtt agaaggaggc ggtcactatt tgtgtgaatt
      601 taaaactact tacaaggcaa agaagcctgt gaagatgcca gggtatcact atgttgaccg
      661 caaactggat gtaaccaatc acaacaagga ttacacttcg gttgagcagt gtgaaatttc
      721 cattgcacgc aaacctgtgg tcgcttcgtg agacctttca tcgcgaccta ctagtagcgg
      781 ccgctgcagg gagttgtctt cgaagacttc gctctagtct tggactcctg ttgatagatc
      841 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
      901 tttttattgg tgagaatcca ggggtcccca ataattacga tttaaattag tagcccgcct
      961 aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac attcaaatat
     1021 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1081 tatgagcatt cagcattttc gtgtggcgct gattccgttt tttgcggcgt tttgcctgcc
     1141 ggtgtttgcg catccggaaa ccctggtgaa agtgaaagat gcggaagatc aactgggtgc
     1201 gcgcgtgggc tatattgaac tggatctgaa cagcggcaaa attctggaat cttttcgtcc
     1261 ggaagaacgt tttccgatga tgagcacctt taaagtgctg ctgtgcggtg cggttctgag
     1321 ccgtgtggat gcgggccagg aacaactggg ccgtcgtatt cattatagcc agaacgatct
     1381 ggtggaatat agcccggtga ccgaaaaaca tctgaccgat ggcatgaccg tgcgtgaact
     1441 gtgcagcgcg gcgattacca tgagcgataa caccgcggcg aacctgctgc tgacgaccat
     1501 tggcggtccg aaagaactga ccgcgtttct gcataacatg ggcgatcatg tgacccgtct
     1561 ggatcgttgg gaaccggaac tgaacgaagc gattccgaac gatgaacgtg ataccaccat
     1621 gccggcagca atggcgacca ccctgcgtaa actgctgacg ggtgagctgc tgaccctggc
     1681 aagccgccag caactgattg attggatgga agcggataaa gtggcgggtc cgctgctgcg
     1741 tagcgcgctg ccggctggct ggtttattgc ggataaaagc ggtgcgggcg aacgtggcag
     1801 ccgtggcatt attgcggcgc tgggcccgga tggtaaaccg agccgtattg tggtgattta
     1861 taccaccggc agccaggcga cgatggatga acgtaaccgt cagattgcgg aaattggcgc
     1921 gagcctgatt aaacattggt aaaccgatac aattaaaggc tccttttgga gccttttttt
     1981 ttggacgacc cttgtccttt tccgctgcat aaccctgctt cggggtcatt atagcgattt
     2041 tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag
     2101 actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg
     2161 cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc
     2221 ctgctctgcg aggctggccg taggccggcc ccgtagaaaa gatcaaagga tcttcttgag
     2281 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2341 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2401 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2461 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2521 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2581 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2641 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2701 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2761 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2821 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2881 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2941 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3001 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3061 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3121 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcag
     3181 gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa
     3241 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcct
//
